Abstract
Phytoremediation offers owners and managers of metal-contaminated sites an innovative and cost-effective option to address recalcitrant environmental contamination. The use of plants to restore or stabilize contaminated sites, known as phytoremediation, takes advantage of the natural abilities of plants to take up, accumulate or store metals. This includes the use of plants that tolerate and accumulate metals at high levels for phytoextraction and the use of plants growing under conditions that are toxic to other plants, for preventing, for example, soil erosion (phytostabilisation). Rapeseed (Brassica napus L.) was shown to be able to accumulate substantial amounts of metals combined with high biomass. Brassica napus was therefore selected for heavy metal (HM) tolerance and accumulation through in vitro selection. A selective pressure applied during the neoformation process from transversal thin cell layers (tTCLs) allowed us to select tolerant cells and tissues. Toxic metals (such as Zn) were added to the culture media in order to select zinc-tolerant plants. Exerting a selective pressure during tTCLs regeneration aimed at selecting plants with exceptional zinc tolerance and/or accumulating capacity. The morphological and physicochemical characteristics of regenerated plants cultivated in greenhouse appeared to depend very significantly on the concentration of ZnSO(4) applied during the neoformation process. Plants regenerated in the presence of ZnSO(4) at 100 microM exhibited a greater size and a higher biomass together with flowering precocity. The contents of zinc, chlorophyll, and proline were modified in the regenerated plants. Pre-treatment with an excess of ZnSO(4) (>500 microM) was responsible for a percentage of tTCLs intolerance above 96%. With lower Zn concentrations (100-250 microM), the survival rates (33-15%) were higher.
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Claims 7
Claim · model-written summary
Rapeseed was reported to combine substantial metal accumulation with high biomass.
Not reportedDirection
Verbatim
e0“Rapeseed (Brassica napus L.) was shown to be able to accumulate substantial amounts of metals combined with high biomass”
Claim · model-written summary
Regenerated plant morphology and physicochemical traits depended significantly on zinc sulfate concentration during neoformation.
- Taxon
- Rapeseede1
- Treatment
- concentration of ZnSO(4)e0
- Outcome
- morphological and physicochemical characteristicse0
- Setting
- greenhousee0
Not reportedDirection
Verbatim
e0“The morphological and physicochemical characteristics of regenerated plants cultivated in greenhouse appeared to depend very significantly on the concentration of ZnSO(4) applied during the neoformation process”
e1“Rapeseed (Brassica napus L.)”
Claim · model-written summary
Plants regenerated with 100 microM zinc sulfate were larger and had higher biomass.
- Taxon
- Rapeseede1
- Treatment
- ZnSO(4) at 100 microMe0
- Outcome
- greater size and a higher biomasse0
- Direction
- greatere0
Not reportedComparator
Verbatim
e0“Plants regenerated in the presence of ZnSO(4) at 100 microM exhibited a greater size and a higher biomass together with flowering precocity”
e1“Rapeseed (Brassica napus L.)”
Claim · model-written summary
Plants regenerated with 100 microM zinc sulfate flowered earlier.
Not reportedComparatorDirection
Verbatim
e0“Plants regenerated in the presence of ZnSO(4) at 100 microM exhibited a greater size and a higher biomass together with flowering precocity”
e1“Rapeseed (Brassica napus L.)”
Claim · model-written summary
Zinc, chlorophyll, and proline contents were modified in regenerated plants.
Not reportedDirection
Verbatim
e0“The contents of zinc, chlorophyll, and proline were modified in the regenerated plants”
e1“Rapeseed (Brassica napus L.)”
Claim · model-written summary
Pretreatment with over 500 microM zinc sulfate produced tissue intolerance above 96%.
- Taxon
- Rapeseede1
- Treatment
- ZnSO(4) (>500 microM)e0
- Outcome
- percentage of tTCLs intolerancee0
- Direction
- abovee0
- Value
- 96%e0
Not reportedComparator
Verbatim
e0“Pre-treatment with an excess of ZnSO(4) (>500 microM) was responsible for a percentage of tTCLs intolerance above 96%”
e1“Rapeseed (Brassica napus L.)”
Claim · model-written summary
At lower zinc concentrations of 100–250 microM, tissue survival rates were 33–15%.
- Taxon
- Rapeseede1
- Treatment
- Zn concentrations (100-250 microM)e0
- Outcome
- survival ratese0
- Direction
- highere0
- Value
- 33-15%e0
Not reportedComparator
Verbatim
e0“With lower Zn concentrations (100-250 microM), the survival rates (33-15%) were higher”
e1“Rapeseed (Brassica napus L.)”
The same questions in other papers
zinc atom · survival · Brassica napus (oilseed rape)Results agree
1 paper in the same direction as this paper
- positive
zinc sulfate · growth · Brassica napus (oilseed rape)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.