Abstract
The responses of steady-state cell populations of Acer pseudoplatanus L. (sycamore) in chemo-stats to changes in the nitrate concentration of their environment was generally predictable by equations derived for ideal, free-cell, nitrate-limited populations. A step-down in nitrate concentration in the input medium of a chemostat produced interlocking oscillations in spent-medium nitrate, intracellular pools of nitrate and amino-N, protein accumulation, and cell division. The rate of nitrate uptake into the cell and thus the flow of nitrogen into protein (and ultimately the specific growth rate) was seen to be finely regulated by spent-medium nitrate concentration. Steady states of growth of sycamore cells were established with glucose as the limiting nutrient both from inoculation and after switching from nitrate to glucose limitation. Glucose-limited cells produced by glucose step-down of a nitrate-limited population suffered major losses of cell material, including starch and cell wall polysaccharide, to the extent that protein then accounted for c. 70% of cell dry weight.
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Claims 5
Claim · model-written summary
A step-down in nitrate concentration produced interlocking oscillations in spent-medium nitrate in sycamore cell chemostats.
- Taxon
- Acer pseudoplatanus
Acer pseudoplatanuse0 - Treatment
- step-down in nitrate concentration in the input mediume1
- Outcome
- interlocking oscillations in spent-medium nitratee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Acer pseudoplatanus L. (sycamore)”
e1“A step-down in nitrate concentration in the input medium of a chemostat produced interlocking oscillations in spent-medium nitrate, intracellular pools of nitrate and amino-N, protein accumulation, and cell division”
Claim · model-written summary
The rate of nitrate uptake in sycamore cells is finely regulated by spent-medium nitrate concentration.
- Taxon
- Acer pseudoplatanusAcer pseudoplatanuse0
- Compound
- nitratee2
- Outcome
- rate of nitrate uptakee2
- Qualifier
- finely regulatede2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Acer pseudoplatanus L. (sycamore)”
e2“The rate of nitrate uptake into the cell and thus the flow of nitrogen into protein (and ultimately the specific growth rate) was seen to be finely regulated by spent-medium nitrate concentration”
Claim · model-written summary
Steady states of sycamore cell growth were established with glucose as the limiting nutrient.
- Taxon
- Acer pseudoplatanusAcer pseudoplatanuse0
- Treatment
- glucose as the limiting nutriente3
- Outcome
- Steady states of growthe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Acer pseudoplatanus L. (sycamore)”
e3“Steady states of growth of sycamore cells were established with glucose as the limiting nutrient both from inoculation and after switching from nitrate to glucose limitation”
Claim · model-written summary
Glucose step-down of a nitrate-limited population caused major losses of sycamore cell material.
- Taxon
- Acer pseudoplatanusAcer pseudoplatanuse0
- Treatment
- glucose step-downe4
- Outcome
- losses of cell materiale4
- Direction
- decreaseinferrede4
- Comparator
- nitrate-limited populatione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Acer pseudoplatanus L. (sycamore)”
e4“Glucose-limited cells produced by glucose step-down of a nitrate-limited population suffered major losses of cell material, including starch and cell wall polysaccharide, to the extent that protein then accounted for c. 70% of cell dry weight”
Claim · model-written summary
Protein accounted for about 70% of the dry weight of glucose-limited sycamore cells.
- Taxon
- Acer pseudoplatanusAcer pseudoplatanuse0
- Outcome
- proteine4
- Value
- c. 70%e4
- Qualifier
- of cell dry weighte4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Acer pseudoplatanus L. (sycamore)”
e4“Glucose-limited cells produced by glucose step-down of a nitrate-limited population suffered major losses of cell material, including starch and cell wall polysaccharide, to the extent that protein then accounted for c. 70% of cell dry weight”