Abstract
Wheat seedlings exposed to 100 μM HgCl2 for 3 days exhibited high-level mercury (Hg) accumulation, which led to inhibited growth, increased lipid peroxidation, and disrupted cellular ultrastructures. And root growth and ultrastructural changes of wheat seedlings were inhibited more severely than those of leaves. To identify the wheat protein response to Hg stress, the iTRAQ method was used to determine the proteome profiles of the roots and leaves of wheat seedlings exposed to high-Hg conditions. 249 proteins were identified with significantly altered abundance. 117 were found in roots and 132 in leaves. These proteins were classified into signal transduction, stress defense, carbohydrate metabolism, protein metabolism, energy production, and transport functional groups. The majority of proteins identified in Hg-stressed roots and leaves displayed differently altered abundance, revealing organ-specific differences in adaption to Hg stress. Pathway Studio software was used to identify the Hg-responsive protein interaction network that included 49 putative key proteins, and they were potentially regulated by abscisic acid (ABA). Exogenous ABA application conferred protection against Hg stress and increased activities of peroxidase enzyme, suggesting that it may be an important factor in the Hg signaling pathway. These findings can provide useful insights into the molecular mechanisms of Hg responses in higher plants.
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Claims 13
Claim · model-written summary
Hg-exposed wheat seedlings accumulated high levels of mercury.
- Taxon
- Wheat
Triticum aestivume0 - Treatment
- 100 μM HgCl2 for 3 dayse0
- Outcome
- mercury (Hg) accumulatione0
- Direction
- high-levele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wheat seedlings exposed to 100 μM HgCl2 for 3 days exhibited high-level mercury (Hg) accumulation”
Claim · model-written summary
Hg accumulation inhibited seedling growth.
- Taxon
- WheatTriticum aestivume0
- Treatment
- 100 μM HgCl2 for 3 dayse0
- Outcome
- growthe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wheat seedlings exposed to 100 μM HgCl2 for 3 days exhibited high-level mercury (Hg) accumulation, which led to inhibited growth”
Claim · model-written summary
Hg exposure increased lipid peroxidation.
- Taxon
- WheatTriticum aestivume0
- Treatment
- 100 μM HgCl2 for 3 dayse0
- Outcome
- lipid peroxidatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wheat seedlings exposed to 100 μM HgCl2 for 3 days exhibited high-level mercury (Hg) accumulation, which led to inhibited growth, increased lipid peroxidation”
Claim · model-written summary
Hg exposure disrupted cellular ultrastructures.
- Taxon
- WheatTriticum aestivume0
- Treatment
- 100 μM HgCl2 for 3 dayse0
- Outcome
- cellular ultrastructurese0
- Direction
- disruptede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wheat seedlings exposed to 100 μM HgCl2 for 3 days exhibited high-level mercury (Hg) accumulation, which led to inhibited growth, increased lipid peroxidation, and disrupted cellular ultrastructures”
Claim · model-written summary
Root growth was inhibited more severely than leaves.
- Taxon
- wheatTriticum aestivume0
- Organ / stage
- roote0
- Outcome
- root growthe0
- Direction
- inhibited more severelye0
- Comparator
- leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“root growth and ultrastructural changes of wheat seedlings were inhibited more severely than those of leaves”
Claim · model-written summary
Root ultrastructural changes were more severe than leaf changes.
- Taxon
- wheatTriticum aestivume0
- Organ / stage
- roote0
- Outcome
- ultrastructural changese0
- Direction
- inhibited more severelye0
- Comparator
- leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“root growth and ultrastructural changes of wheat seedlings were inhibited more severely than those of leaves”
Claim · model-written summary
Hg exposure significantly altered abundance of 249 proteins.
- Taxon
- wheatTriticum aestivume1
- Outcome
- proteins were identified with significantly altered abundancee0
- Value
- 249 proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“249 proteins were identified with significantly altered abundance”
e1“wheat seedlings exposed to high-Hg conditions”
Claim · model-written summary
Of altered proteins, 117 were in roots.
- Taxon
- wheatTriticum aestivume1
- Organ / stage
- rootse0
- Outcome
- found in rootse0
- Value
- 117e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“117 were found in roots”
e1“wheat seedlings exposed to high-Hg conditions”
Claim · model-written summary
Of altered proteins, 132 were in leaves.
- Taxon
- wheatTriticum aestivume1
- Organ / stage
- leavese0
- Outcome
- in leavese0
- Value
- 132e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“132 in leaves”
e1“wheat seedlings exposed to high-Hg conditions”
Claim · model-written summary
Most Hg-responsive proteins showed organ-specific changes in abundance.
- Taxon
- wheatTriticum aestivume1
- Organ / stage
- roots and leavese0
- Outcome
- differently altered abundancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The majority of proteins identified in Hg-stressed roots and leaves displayed differently altered abundance”
e1“wheat seedlings exposed to high-Hg conditions”
Claim · model-written summary
The Hg-responsive protein network included 49 putative key proteins potentially regulated by ABA.
- Taxon
- wheatTriticum aestivume1
- Compound
- abscisic acid (ABA)e0
- Outcome
- Hg-responsive protein interaction networke0
- Value
- 49 putative key proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the Hg-responsive protein interaction network that included 49 putative key proteins, and they were potentially regulated by abscisic acid (ABA)”
e1“wheat seedlings exposed to high-Hg conditions”
Claim · model-written summary
Exogenous ABA protected wheat against Hg stress.
- Taxon
- WheatTriticum aestivume1
- Treatment
- Exogenous ABA applicatione0
- Outcome
- protection against Hg stresse0
- Direction
- conferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous ABA application conferred protection against Hg stress”
e1“Wheat seedlings”
Claim · model-written summary
Exogenous ABA increased peroxidase activity.
- Taxon
- WheatTriticum aestivume1
- Treatment
- Exogenous ABA applicatione0
- Gene
- peroxidase enzymee0
- Outcome
- activities of peroxidase enzymee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous ABA application conferred protection against Hg stress and increased activities of peroxidase enzyme”
e1“Wheat seedlings”
The same questions in other papers
(+)-abscisic acid · physiology: antioxidant enzymes · Triticum aestivum (wheat)Positive and negative results
16 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
14 papers more in this group
See all 18 papers →mercury dichloride · growth · Triticum aestivum (wheat)Results agree
2 papers in the same direction as this paper
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.