Paper · 2005 · Journal of Agricultural and Food Chemistry

Biodegradation of Oxalic Acid from Spinach Using Cereal Radicles

Thomas Betsche; Barbara Fretzdorff

6
claims
11
verbatim quotes
20
citations
1
research areas
—
Not open access

Abstract

A high level of oxalate intake constitutes a health risk for infants and metabolically disposed adults. Spinach, acclaimed for its many health benefits, is among the vegetables richest in oxalate. Blanching reduces oxalate unsatisfactorily and unspecifically. An alternative, biological method is proposed on the basis of rye seedlings or radicles (also barley and wheat) containing an oxalate-specific oxalate oxidase by nature. Dissolved oxalate (0.25 mM) was rapidly degraded in the presence of radicles (e.g., 70% within 100 min). With commercial deep-frozen spinach, near-complete degradation of soluble oxalate was achieved at pH 3.5. The total level of oxalate was reduced by half. Similarly high rates occurred from 18 to 35 degrees C. Even at 55 degrees C appreciable rates were observed. The seedling as a whole is effective, too, and enrichment with cereal-specific healthy components would occur. Removal of oxalate from other vegetables, juices, cycled process waters, or feeds is conceivable with fresh or heat-dried cereal seedlings or radicles.

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Claims 6

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Dissolved oxalate was rapidly degraded in the presence of radicles, about 70% within 100 min.

Organ / stage
radiclese1
Treatment
presence of radiclese1
Outcome
Dissolved oxalatee1
Direction
degradede1
Value
70% within 100 mine1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Secale cereale
Secale cereale · TaxonLSDSL · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Dissolved oxalate (0.25 mM) was rapidly degraded in the presence of radicles (e.g., 70% within 100 min)”

✓ located verbatim in the source · abstract, characters 430–533 · show in text ↑
e2

“on the basis of rye seedlings or radicles (also barley and wheat)”

✓ located verbatim in the source · abstract, characters 306–371 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Near-complete degradation of soluble oxalate was achieved in commercial deep-frozen spinach at pH 3.5.

Material
commercial deep-frozen spinache3
Treatment
pH 3.5e3
Outcome
soluble oxalatee3
Direction
near-complete degradatione3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e3

“With commercial deep-frozen spinach, near-complete degradation of soluble oxalate was achieved at pH 3.5”

✓ located verbatim in the source · abstract, characters 535–639 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The total level of oxalate in spinach was reduced by half.

Outcome
total level of oxalatee4
Direction
reducede4
Value
by halfe4

Not reportedTreatmentComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e4

“The total level of oxalate was reduced by half”

✓ located verbatim in the source · abstract, characters 641–687 · show in text ↑
e5

“With commercial deep-frozen spinach, near-complete degradation of soluble oxalate was achieved at pH 3.5”

✓ located verbatim in the source · abstract, characters 535–639 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

High oxalate degradation rates occurred from 18 to 35 degrees C.

Treatment
18 to 35 degrees Ce6
Outcome
ratese6
Direction
highe6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Secale cereale
Secale cereale · TaxonLSDSL · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e6

“Similarly high rates occurred from 18 to 35 degrees C”

✓ located verbatim in the source · abstract, characters 689–742 · show in text ↑
e2

“on the basis of rye seedlings or radicles (also barley and wheat)”

✓ located verbatim in the source · abstract, characters 306–371 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Appreciable oxalate degradation rates were observed at 55 degrees C.

Treatment
55 degrees Ce7
Outcome
ratese7

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Secale cereale
Secale cereale · TaxonLSDSL · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e7

“Even at 55 degrees C appreciable rates were observed”

✓ located verbatim in the source · abstract, characters 744–796 · show in text ↑
e2

“on the basis of rye seedlings or radicles (also barley and wheat)”

✓ located verbatim in the source · abstract, characters 306–371 · show in text ↑
Observationauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

The rye seedling as a whole is effective for oxalate degradation.

Outcome
effectivee3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Secale cereale
Secale cereale · TaxonLSDSL · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e3

“The seedling as a whole is effective, too”

✓ located verbatim in the source · abstract, characters 798–839 · show in text ↑
e2

“on the basis of rye seedlings or radicles (also barley and wheat)”

✓ located verbatim in the source · abstract, characters 306–371 · show in text ↑

ph · molecular: metabolites · Spinacia oleracea (spinach)Positive and negative results

7 papers: 3 positive, 4 negative or no effect

2 papers in the same direction as this paper · 4 in the opposite direction

  1. negative
  2. negative
  3. negative

3 papers more in this group

See all 7 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.