Paper · 2000 · Canadian Journal of Microbiology

Plant growth-promoting bacteria that decrease heavy metal toxicity in plants

Genrich I Burd; D George Dixon; Bernard R Glick

4
claims
8
verbatim quotes
880
citations
2
research areas
—
Not open access

Abstract

Kluyvera ascorbata SUD165 and a siderophore-overproducing mutant of this bacterium, K. ascorbata SUD165/26, were used to inoculate tomato, canola, and Indian mustard seeds which were then grown in soil for 25-42 days in the presence of either nickel, lead, or zinc. The parameters that were monitored included plant wet and dry weight, protein and chlorophyll content in the plant leaves, and concentration of heavy metal in the plant roots and shoots. As indicated by a decrease in the measured values of these parameters, in all instances, plant growth was inhibited by the presence of the added metal. Both bacterial strains were effective, although not always to a statistically significant extent, at relieving a portion of the growth inhibition caused by the metals. In most cases, the siderophore overproducing mutant K. ascorbata 165/26 exerted a more pronounced effect on plant growth than did the wild-type bacterium K. ascorbata SUD165. The data suggest that the ability of these bacteria to protect plants against the inhibitory effects of high concentrations of nickel, lead, and zinc is related to the bacteria providing the plants with sufficient iron.

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Claims 4

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Plant growth of tomato, canola and Indian mustard was inhibited by added nickel, lead or zinc.

Treatment
nickel, lead, or zince0
Outcome
plant growthe1
Direction
inhibitede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica juncea
Brassica juncea · TaxonDalgial · CC BY-SA 3.0 · Wikimedia Commons ↗
Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Brassica napus var. napus
Brassica napus var. napus · Taxoncontri from Yonezawa-Shi, Yamagata, Japan · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“to inoculate tomato, canola, and Indian mustard seeds which were then grown in soil for 25-42 days in the presence of either nickel, lead, or zinc”

✓ located verbatim in the source · abstract, characters 118–264 · show in text ↑
e1

“plant growth was inhibited by the presence of the added metal”

✓ located verbatim in the source · abstract, characters 542–603 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Both K. ascorbata strains relieved part of the metal-caused growth inhibition in the inoculated plants, though not always to a statistically significant extent.

Treatment
Kluyvera ascorbata SUD165 and a siderophore-overproducing mutant of this bacterium, K. ascorbata SUD165/26e0
Interactor
Kluyvera ascorbata SUD165Kluyvera ascorbatae0other
Outcome
growth inhibitione1
Direction
relievinge1
Qualifier
although not always to a statistically significant extente1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica juncea
Brassica juncea · TaxonDalgial · CC BY-SA 3.0 · Wikimedia Commons ↗
Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Brassica napus var. napus
Brassica napus var. napus · Taxoncontri from Yonezawa-Shi, Yamagata, Japan · CC BY-SA 2.0 · Wikimedia Commons ↗
Kluyvera ascorbata
Kluyvera ascorbata · InteractorCDC · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Kluyvera ascorbata SUD165 and a siderophore-overproducing mutant of this bacterium, K. ascorbata SUD165/26, were used to inoculate tomato, canola, and Indian mustard seeds”

✓ located verbatim in the source · abstract, characters 0–171 · show in text ↑
e1

“Both bacterial strains were effective, although not always to a statistically significant extent, at relieving a portion of the growth inhibition caused by the metals”

✓ located verbatim in the source · abstract, characters 605–771 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

In most cases the siderophore-overproducing mutant K. ascorbata 165/26 had a more pronounced effect on plant growth than the wild-type K. ascorbata SUD165.

Treatment
siderophore overproducing mutant K. ascorbata 165/26e1
Interactor
K. ascorbata 165/26Kluyvera ascorbatae1other
Second interactor
K. ascorbata SUD165Kluyvera ascorbatae1other
Outcome
plant growthe1
Direction
more pronouncede1
Qualifier
In most casese1
Comparator
wild-type bacterium K. ascorbata SUD165e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica juncea
Brassica juncea · TaxonDalgial · CC BY-SA 3.0 · Wikimedia Commons ↗
Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Brassica napus var. napus
Brassica napus var. napus · Taxoncontri from Yonezawa-Shi, Yamagata, Japan · CC BY-SA 2.0 · Wikimedia Commons ↗
Kluyvera ascorbata
Kluyvera ascorbata · InteractorCDC · Public domain · Wikimedia Commons ↗

Verbatim

e0

“tomato, canola, and Indian mustard seeds”

✓ located verbatim in the source · abstract, characters 131–171 · show in text ↑
e1

“In most cases, the siderophore overproducing mutant K. ascorbata 165/26 exerted a more pronounced effect on plant growth than did the wild-type bacterium K. ascorbata SUD165”

✓ located verbatim in the source · abstract, characters 773–946 · show in text ↑
Mechanismauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

The authors suggest the protection against nickel, lead and zinc inhibition is related to the bacteria providing the plants with sufficient iron.

Compound
irone1
Outcome
protect plants against the inhibitory effectse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica juncea
Brassica juncea · TaxonDalgial · CC BY-SA 3.0 · Wikimedia Commons ↗
Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Brassica napus var. napus
Brassica napus var. napus · Taxoncontri from Yonezawa-Shi, Yamagata, Japan · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“tomato, canola, and Indian mustard seeds”

✓ located verbatim in the source · abstract, characters 131–171 · show in text ↑
e1

“The data suggest that the ability of these bacteria to protect plants against the inhibitory effects of high concentrations of nickel, lead, and zinc is related to the bacteria providing the plants with sufficient iron”

✓ located verbatim in the source · abstract, characters 948–1166 · show in text ↑

lead atom · growth · Brassica juncea (Indian mustard)Results differ

13 papers: 2 positive, 11 negative or no effect

10 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. positive
  3. positive

9 papers more in this group

See all 13 papers →

zinc atom · growth · Brassica napus (oilseed rape)Results differ

20 papers: 15 positive, 5 negative or no effect

4 papers in the same direction as this paper · 15 in the opposite direction

  1. positive
  2. positive
  3. positive

16 papers more in this group

See all 20 papers →

zinc atom · growth · Solanum lycopersicum (tomato)Results differ

57 papers: 36 positive, 21 negative or no effect

20 papers in the same direction as this paper · 36 in the opposite direction

  1. negative
  2. positive
  3. positive

53 papers more in this group

See all 57 papers →

nickel atom · growth · Brassica napus (oilseed rape)Positive and negative results

5 papers: 1 positive, 4 negative or no effect

3 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. negative

1 paper more in this group

See all 5 papers →

lead atom · growth · Brassica napus (oilseed rape)Positive and negative results

9 papers: 1 positive, 8 negative or no effect

7 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. negative

5 papers more in this group

See all 9 papers →

nickel atom · growth · Solanum lycopersicum (tomato)Positive and negative results

13 papers: 5 positive, 8 negative or no effect

7 papers in the same direction as this paper · 5 in the opposite direction

  1. positive
  2. positive
  3. positive

9 papers more in this group

See all 13 papers →

lead atom · growth · Solanum lycopersicum (tomato)Positive and negative results

18 papers: 1 positive, 17 negative or no effect

16 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. negative

14 papers more in this group

See all 18 papers →

zinc atom · growth · Brassica juncea (Indian mustard)Positive and negative results

20 papers: 17 positive, 3 negative or no effect

2 papers in the same direction as this paper · 17 in the opposite direction

  1. positive
  2. positive
  3. positive

16 papers more in this group

See all 20 papers →

8 of 12 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.