Abstract
Effect of salicylic acid (SA) on the expression of the potato proteinase inhibitor (PI) II promoter was studied with transgenic tobacco plants (Nicotiana tabacum) carrying a gene fusion between the PI-II promoter and the chloramphenicol acetyltransferase (cat) reporter. As previously observed, the PI-II promoter was inducible by wounding and the promoter activity was further enhanced by sucrose. Addition of SA did not influence the wound induction of the PI-II promoter but significantly inhibited the sucrose response. The 5'-deletion mutant -573 was unable to respond to wounding but did respond to sucrose and SA. The 3'-deletion analysis indicated the presence of a sucrose-responsive element between -574 and -520. A study of the insertion mutants revealed the function of another sucrose-responsive element between -522 and -500. Enhancer effects of these sucrose-responsive elements were inhibited by SA. These studies suggest that SA inhibits PI-II promoter activity by decreasing the sucrose response. Analysis of SA-related chemicals revealed that only acetyl-SA showed a similar inhibitory effect, and other hydroxybenzoic acids had little or no effect on the sucrose enhancer activity. Therefore, it seems that the interaction between SA and the receptor molecule is specific.
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Claims 12
Claim · model-written summary
Wounding induced PI-II promoter activity.
- Taxon
- tobacco
Nicotiana tabacumt0 - Treatment
- woundinge0
- Outcome
- PI-II promotere0
- Direction
- induciblee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the PI-II promoter was inducible by wounding”
t0“transgenic tobacco plants (Nicotiana tabacum)”
Claim · model-written summary
Sucrose further enhanced PI-II promoter activity.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- sucrosee0
- Outcome
- promoter activitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the promoter activity was further enhanced by sucrose”
t0“transgenic tobacco plants (Nicotiana tabacum)”
Claim · model-written summary
SA did not influence wound induction.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- SAe0
- Outcome
- wound induction of the PI-II promotere0
- Direction
- did not influencee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of SA did not influence the wound induction of the PI-II promoter”
t0“transgenic tobacco plants (Nicotiana tabacum)”
Claim · model-written summary
SA significantly inhibited the sucrose response.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- SAt1
- Outcome
- sucrose responsee0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“significantly inhibited the sucrose response”
t0“transgenic tobacco plants (Nicotiana tabacum)”
t1“Addition of SA”
Claim · model-written summary
The -573 deletion mutant failed to respond to wounding.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- 5'-deletion mutant -573e0
- Outcome
- unable to respond to woundinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 5'-deletion mutant -573 was unable to respond to wounding”
t0“transgenic tobacco plants (Nicotiana tabacum)”
Claim · model-written summary
The -573 deletion mutant responded to sucrose and SA.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- 5'-deletion mutant -573t1
- Outcome
- respond to sucrose and SAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“did respond to sucrose and SA”
t0“transgenic tobacco plants (Nicotiana tabacum)”
t1“5'-deletion mutant -573”
Claim · model-written summary
A sucrose-responsive element lies between -574 and -520.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- sucrose-responsive element between -574 and -520e0
- Outcome
- sucrose-responsive element between -574 and -520e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a sucrose-responsive element between -574 and -520”
t0“transgenic tobacco plants (Nicotiana tabacum)”
Claim · model-written summary
Another functional sucrose-responsive element lies between -522 and -500.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- sucrose-responsive element between -522 and -500e0
- Outcome
- function of another sucrose-responsive elemente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the function of another sucrose-responsive element between -522 and -500”
t0“transgenic tobacco plants (Nicotiana tabacum)”
Claim · model-written summary
SA inhibited enhancer effects of the sucrose-responsive elements.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- SAe0
- Outcome
- Enhancer effects of these sucrose-responsive elementse0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Enhancer effects of these sucrose-responsive elements were inhibited by SA”
t0“transgenic tobacco plants (Nicotiana tabacum)”
Claim · model-written summary
Acetyl-SA had a similar inhibitory effect.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- acetyl-SAe0
- Outcome
- inhibitory effecte0
- Direction
- inhibitorye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only acetyl-SA showed a similar inhibitory effect”
t0“transgenic tobacco plants (Nicotiana tabacum)”
Claim · model-written summary
Other hydroxybenzoic acids had little or no effect on sucrose enhancer activity.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- other hydroxybenzoic acidse0
- Outcome
- sucrose enhancer activitye0
- Direction
- little or no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“other hydroxybenzoic acids had little or no effect on the sucrose enhancer activity”
t0“transgenic tobacco plants (Nicotiana tabacum)”
Claim · model-written summary
The authors infer a specific SA–receptor interaction.
- Taxon
- tobaccoNicotiana tabacumt0
- Compound
- SAe0
- Outcome
- interaction between SA and the receptor molecule is specifice0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the interaction between SA and the receptor molecule is specific”
t0“transgenic tobacco plants (Nicotiana tabacum)”
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See all 36 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.