Paper · 2025 · Microscopy Research and Technique

Optimization of Phytosynthesized Zn–Se Nanocomposites via Response Surface Methodology: Characterization and Germination Impact on Gamma‐Irradiated Brassica napus L.

Zohaib Younas; Laiba Fatima; Ilyas Ahmad; et al.

8
claims
20
verbatim quotes
1
citations
2
research areas
✓
Open access

Abstract

The sustainable synthesis and optimization of nanocomposites (NCs) for agricultural applications remains a critical challenge. This study addresses this issue by developing Zn-Se NCs using green synthesis methods with Allium sativa and Mentha arvensis plant extracts optimized via Response Surface Methodology (RSM) to achieve an average size of 34.9 nm. For the first time, the combined application of NCs and gamma irradiation was evaluated to enhance seed viability and early growth in Brassica napus L. in the present study. The results indicated that p-values of 0.0001 and 0.0032 for the linear and quadratic regression models, respectively, were significant. The UV-visible spectrum of Zn-Se NCs showed a sharp peak at 295 nm, while FTIR spectra identified functional groups acting as a reducing agent, detailing their compound class, peak shape, and intensity. Spherical Zn-Se NCs were synthesized with an average size of 34.9 nm. The effects of optimized Zn-Se NCs on germination parameters were assessed, revealing that the percentage germination was non-significant, with a p-value > 0.05. In contrast, the survival rate, seedling length, and seed vigor index (SVI) were significant, with p-values of 0.002, 0.007, and 0.015, respectively. The results demonstrated significant improvements in germination percentage and seedling vigor, highlighting the potential of NCs as a nanobiofertilizer. This study presents a novel green synthesis approach for Zn-Se NCs using Allium sativa and Mentha arvensis plant extracts, achieving precise size control (< 35 nm) through RSM optimization, enhancing bioavailability and eco-friendliness. This study recommends the use of plant-mediated Zn-Se NCs in combination with gamma irradiation as a sustainable and effective approach to improve crop productivity and promote eco-friendly agricultural practices.

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Claims 8

Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Optimized Zn-Se nanocomposites had a non-significant effect on the percentage germination of Brassica napus.

Taxon
Brassica napusBrassica napust0
Treatment
optimized Zn-Se NCse1
Outcome
percentage germinatione1

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Brassica napus L.”

✓ located verbatim in the source · title, characters 148–165 · show in text ↑
e1

“The effects of optimized Zn-Se NCs on germination parameters were assessed, revealing that the percentage germination was non-significant, with a p-value > 0.05”

✓ located verbatim in the source · abstract, characters 939–1099 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Optimized Zn-Se nanocomposites significantly affected the survival rate of Brassica napus.

Taxon
Brassica napusBrassica napust0
Treatment
optimized Zn-Se NCse1
Outcome
survival ratee2

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Brassica napus L.”

✓ located verbatim in the source · title, characters 148–165 · show in text ↑
e1

“The effects of optimized Zn-Se NCs on germination parameters were assessed, revealing that the percentage germination was non-significant, with a p-value > 0.05”

✓ located verbatim in the source · abstract, characters 939–1099 · show in text ↑
e2

“In contrast, the survival rate, seedling length, and seed vigor index (SVI) were significant, with p-values of 0.002, 0.007, and 0.015, respectively”

✓ located verbatim in the source · abstract, characters 1101–1249 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Optimized Zn-Se nanocomposites significantly affected the seedling length of Brassica napus.

Taxon
Brassica napusBrassica napust0
Treatment
optimized Zn-Se NCse1
Outcome
seedling lengthe2

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Brassica napus L.”

✓ located verbatim in the source · title, characters 148–165 · show in text ↑
e1

“The effects of optimized Zn-Se NCs on germination parameters were assessed, revealing that the percentage germination was non-significant, with a p-value > 0.05”

✓ located verbatim in the source · abstract, characters 939–1099 · show in text ↑
e2

“In contrast, the survival rate, seedling length, and seed vigor index (SVI) were significant, with p-values of 0.002, 0.007, and 0.015, respectively”

✓ located verbatim in the source · abstract, characters 1101–1249 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Optimized Zn-Se nanocomposites significantly affected the seed vigour index of Brassica napus.

Taxon
Brassica napusBrassica napust0
Treatment
optimized Zn-Se NCse1
Outcome
seed vigor index (SVI)e2

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Brassica napus L.”

✓ located verbatim in the source · title, characters 148–165 · show in text ↑
e1

“The effects of optimized Zn-Se NCs on germination parameters were assessed, revealing that the percentage germination was non-significant, with a p-value > 0.05”

✓ located verbatim in the source · abstract, characters 939–1099 · show in text ↑
e2

“In contrast, the survival rate, seedling length, and seed vigor index (SVI) were significant, with p-values of 0.002, 0.007, and 0.015, respectively”

✓ located verbatim in the source · abstract, characters 1101–1249 · show in text ↑
Intervention effectauthor interpretationoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

The authors report significant improvements in germination percentage of Brassica napus with optimized Zn-Se nanocomposites.

Taxon
Brassica napusBrassica napust0
Treatment
optimized Zn-Se NCse1
Outcome
germination percentagee3
Direction
improvementse3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Brassica napus L.”

✓ located verbatim in the source · title, characters 148–165 · show in text ↑
e1

“The effects of optimized Zn-Se NCs on germination parameters were assessed, revealing that the percentage germination was non-significant, with a p-value > 0.05”

✓ located verbatim in the source · abstract, characters 939–1099 · show in text ↑
e3

“The results demonstrated significant improvements in germination percentage and seedling vigor”

✓ located verbatim in the source · abstract, characters 1251–1345 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors recommend plant-mediated Zn-Se nanocomposites with gamma irradiation to improve crop productivity.

Taxon
Brassica napusBrassica napust0
Treatment
plant-mediated Zn-Se NCse4
Outcome
crop productivitye4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Brassica napus L.”

✓ located verbatim in the source · title, characters 148–165 · show in text ↑
e4

“This study recommends the use of plant-mediated Zn-Se NCs in combination with gamma irradiation as a sustainable and effective approach to improve crop productivity”

✓ located verbatim in the source · abstract, characters 1643–1807 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Spherical Zn-Se nanocomposites were synthesized with an average size of 34.9 nm.

Taxon
Brassica napusBrassica napust0
Material
Zn-Se NCse5
Outcome
average sizee5
Value
34.9 nme5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Brassica napus L.”

✓ located verbatim in the source · title, characters 148–165 · show in text ↑
e5

“Spherical Zn-Se NCs were synthesized with an average size of 34.9 nm”

✓ located verbatim in the source · abstract, characters 869–937 · show in text ↑

schema checkedfield values found in the cited quotesPermalinkReport an issue

Observationobservedoriginal resultmeasured

Claim · model-written summary

Zn-Se nanocomposites showed a sharp UV-visible peak at 295 nm.

Taxon
Brassica napusBrassica napust0
Material
Zn-Se NCse6
Outcome
sharp peake6
Value
295 nme6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Brassica napus L.”

✓ located verbatim in the source · title, characters 148–165 · show in text ↑
e6

“The UV-visible spectrum of Zn-Se NCs showed a sharp peak at 295 nm”

✓ located verbatim in the source · abstract, characters 666–732 · show in text ↑

schema checkedfield values found in the cited quotesPermalinkReport an issue

zinc atom · growth · Brassica napus (oilseed rape)Results differ

20 papers: 15 positive, 5 negative or no effect

14 papers in the same direction as this paper · 5 in the opposite direction

  1. negative
  2. negative
  3. negative

16 papers more in this group

See all 20 papers →

zinc atom · survival · Brassica napus (oilseed rape)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

nanoparticle · growth · Brassica napus (oilseed rape)Positive and negative results

28 papers: 26 positive, 2 negative or no effect

25 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. negative
  3. positive

24 papers more in this group

See all 28 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.