Abstract
Salinity is one of the several abiotic factors affecting grape productivity and quality. Transcription factors belonging to AP2/ERF family play an important role in abiotic stress response. Based on the in silico analysis of salt stress libraries of grape ESTs, a transcription factor VviERF073, having a single AP2/ERF conserved domain was identified. The sequence of VviERF073 was analyzed in 'Thompson Seedless'. VviERF073 belonged to the B2 subfamily of ERF transcription factors. Under salt stress, the relative expression of VviERF073 increased 2.7 fold in the leaves of grafted grapevine within six hours of salt stress. The expression increased to 8.7 fold at 15 days of salt stress, thus indicating its role in early as well as late response to salt stress. However, expression of VviERF073 did not change significantly in grapevines raised on own roots. The expression varied in the roots of 'Thompson Seedless' but not in the roots of '110R'. Under salt stress conditions, GAPDH and EF1α for grafted vines and Actin and Tubulin for own root vines, were identified as the stable reference genes for qPCR data normalization.
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Claims 11
Claim · model-written summary
VviERF073 with a single AP2/ERF domain was identified in grape EST libraries.
Verbatim
e0“salt stress libraries of grape ESTs, a transcription factor VviERF073, having a single AP2/ERF conserved domain was identified”
Claim · model-written summary
VviERF073 belonged to the B2 subfamily of ERF transcription factors.
Verbatim
e0“VviERF073 belonged to the B2 subfamily of ERF transcription factors”
t0“grape”
Claim · model-written summary
VviERF073 expression increased 2.7 fold in grafted grapevine leaves within six hours of salt stress.
- Organ / stage
- leavese0
- Treatment
- salt stresse0
- Outcome
- relative expression of VviERF073e0
- Direction
- increasede0
- Value
- 2.7 folde0
- Qualifier
- within six hours of salt stresse0
VitisNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under salt stress, the relative expression of VviERF073 increased 2.7 fold in the leaves of grafted grapevine within six hours of salt stress”
Claim · model-written summary
VviERF073 expression increased 8.7 fold at 15 days of salt stress.
- Taxon
- grapet0
- Treatment
- salt stresse0
- Gene
- VviERF073e1
- Outcome
- expressione0
- Direction
- increasede0
- Value
- 8.7 folde0
- Qualifier
- 15 days of salt stresse0
Not reportedComparator
Verbatim
e0“The expression increased to 8.7 fold at 15 days of salt stress”
e1“relative expression of VviERF073 increased”
t0“grape”
Claim · model-written summary
VviERF073 expression did not change significantly in grapevines raised on own roots.
- Treatment
- salt stresse1
- Outcome
- expression of VviERF073e0
- Direction
- did not changee0
- Qualifier
- raised on own rootse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of VviERF073 did not change significantly in grapevines raised on own roots”
e1“Under salt stress, the relative expression”
Claim · model-written summary
Root expression of VviERF073 varied in Thompson Seedless.
Verbatim
e0“The expression varied in the roots of 'Thompson Seedless' but not in the roots of '110R'”
e1“expression of VviERF073 did not change”
t0“grape”
Claim · model-written summary
Root expression of VviERF073 did not vary in 110R.
Verbatim
e0“The expression varied in the roots of 'Thompson Seedless' but not in the roots of '110R'”
e1“expression of VviERF073 did not change”
t0“grape”
Claim · model-written summary
GAPDH was a stable qPCR reference gene for grafted vines under salt stress.
Not reportedComparator
Verbatim
e0“Under salt stress conditions, GAPDH and EF1α for grafted vines and Actin and Tubulin for own root vines, were identified as the stable reference genes for qPCR data normalization”
t0“grape”
Claim · model-written summary
EF1α was a stable qPCR reference gene for grafted vines under salt stress.
Not reportedComparator
Verbatim
e0“Under salt stress conditions, GAPDH and EF1α for grafted vines and Actin and Tubulin for own root vines, were identified as the stable reference genes for qPCR data normalization”
t0“grape”
Claim · model-written summary
Actin was a stable qPCR reference gene for own root vines under salt stress.
Not reportedComparator
Verbatim
e0“Under salt stress conditions, GAPDH and EF1α for grafted vines and Actin and Tubulin for own root vines, were identified as the stable reference genes for qPCR data normalization”
t0“grape”
Claim · model-written summary
Tubulin was a stable qPCR reference gene for own root vines under salt stress.
Not reportedComparator
Verbatim
e0“Under salt stress conditions, GAPDH and EF1α for grafted vines and Actin and Tubulin for own root vines, were identified as the stable reference genes for qPCR data normalization”
t0“grape”
The same questions in other papers
salinity · molecular: expression · VitisPositive and negative results
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- positive
- positive
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3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.