Abstract
Symptoms typical of senescence occurred in green detached primary barley (Hordeum vulgare L.) leaves placed in darkness and in light. Chlorophyll, total soluble protein, ribulose 1,5-diphosphate carboxylase protein and activity each progressively decreased in darkness and to a lesser extent in light. In all treatments most of the total soluble protein lost was accounted for by a decrease in ribulose 1,5-diphosphate carboxylase protein, suggesting that the chloroplast was a major site of degradation early in senescence.Loss of ribulose 1,5-diphosphate carboxylase protein was negatively correlated with an increase in proteolytic activity measured against azocasein. Both rates were exponential, with about a 30% difference in apparent rate constants. Cycloheximide essentially prevented the loss of chlorophyll, ribulose 1,5-diphosphate carboxylase protein, and activity and completely inhibited the increase in proteolytic activity against azocasein. Since chloramphenicol had little effect on the loss of ribulose 1,5-diphosphate carboxylase protein or chlorophyll, or on proteolytic activity against azocasein, it is suggested that the proteolytic activity was developed on cytoplasmic 80 S ribosomes.Kinetin greatly retarded the onset of such symptoms of senescence by inhibiting the losses of chlorophyll and ribulose 1,5-diphosphate carboxylase protein and protected against inactivation of enzymic activity. It also prevented the increase in proteolytic activity measured against azocasein. Incorporation of labeled amino acids into ribulose 1,5-diphosphate carboxylase during its rapid degradation showed that the enzyme was under turnover. The changes in ribulose 1,5-diphosphate carboxylase protein and activity, chlorophyll, soluble protein other than ribulose 1,5-diphosphate carboxylase, proteolytic and esterolytic activity during senescence indicate that senescence is a selective, sequential process.
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Claims 15
Claim · model-written summary
Detached barley leaves developed senescence symptoms in darkness and light.
- Taxon
- barley (Hordeum vulgare L.)
.jpg?width=60)
Hordeum vulgaree0 - Organ / stage
- detached primary barley (Hordeum vulgare L.) leavese0
- Outcome
- Symptoms typical of senescencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Symptoms typical of senescence occurred in green detached primary barley (Hordeum vulgare L.) leaves placed in darkness and in light”
Claim · model-written summary
Chlorophyll and total soluble protein decreased in darkness, less so in light.
- Taxon
- barley (Hordeum vulgare L.)Hordeum vulgaree1
- Treatment
- darknesse0
- Outcome
- Chlorophyll, total soluble proteine0
- Direction
- decreasede0
- Comparator
- lighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chlorophyll, total soluble protein, ribulose 1,5-diphosphate carboxylase protein and activity each progressively decreased in darkness and to a lesser extent in light”
e1“Symptoms typical of senescence occurred in green detached primary barley (Hordeum vulgare L.) leaves placed in darkness and in light”
Claim · model-written summary
Rubisco protein and activity progressively decreased in darkness, less so in light.
- Taxon
- barley (Hordeum vulgare L.)Hordeum vulgaree1
- Treatment
- darknesse0
- Gene
- ribulose 1,5-diphosphate carboxylasee0
- Outcome
- ribulose 1,5-diphosphate carboxylase protein and activitye0
- Direction
- decreasede0
- Comparator
- lighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chlorophyll, total soluble protein, ribulose 1,5-diphosphate carboxylase protein and activity each progressively decreased in darkness and to a lesser extent in light”
e1“Symptoms typical of senescence occurred in green detached primary barley (Hordeum vulgare L.) leaves placed in darkness and in light”
Claim · model-written summary
Most soluble protein loss was attributable to rubisco loss.
- Taxon
- barley (Hordeum vulgare L.)Hordeum vulgaree1
- Gene
- ribulose 1,5-diphosphate carboxylasee0
- Outcome
- total soluble protein loste0
- Direction
- decreasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In all treatments most of the total soluble protein lost was accounted for by a decrease in ribulose 1,5-diphosphate carboxylase protein”
e1“Symptoms typical of senescence occurred in green detached primary barley (Hordeum vulgare L.) leaves placed in darkness and in light”
Claim · model-written summary
Rubisco protein loss was negatively correlated with proteolytic activity increase.
- Taxon
- barley (Hordeum vulgare L.)Hordeum vulgaree1
- Gene
- ribulose 1,5-diphosphate carboxylasee0
- Outcome
- proteolytic activity measured against azocaseine0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Loss of ribulose 1,5-diphosphate carboxylase protein was negatively correlated with an increase in proteolytic activity measured against azocasein”
t0“barley”
e1“Symptoms typical of senescence occurred in green detached primary barley (Hordeum vulgare L.) leaves placed in darkness and in light”
Claim · model-written summary
Both rates were exponential and apparent rate constants differed about 30%.
- Taxon
- barley (Hordeum vulgare L.)Hordeum vulgaree1
- Outcome
- apparent rate constantse0
- Direction
- differencee0
- Value
- 30%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both rates were exponential, with about a 30% difference in apparent rate constants”
t0“barley”
e1“Symptoms typical of senescence occurred in green detached primary barley (Hordeum vulgare L.) leaves placed in darkness and in light”
Claim · model-written summary
Cycloheximide prevented chlorophyll and rubisco protein and activity loss.
- Taxon
- barley (Hordeum vulgare L.)Hordeum vulgaree1
- Treatment
- Cycloheximidee0
- Outcome
- loss of chlorophyll, ribulose 1,5-diphosphate carboxylase protein, and activitye0
- Direction
- preventede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cycloheximide essentially prevented the loss of chlorophyll, ribulose 1,5-diphosphate carboxylase protein, and activity”
t0“barley”
e1“Symptoms typical of senescence occurred in green detached primary barley (Hordeum vulgare L.) leaves placed in darkness and in light”
Claim · model-written summary
Cycloheximide inhibited proteolytic activity increase.
- Taxon
- barley (Hordeum vulgare L.)Hordeum vulgaree1
- Treatment
- Cycloheximidee0
- Outcome
- proteolytic activity against azocaseine0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cycloheximide essentially prevented the loss of chlorophyll, ribulose 1,5-diphosphate carboxylase protein, and activity and completely inhibited the increase in proteolytic activity against azocasein”
t0“barley”
e1“Symptoms typical of senescence occurred in green detached primary barley (Hordeum vulgare L.) leaves placed in darkness and in light”
Claim · model-written summary
Chloramphenicol had little effect on rubisco protein or chlorophyll loss or proteolytic activity.
- Taxon
- barley (Hordeum vulgare L.)Hordeum vulgaree1
- Treatment
- chloramphenicole0
- Outcome
- loss of ribulose 1,5-diphosphate carboxylase protein or chlorophyll, or on proteolytic activitye0
- Direction
- little effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“chloramphenicol had little effect on the loss of ribulose 1,5-diphosphate carboxylase protein or chlorophyll, or on proteolytic activity against azocasein”
t0“barley”
e1“Symptoms typical of senescence occurred in green detached primary barley (Hordeum vulgare L.) leaves placed in darkness and in light”
Claim · model-written summary
Authors suggest proteolytic activity developed on cytoplasmic 80 S ribosomes.
- Taxon
- barley (Hordeum vulgare L.)Hordeum vulgaree1
- Outcome
- proteolytic activitye0
- Qualifier
- cytoplasmic 80 S ribosomese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it is suggested that the proteolytic activity was developed on cytoplasmic 80 S ribosomes”
t0“barley”
e1“Symptoms typical of senescence occurred in green detached primary barley (Hordeum vulgare L.) leaves placed in darkness and in light”
Claim · model-written summary
Kinetin retarded onset of senescence symptoms.
- Taxon
- barley (Hordeum vulgare L.)Hordeum vulgaree1
- Treatment
- Kinetine0
- Outcome
- onset of such symptoms of senescencee0
- Direction
- retardede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Kinetin greatly retarded the onset of such symptoms of senescence”
t0“barley”
e1“Symptoms typical of senescence occurred in green detached primary barley (Hordeum vulgare L.) leaves placed in darkness and in light”
Claim · model-written summary
Kinetin inhibited loss of chlorophyll and rubisco protein and protected enzyme activity.
- Taxon
- barley (Hordeum vulgare L.)Hordeum vulgaree1
- Treatment
- Kinetine0
- Outcome
- losses of chlorophyll and ribulose 1,5-diphosphate carboxylase proteine0
- Direction
- inhibitinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Kinetin greatly retarded the onset of such symptoms of senescence by inhibiting the losses of chlorophyll and ribulose 1,5-diphosphate carboxylase protein and protected against inactivation of enzymic activity”
t0“barley”
e1“Symptoms typical of senescence occurred in green detached primary barley (Hordeum vulgare L.) leaves placed in darkness and in light”
Claim · model-written summary
Kinetin prevented increased proteolytic activity.
- Taxon
- barley (Hordeum vulgare L.)Hordeum vulgaree1
- Treatment
- Kinetine2
- Outcome
- proteolytic activity measured against azocaseine0
- Direction
- preventede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It also prevented the increase in proteolytic activity measured against azocasein”
t0“barley”
e1“Symptoms typical of senescence occurred in green detached primary barley (Hordeum vulgare L.) leaves placed in darkness and in light”
e2“Kinetin greatly retarded the onset of such symptoms of senescence”
Claim · model-written summary
Rubisco underwent turnover during rapid degradation.
- Taxon
- barley (Hordeum vulgare L.)Hordeum vulgaree1
- Gene
- ribulose 1,5-diphosphate carboxylasee0
- Outcome
- enzyme was under turnovere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Incorporation of labeled amino acids into ribulose 1,5-diphosphate carboxylase during its rapid degradation showed that the enzyme was under turnover”
t0“barley”
e1“Symptoms typical of senescence occurred in green detached primary barley (Hordeum vulgare L.) leaves placed in darkness and in light”
Claim · model-written summary
Authors interpret senescence as a selective sequential process.
- Taxon
- barley (Hordeum vulgare L.)Hordeum vulgaree1
- Outcome
- senescence is a selective, sequential processe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“senescence is a selective, sequential process”
t0“barley”
e1“Symptoms typical of senescence occurred in green detached primary barley (Hordeum vulgare L.) leaves placed in darkness and in light”
The same questions in other papers
kinetin · physiology: pigments · Hordeum vulgare (barley)Results differ
4 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
2 papers more in this group
See all 6 papers →chloramphenicol · physiology: pigments · Hordeum vulgare (barley)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
cycloheximide · physiology: pigments · Hordeum vulgare (barley)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
kinetin · disease · Hordeum vulgare (barley)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.