Abstract
Plant respiratory burst oxidase homolog (rboh) genes appear to play crucial roles in plant development, defense reactions and hormone signaling. In this study, a total of seven rboh genes from grape were identified and characterized. Genomic structure and predicted protein sequence analysis indicated that the sequences of plant rboh genes are highly conserved. Synteny analysis demonstrated that several Vvrboh genes were found in corresponding syntenic blocks of Arabidopsis, suggesting that these genes arose before the divergence of the respective lineages. The expression pattern of Vvrboh genes in different tissues was assessed by qRT-PCR and two were constitutively expressed in all tissues tested. The expression profiles were similarly analyzed following exposure to various stresses and hormone treatments. It was shown that the expression levels of VvrbohA, VvrbohB and VvrbohC1 were significantly increased by salt and drought treatments. VvrbohB, VvrbohC2, and VvrbohD exhibited a dramatic up-regulation after powdery mildew (Uncinula necator (Schw.) Burr.) inoculation, while VvrbohH was down-regulated. Finally, salicylic acid treatment strongly stimulated the expression of VvrbohD and VvrbohH, while abscisic acid treatment induced the expression of VvrbohB and VvrbohH. These results demonstrate that the expression patterns of grape rboh genes exhibit diverse and complex stress-response expression signatures.
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Claims 9
Claim · model-written summary
Seven grape rboh genes were identified and characterized.
Verbatim
e0“a total of seven rboh genes from grape were identified and characterized”
Claim · model-written summary
Plant rboh sequences were highly conserved.
Verbatim
e0“sequences of plant rboh genes are highly conserved”
t0“Grape”
t1“Grape”
Claim · model-written summary
Several Vvrboh genes occupy corresponding Arabidopsis syntenic blocks.
Verbatim
e0“several Vvrboh genes were found in corresponding syntenic blocks of Arabidopsis”
t0“Grape”
t1“Grape”
Claim · model-written summary
Two Vvrboh genes were constitutively expressed in tested tissues.
Verbatim
e0“The expression pattern of Vvrboh genes in different tissues was assessed by qRT-PCR and two were constitutively expressed in all tissues tested”
t0“Grape”
t1“Grape”
Claim · model-written summary
Salt and drought significantly increased VvrbohA, VvrbohB and VvrbohC1 expression.
- Taxon
- grapet0
- Treatment
- salt and drought treatmentse0
- Gene
- VvrbohA, VvrbohB and VvrbohC1e0
- Outcome
- expression levelse0
- Direction
- increasede0
Not reportedComparator
Verbatim
e0“expression levels of VvrbohA, VvrbohB and VvrbohC1 were significantly increased by salt and drought treatments”
t0“Grape”
t1“Grape”
Claim · model-written summary
Powdery mildew inoculation upregulated VvrbohB, VvrbohC2 and VvrbohD.
- Taxon
- grapet0
- Treatment
- powdery mildew (Uncinula necator (Schw.) Burr.) inoculatione0
- Interactor
- Uncinula necator (Schw.) Burr.
Erysiphe necatore0pathogen - Gene
- VvrbohB, VvrbohC2, and VvrbohDe0
- Outcome
- up-regulatione0
- Direction
- up-regulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VvrbohB, VvrbohC2, and VvrbohD exhibited a dramatic up-regulation after powdery mildew (Uncinula necator (Schw.) Burr.) inoculation”
t0“Grape”
t1“Grape”
Claim · model-written summary
Powdery mildew inoculation downregulated VvrbohH.
- Taxon
- grapet0
- Treatment
- powdery mildewe0
- Interactor
- powdery mildewe0pathogen
- Gene
- VvrbohHe0
- Outcome
- VvrbohHe0
- Direction
- down-regulatede0
Not reportedComparator
Verbatim
e0“powdery mildew (Uncinula necator (Schw.) Burr.) inoculation, while VvrbohH was down-regulated”
t0“Grape”
t1“Grape”
Claim · model-written summary
Salicylic acid stimulated VvrbohD and VvrbohH expression.
- Taxon
- grapet0
- Treatment
- salicylic acid treatmente0
- Gene
- VvrbohD and VvrbohHe0
- Outcome
- expressione0
- Direction
- stimulatede0
Not reportedComparator
Verbatim
e0“salicylic acid treatment strongly stimulated the expression of VvrbohD and VvrbohH”
t0“Grape”
t1“Grape”
Claim · model-written summary
Abscisic acid induced VvrbohB and VvrbohH expression.
- Taxon
- grapet0
- Treatment
- abscisic acid treatmente0
- Gene
- VvrbohB and VvrbohHe0
- Outcome
- expressione0
- Direction
- inducede0
Not reportedComparator
Verbatim
e0“abscisic acid treatment induced the expression of VvrbohB and VvrbohH”
t0“Grape”
t1“Grape”
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