Abstract
Cys2/His2-type zinc finger proteins, which contain the EAR transcriptional repressor domain, are thought to play a key role in regulating the defense response of plants to biotic and abiotic stress conditions. Although constitutive expression of several of these proteins was shown to enhance the tolerance of transgenic plants to abiotic stress, it is not clear whether the EAR-motif of these proteins is involved in this function. In addition, it is not clear whether suppression of plant growth, induced in transgenic plants by different Cys2/His2 EAR-containing proteins, is mediated by the EAR-domain. Here we report that transgenic Arabidopsis plants constitutively expressing the Cys2/His2 zinc finger protein Zat7 have suppressed growth and are more tolerant to salinity stress. A deletion or a mutation of the EAR-motif of Zat7 abolishes salinity tolerance without affecting growth suppression. These results demonstrate that the EAR-motif of Zat7 is directly involved in enhancing the tolerance of transgenic plants to salinity stress. In contrast, the EAR-motif appears not to be involved in suppressing the growth of transgenic plants. Further analysis of Zat7 using RNAi lines suggests that Zat7 functions in Arabidopsis to suppress a repressor of defense responses. A yeast two-hybrid analysis identified putative interactors of Zat7 and the EAR-domain, including WRKY70 and HASTY, a protein involved in miRNA transport. Our findings demonstrate that the EAR-domain of Cys2/His2-type zinc finger proteins plays a key role in the defense response of Arabidopsis to abiotic stresses.
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Claims 6
Claim · model-written summary
Transgenic Arabidopsis plants constitutively expressing Zat7 have suppressed growth.
- Taxon
- Arabidopsis
Arabidopsise0 - Material
- transgenic Arabidopsis plantse0
- Treatment
- constitutively expressing the Cys2/His2 zinc finger protein Zat7e0
- Gene
- Cys2/His2 zinc finger protein Zat7e0
- Outcome
- growthe0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic Arabidopsis plants constitutively expressing the Cys2/His2 zinc finger protein Zat7 have suppressed growth and are more tolerant to salinity stress”
Claim · model-written summary
Transgenic Arabidopsis plants constitutively expressing Zat7 are more tolerant to salinity stress.
- Taxon
- ArabidopsisArabidopsise0
- Material
- transgenic Arabidopsis plantse0
- Treatment
- constitutively expressing the Cys2/His2 zinc finger protein Zat7e0
- Gene
- Cys2/His2 zinc finger protein Zat7e0
- Outcome
- salinity stresse0
- Direction
- more tolerante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic Arabidopsis plants constitutively expressing the Cys2/His2 zinc finger protein Zat7 have suppressed growth and are more tolerant to salinity stress”
Claim · model-written summary
A deletion or mutation of the EAR-motif of Zat7 abolishes salinity tolerance.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- A deletion or a mutation of the EAR-motif of Zat7e1
- Gene
- Zat7e1
- Outcome
- salinity tolerancee1
- Direction
- abolishese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic Arabidopsis plants constitutively expressing the Cys2/His2 zinc finger protein Zat7”
e1“A deletion or a mutation of the EAR-motif of Zat7 abolishes salinity tolerance without affecting growth suppression”
Claim · model-written summary
A deletion or mutation of the EAR-motif of Zat7 does not change growth suppression.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- A deletion or a mutation of the EAR-motif of Zat7e1
- Outcome
- growth suppressione1
- Direction
- without affectinge1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic Arabidopsis plants constitutively expressing the Cys2/His2 zinc finger protein Zat7”
e1“A deletion or a mutation of the EAR-motif of Zat7 abolishes salinity tolerance without affecting growth suppression”
Claim · model-written summary
Zat7 appears to function in Arabidopsis by suppressing a repressor of defense responses.
- Taxon
- ArabidopsisArabidopsise2
- Gene
- Zat7e2
- Outcome
- suppress a repressor of defense responsese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Further analysis of Zat7 using RNAi lines suggests that Zat7 functions in Arabidopsis to suppress a repressor of defense responses”
Claim · model-written summary
A yeast two-hybrid analysis identified putative Zat7 and EAR-domain interactors, including WRKY70 and HASTY.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- WRKY70 and HASTYe3
- Outcome
- putative interactors of Zat7 and the EAR-domaine3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic Arabidopsis plants constitutively expressing the Cys2/His2 zinc finger protein Zat7”
e3“A yeast two-hybrid analysis identified putative interactors of Zat7 and the EAR-domain, including WRKY70 and HASTY”
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