Abstract
Microtubules (MT) outline and maintain the overall shape of cells and can reorganize cellular membranes to serve as sites of RNA virus replication. Here, we provide data on involvement of an MT-associated protein in infection of plants with a potyvirus, Potato virus A (PVA), representing the largest family of plant-infecting RNA viruses. Our results showed that helper-component proteinase (HCpro)-interacting protein 2 (HIP2) of potato (Solanum tuberosum) is an MT-associated protein similar to Arabidopsis SPR2. Virus-induced silencing of HIP2 in Nicotiana benthamiana resulted in a spiral-like growth phenotype, similar to the Arabidopsis spr2 mutant, and the spr2 phenotype in Arabidopsis was complemented with potato HIP2. HCpro of PVA interacted with HIP2 of potato and tobacco (Nicotiana tabacum). The interaction was detected by bimolecular fluorescence complementation in PVA-infected leaves on MT and MT intersections at the cell cortex. HIP2-HCpro interaction was determined by the C-proximal α-helix-rich domain of HIP2, whereas the N-proximal putative TOG domain and the central coiled-coil domain of HIP2 controlled HIP2 dimerization and binding to MT. Accumulation of PVA was significantly reduced in the HIP2-silenced leaves of N. benthamiana, which indicates that HIP2-HCpro interactions are important for virus infection.
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Claims 8
Claim · model-written summary
Potato HIP2 was identified as a microtubule-associated protein similar to Arabidopsis SPR2.
- Taxon
- potato
Solanum tuberosume0 - Gene
- HIP2e0
- Outcome
- MT-associated protein similar to Arabidopsis SPR2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“helper-component proteinase (HCpro)-interacting protein 2 (HIP2) of potato (Solanum tuberosum) is an MT-associated protein similar to Arabidopsis SPR2”
Claim · model-written summary
HIP2 silencing in Nicotiana benthamiana produced a spiral-like growth phenotype.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- Virus-induced silencing of HIP2e0
- Gene
- HIP2e0
- Outcome
- spiral-like growth phenotypee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Virus-induced silencing of HIP2 in Nicotiana benthamiana resulted in a spiral-like growth phenotype, similar to the Arabidopsis spr2 mutant”
Claim · model-written summary
Potato HIP2 complemented the Arabidopsis spr2 phenotype.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- potato HIP2e0
- Gene
- potato HIP2e0
- Outcome
- spr2 phenotypee0
- Direction
- complementede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“spr2 phenotype in Arabidopsis was complemented with potato HIP2”
Claim · model-written summary
PVA HCpro interacted with HIP2 from potato and tobacco.
- Taxon
- potatoSolanum tuberosume0
- Interactor
- PVAPotato virus Ae0pathogen
- Gene
- HIP2e0
- Outcome
- HCpro of PVA interacted with HIP2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HCpro of PVA interacted with HIP2 of potato and tobacco (Nicotiana tabacum)”
Claim · model-written summary
The HIP2–HCpro interaction was detected at cortical microtubules and intersections in PVA-infected leaves.
- Taxon
- potatoSolanum tuberosume1
- Organ / stage
- leavese0
- Interactor
- PVAPotato virus Ae0pathogen
- Gene
- HIP2e1
- Outcome
- interaction was detectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“interaction was detected by bimolecular fluorescence complementation in PVA-infected leaves on MT and MT intersections at the cell cortex”
e1“HCpro of PVA interacted with HIP2 of potato and tobacco (Nicotiana tabacum)”
Claim · model-written summary
The C-proximal α-helix-rich HIP2 domain determined interaction with HCpro.
- Taxon
- potatoSolanum tuberosume1
- Interactor
- PVAPotato virus Ae1pathogen
- Gene
- HIP2e0
- Outcome
- HIP2-HCpro interactione0
- Qualifier
- C-proximal α-helix-rich domain of HIP2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HIP2-HCpro interaction was determined by the C-proximal α-helix-rich domain of HIP2”
e1“HCpro of PVA interacted with HIP2 of potato and tobacco (Nicotiana tabacum)”
Claim · model-written summary
The HIP2 N-proximal putative TOG domain and central coiled-coil domain controlled HIP2 dimerization and microtubule binding.
- Taxon
- potatoSolanum tuberosume1
- Gene
- HIP2e0
- Outcome
- HIP2 dimerization and binding to MTe0
- Qualifier
- N-proximal putative TOG domain and the central coiled-coil domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“N-proximal putative TOG domain and the central coiled-coil domain of HIP2 controlled HIP2 dimerization and binding to MT”
e1“protein 2 (HIP2) of potato (Solanum tuberosum)”
Claim · model-written summary
HIP2 silencing significantly reduced PVA accumulation in N. benthamiana leaves.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Organ / stage
- leavese0
- Treatment
- HIP2-silencede0
- Interactor
- PVAPotato virus Ae0pathogen
- Gene
- HIP2e0
- Outcome
- Accumulation of PVAe0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Accumulation of PVA was significantly reduced in the HIP2-silenced leaves of N. benthamiana”
The same questions in other papers
loss of function · growth · Nicotiana benthamianaPositive and negative results
16 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
17 papers more in this group
See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.