Abstract
In plant cells the plasma membrane is a highly elaborated structure that functions as the point of exchange with adjoining cells, cell walls and the external environment. In this study, we investigated the structure and function characteristic of wheat root plasma membrane (PM) as affected by H2O2 and Fe by using fluorescence spectroscopic and attenuated total reflectance infrared (ATR-IR) techniques. The results showed that these oxidant damaged induced an obviously reduced membrane fluidity were observed in the roots PM treated with the 200 μM H2O2, FeSO4, and FeCl3. Computer-aided software analyses of the FTIR spectrum indicated that the content of the α-helices decreased and β-sheet increased in the secondary structures of proteins after exposure to the oxidants of 200 μM H2O2, FeSO4, and FeCl3. The number of P=O and C=C bonds area declined rapidly in the lipids of the membrane under the oxidants stress. These structural alterations might explain the reason of the roots PM instability under most of the abiotic stress.
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Claims 5
Claim · model-written summary
H2O2, FeSO4 and FeCl3 reduced wheat root membrane fluidity.
- Taxon
- wheat
Triticum aestivume1 - Organ / stage
- roots PMe0
- Treatment
- 200 μM H2O2, FeSO4, and FeCl3e0
- Outcome
- membrane fluiditye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reduced membrane fluidity were observed in the roots PM treated with the 200 μM H2O2, FeSO4, and FeCl3”
e1“wheat root plasma membrane (PM)”
Claim · model-written summary
Oxidant exposure decreased membrane protein α-helices.
- Taxon
- wheatTriticum aestivume1
- Treatment
- 200 μM H2O2, FeSO4, and FeCl3e0
- Outcome
- content of the α-helicese0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the content of the α-helices decreased and β-sheet increased in the secondary structures of proteins after exposure to the oxidants of 200 μM H2O2, FeSO4, and FeCl3”
e1“wheat root plasma membrane (PM)”
Claim · model-written summary
Oxidant exposure increased membrane protein β-sheet content.
- Taxon
- wheatTriticum aestivume1
- Treatment
- 200 μM H2O2, FeSO4, and FeCl3e0
- Outcome
- β-sheete0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“β-sheet increased in the secondary structures of proteins after exposure to the oxidants of 200 μM H2O2, FeSO4, and FeCl3”
e1“wheat root plasma membrane (PM)”
Claim · model-written summary
Oxidant stress rapidly reduced P=O and C=C bond area in membrane lipids.
- Taxon
- wheatTriticum aestivume1
- Treatment
- oxidants stresse0
- Outcome
- number of P=O and C=C bonds areae0
- Direction
- declined rapidlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The number of P=O and C=C bonds area declined rapidly in the lipids of the membrane under the oxidants stress”
e1“wheat root plasma membrane (PM)”
Claim · model-written summary
The authors suggest structural changes explain root plasma membrane instability under abiotic stress.
- Taxon
- wheatTriticum aestivume1
- Outcome
- roots PM instabilitye0
- Qualifier
- structural alterationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These structural alterations might explain the reason of the roots PM instability under most of the abiotic stress”
e1“wheat root plasma membrane (PM)”
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