Paper · 1992 · American Journal of Botany

IN VITRO REJUVENATION OF BETULA (BETULACEAE): MORPHOLOGICAL EVALUATION

Mark H. Brand; R. Daniel Lineberger

6
claims
10
verbatim quotes
41
citations
3
research areas
—
Not open access

Abstract

The extent of rejuvenation that occurs during in vitro culture and propagation of Betula was determined. Comparisons were made between half‐sib seedlings and micropropagated, grafted, and cutting‐propagated mature‐phase birch plants grown under similar conditions. To measure relative levels of juvenility, three clones were examined at 1, 4, and 8 mo postpropagation, for differences in stem and leaf morphology and the presence or absence of reproductive structures. Numerous morphological indicators of phase change in Betula were identified. Micropropagated plants are not initially morphologically identical to conventionally propagated mature plants and display many features characteristic of seedlings. Micropropagated plants that exhibited juvenile morphology developed mature morphology more rapidly than did plants grown from seed. However, some mature‐phase morphological characteristics did not return to micropropagated plants during the time frame of the study. Rejuvenation of Betula appears to occur during in vitro micropropagation, but the level of juvenility that is regained may not be equivalent to that of a seedling. Not all morphological indicators of phase change are affected equally by in vitro rejuvenation.

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Claims 6

Observationobservedoriginal resultmeasured

Claim · model-written summary

Numerous morphological indicators of phase change in Betula were identified.

Taxon
BetulaBetulae0
Outcome
morphological indicators of phase changee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Betula
Betula · Taxon · picture from its Wikimedia Commons categoryShi Annan · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Numerous morphological indicators of phase change in Betula were identified.”

✓ located verbatim in the source · abstract, characters 469–545 · show in text ↑
Intervention effectobservedoriginal resultmixedmeasured

Claim · model-written summary

Micropropagated Betula plants initially differed morphologically from conventionally propagated mature plants and showed seedling features.

Taxon
BETULABetulat0
Treatment
Micropropagated plantse0
Outcome
many features characteristic of seedlingse0
Comparator
conventionally propagated mature plantse0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Betula
Betula · Taxon · picture from its Wikimedia Commons categoryShi Annan · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Micropropagated plants are not initially morphologically identical to conventionally propagated mature plants and display many features characteristic of seedlings.”

✓ located verbatim in the source · abstract, characters 546–710 · show in text ↑
t0

“BETULA (BETULACEAE)”

✓ located verbatim in the source · title, characters 25–44 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Micropropagated Betula plants with juvenile morphology developed mature morphology faster than seed-grown plants.

Taxon
BETULABetulat0
Treatment
Micropropagated plantse0
Outcome
developed mature morphologye0
Direction
more rapidlye0
Comparator
plants grown from seede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Betula
Betula · Taxon · picture from its Wikimedia Commons categoryShi Annan · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Micropropagated plants that exhibited juvenile morphology developed mature morphology more rapidly than did plants grown from seed.”

✓ located verbatim in the source · abstract, characters 711–842 · show in text ↑
t0

“BETULA (BETULACEAE)”

✓ located verbatim in the source · title, characters 25–44 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Some mature-phase characteristics did not return in micropropagated Betula during the study.

Taxon
BETULABetulat0
Outcome
mature‐phase morphological characteristics did not returne0
Qualifier
during the time frame of the studye0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Betula
Betula · Taxon · picture from its Wikimedia Commons categoryShi Annan · CC0 · Wikimedia Commons ↗

Verbatim

e0

“some mature‐phase morphological characteristics did not return to micropropagated plants during the time frame of the study.”

✓ located verbatim in the source · abstract, characters 852–976 · show in text ↑
t0

“BETULA (BETULACEAE)”

✓ located verbatim in the source · title, characters 25–44 · show in text ↑
Intervention effectauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

In vitro micropropagation appears to rejuvenate Betula, although regained juvenility may be below seedling levels.

Taxon
BetulaBetulae0
Treatment
in vitro micropropagatione0
Outcome
Rejuvenatione0
Qualifier
may not be equivalent to that of a seedlinge0
Comparator
a seedlinge0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Betula
Betula · Taxon · picture from its Wikimedia Commons categoryShi Annan · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Rejuvenation of Betula appears to occur during in vitro micropropagation, but the level of juvenility that is regained may not be equivalent to that of a seedling.”

✓ located verbatim in the source · abstract, characters 977–1140 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

In vitro rejuvenation affected Betula phase-change indicators unequally.

Taxon
BETULABetulat0
Outcome
morphological indicators of phase changee0
Qualifier
in vitro rejuvenatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Betula
Betula · Taxon · picture from its Wikimedia Commons categoryShi Annan · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Not all morphological indicators of phase change are affected equally by in vitro rejuvenation.”

✓ located verbatim in the source · abstract, characters 1141–1236 · show in text ↑
t0

“BETULA (BETULACEAE)”

✓ located verbatim in the source · title, characters 25–44 · show in text ↑