Abstract
Actinorhizal nodules do not usually evolve H 2 due to the action of an uptake hydrogenase. We have found that nodules of several Frankia symbioses evolved large amounts of H 2 gas when returned to air following exposure to 10 kPa C 2 HT 2 during an acetylene reduction assay. Increased H 2 evolution in air persisted for several days when intact root systems of Alnus incana (L.) Moench (inoculated with Frankia UGL 011101) were treated with 10 kPa C.H 2 for 1 h. Full recovery of uptake hydrogenase activity required 4 to 8 days. Studies with crude homogenates of nodules of the same plants showed that hydrogenase (measured amperometrically with phenazine metho‐sulfate as electron acceptor) was directly affected, since activity in treated nodules was only 10% of that in untreated nodules. A survey of actinorhizal symbioses revealed variation in the effect of an acetylene reduction assay on hydrogen metabolism. Nodules of three species, including Alnus rubra Bong, inoculated with Frankia HFPArD. showed complete inactivation of hydrogenase. H 2 evolution in air was 25% of the C 2 H 2 reduction rate and H, evolution in Ar/O 2 was equal to the QH 2 reduction rate. Two symbioses, Ceanothus americanus L. (soil inoculant) and Batista glomerata Baill. (soil inoculant) showed no change following an acetylene reduction assay. A third group of symbioses showed an intermediate response.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
After acetylene assay exposure, Frankia symbiosis nodules evolved large amounts of hydrogen in air.
- Taxon
- actinorhizal plantst0
- Treatment
- exposure to 10 kPa C 2 HT 2e0
- Outcome
- H 2 gase0
- Direction
- large amountse0
FrankiaNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nodules of several Frankia symbioses evolved large amounts of H 2 gas when returned to air following exposure to 10 kPa C 2 HT 2 during an acetylene reduction assay.”
t0“actinorhizal plants”
Claim · model-written summary
Acetylene treatment increased Alnus incana root hydrogen evolution for several days.
- Taxon
- Alnus incana
Alnus incanae0 - Treatment
- 10 kPa C.H 2 for 1 he0
- Interactor
- Frankia UGL 011101e0symbiont
- Outcome
- H 2 evolution in aire0
- Direction
- Increasede0
- Qualifier
- several dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increased H 2 evolution in air persisted for several days when intact root systems of Alnus incana (L.) Moench (inoculated with Frankia UGL 011101) were treated with 10 kPa C.H 2 for 1 h.”
Claim · model-written summary
Alnus incana uptake hydrogenase recovered fully in 4 to 8 days.
- Taxon
- Alnus incanaAlnus incanae1
- Gene
- uptake hydrogenasee0
- Outcome
- uptake hydrogenase activitye0
- Direction
- Full recoverye0
- Value
- 4 to 8 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Full recovery of uptake hydrogenase activity required 4 to 8 days.”
e1“Alnus incana (L.) Moench”
Claim · model-written summary
Hydrogenase activity in treated Alnus incana nodules was 10% of untreated nodules.
- Taxon
- Alnus incanaAlnus incanae2
- Treatment
- treated nodulese0
- Gene
- hydrogenasee1
- Outcome
- activitye0
- Direction
- onlye0
- Value
- 10%e0
- Comparator
- untreated nodulese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“activity in treated nodules was only 10% of that in untreated nodules.”
e1“hydrogenase (measured amperometrically with phenazine metho‐sulfate as electron acceptor)”
e2“Alnus incana (L.) Moench”
Claim · model-written summary
Three actinorhizal species including Alnus rubra had complete hydrogenase inactivation after assay.
- Taxon
- Alnus rubra
Alnus rubrae0 - Treatment
- acetylene reduction assaye1
- Interactor
- Frankia HFPArDe0symbiont
- Gene
- hydrogenasee0
- Outcome
- inactivation of hydrogenasee0
- Direction
- complete inactivatione0
- Value
- three speciese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nodules of three species, including Alnus rubra Bong, inoculated with Frankia HFPArD. showed complete inactivation of hydrogenase.”
e1“effect of an acetylene reduction assay on hydrogen metabolism.”
Claim · model-written summary
Hydrogen evolution in air equaled 25% of the acetylene reduction rate in the complete inactivation group.
- Taxon
- Alnus rubraAlnus rubrae1
- Outcome
- H 2 evolution in aire0
- Value
- 25%e0
- Comparator
- C 2 H 2 reduction ratee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H 2 evolution in air was 25% of the C 2 H 2 reduction rate”
e1“Alnus rubra Bong”
Claim · model-written summary
Hydrogen evolution in Ar/O2 equaled the reduction rate in the complete inactivation group.
- Taxon
- Alnus rubraAlnus rubrae1
- Outcome
- H, evolution in Ar/O 2e0
- Direction
- equal toe0
- Comparator
- QH 2 reduction ratee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H, evolution in Ar/O 2 was equal to the QH 2 reduction rate.”
e1“Alnus rubra Bong”
Claim · model-written summary
Ceanothus americanus symbiosis showed no change after acetylene assay.
- Taxon
- Ceanothus americanus
Ceanothus americanuse0 - Treatment
- acetylene reduction assaye0
- Interactor
- soil inoculante0symbiont
- Outcome
- no changee0
- Direction
- no changee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ceanothus americanus L. (soil inoculant) and Batista glomerata Baill. (soil inoculant) showed no change following an acetylene reduction assay.”
Claim · model-written summary
Batista glomerata symbiosis showed no change after acetylene assay.
- Taxon
- Batista glomeratae0
- Treatment
- acetylene reduction assaye0
- Interactor
- soil inoculante0symbiont
- Outcome
- no changee0
- Direction
- no changee0
Not reportedComparator
Verbatim
e0“Ceanothus americanus L. (soil inoculant) and Batista glomerata Baill. (soil inoculant) showed no change following an acetylene reduction assay.”
Claim · model-written summary
A third group of actinorhizal symbioses had an intermediate assay response.
Verbatim
e0“A third group of symbioses showed an intermediate response.”
t0“actinorhizal plants”