Abstract
The pathway from glucose 6-phosphate (G 6-P) to myoinositol 1-phosphate (Ins 1-P) and myo-inositol (Ins) is essential for the synthesis of various metabolites. In the halophyte Mesembryanthemum crystallinum (common ice plant), two enzymes, myo-inositol O-methyltransferase (IMT1) and ononitol epimerase (OEP1), extend this pathway and lead to the accumulation of methylated inositols, D-ononitol and D-pinitol, which serve as osmoprotectants. This paper describes transcripts for the enzyme, Inps1, encoding myo-inositol 1-phosphate synthase (INPS1), from the ice plant. Two Inps-like sequences are present in the genome. The deduced amino acid sequences of the cloned transcript are 49.5% and 87-90%, respectively, identical to those of yeast and other higher plant sequences. Inps1 RNA amounts are upregulated at least fivefold and amounts of free Ins accumulate approximately 10-fold during salinity stress. Inps1 induction is by transcription, similar to the induction of Imt1. In contrast, Arabidopsis thaliana does not show upregulation of Inps1 or increased amounts of Ins when salt-stressed. The lack of Inps1 induction in Arabidopsis exemplifies differences in glycophytic and halophytic regulation of gene expression at the point of entry into a pathway that leads to osmoprotection. The stress-induced coordinate upregulation of this pathway and its extension by novel enzymes in the ice plant also highlights biochemical differences.
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Claims 5
Claim · model-written summary
Two Inps-like sequences are present in the ice plant genome
- Taxon
- Mesembryanthemum crystallinum
Mesembryanthemum crystallinume0 - Outcome
- Inps-like sequencese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the halophyte Mesembryanthemum crystallinum (common ice plant)”
e1“Two Inps-like sequences are present in the genome”
Claim · model-written summary
Inps1 RNA amounts are upregulated at least fivefold in ice plant under salinity stress
- Taxon
- Mesembryanthemum crystallinumMesembryanthemum crystallinume0
- Treatment
- salinity stresse2
- Gene
- Inps1e2
- Outcome
- Inps1 RNA amountse2
- Direction
- upregulatede2
- Value
- at least fivefolde2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the halophyte Mesembryanthemum crystallinum (common ice plant)”
e2“Inps1 RNA amounts are upregulated at least fivefold and amounts of free Ins accumulate approximately 10-fold during salinity stress”
Claim · model-written summary
Free myo-inositol (Ins) accumulates about 10-fold in ice plant under salinity stress
- Taxon
- Mesembryanthemum crystallinumMesembryanthemum crystallinume0
- Treatment
- salinity stresse2
- Outcome
- amounts of free Inse2
- Direction
- accumulatee2
- Value
- approximately 10-folde2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the halophyte Mesembryanthemum crystallinum (common ice plant)”
e2“Inps1 RNA amounts are upregulated at least fivefold and amounts of free Ins accumulate approximately 10-fold during salinity stress”
Claim · model-written summary
Inps1 induction in ice plant occurs by transcription
- Taxon
- Mesembryanthemum crystallinumMesembryanthemum crystallinume0
- Gene
- Inps1e3
- Outcome
- Inps1 inductione3
- Qualifier
- by transcriptione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the halophyte Mesembryanthemum crystallinum (common ice plant)”
e3“Inps1 induction is by transcription”
Claim · model-written summary
Arabidopsis thaliana does not upregulate Inps1 or increase free Ins under salt stress
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae4 - Treatment
- salt-stressede4
- Outcome
- upregulation of Inps1 or increased amounts of Inse4
- Direction
- does not show upregulatione4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Arabidopsis thaliana does not show upregulation of Inps1 or increased amounts of Ins when salt-stressed”
The same questions in other papers
salinity · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
9 papers in the same direction as this paper · 10 in the opposite direction
- positive
- positive
- positive
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See all 20 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.