Abstract
BACKGROUND: The SR/CAMTA proteins represent a small family of transcription activators that play important roles in plant responses to biotic and abiotic stresses. Seven SlSR/CAMTA genes were identified in tomato as tomato counterparts of SR/CAMTA; however, the involvement of SlSRs/CAMTAs in biotic and abiotic stress responses is not clear. In this study, we performed functional analysis of the SlSR/CAMTA family for their possible functions in defense response against pathogens and tolerance to drought stress. RESULTS: Expression of SlSRs was induced with distinct patterns by Botrytis cinerea and Pseudomonas syringae pv. tomato (Pst) DC3000. Virus-induced gene silencing (VIGS)-based knockdown of either SlSR1 or SlSR3L in tomato resulted in enhanced resistance to B. cinerea and Pst DC3000 and led to constitutive accumulation of H2O2, elevated expression of defense genes, marker genes for pathogen-associated molecular pattern-triggered immunity, and regulatory genes involved in the salicylic acid- and ethylene-mediated signaling pathways. Furthermore, the expression of SlSR1L and SlSR2L in detached leaves and whole plants was significantly induced by drought stress. Silencing of SlSR1L led to decreased drought stress tolerance, accelerated water loss in leaves, reduced root biomass and attenuated expression of drought stress responsive genes in tomato. The SlSR1 and SlSR3L proteins were localized in the nucleus of plant cells when transiently expressed in Nicotiana benthamiana and had transcriptional activation activity in yeast. CONCLUSIONS: VIGS-based functional analyses demonstrate that both SlSR1 and SlSR3L in the tomato SlSR/CAMTA family are negative regulators of defense response against B. cinerea and Pst DC3000 while SlSR1L is a positive regulator of drought stress tolerance in tomato.
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Claims 12
Claim · model-written summary
Botrytis cinerea induced distinct SlSR expression patterns.
- Taxon
- Botrytis cinerea
Botrytis cinereae0 - Outcome
- Expression of SlSRse0
- Direction
- inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of SlSRs was induced with distinct patterns by Botrytis cinerea and Pseudomonas syringae pv. tomato (Pst) DC3000.”
Claim · model-written summary
Pseudomonas syringae pv. tomato DC3000 induced distinct SlSR expression patterns.
- Taxon
- Pseudomonas syringae pv. tomato (Pst) DC3000
Pseudomonas syringae pv. tomatoe0 - Outcome
- Expression of SlSRse0
- Direction
- inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of SlSRs was induced with distinct patterns by Botrytis cinerea and Pseudomonas syringae pv. tomato (Pst) DC3000.”
Claim · model-written summary
Silencing SlSR1 enhanced tomato resistance to B. cinerea and Pst DC3000.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- knockdown of either SlSR1 or SlSR3Le0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Gene
- SlSR1e0
- Outcome
- resistance to B. cinerea and Pst DC3000e0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Virus-induced gene silencing (VIGS)-based knockdown of either SlSR1 or SlSR3L in tomato resulted in enhanced resistance to B. cinerea and Pst DC3000”
Claim · model-written summary
Silencing SlSR3L enhanced tomato resistance to B. cinerea and Pst DC3000.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- knockdown of either SlSR1 or SlSR3Le0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Gene
- SlSR3Le0
- Outcome
- resistance to B. cinerea and Pst DC3000e0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“knockdown of either SlSR1 or SlSR3L in tomato resulted in enhanced resistance to B. cinerea and Pst DC3000”
Claim · model-written summary
Silencing SlSR1 or SlSR3L caused constitutive H2O2 accumulation and elevated defense gene expression.
Not reportedTaxon
Verbatim
e0“led to constitutive accumulation of H2O2, elevated expression of defense genes, marker genes for pathogen-associated molecular pattern-triggered immunity, and regulatory genes involved in the salicylic acid- and ethylene-mediated signaling pathways.”
Claim · model-written summary
Drought significantly induced SlSR1L and SlSR2L expression.
Not reportedTaxon
Verbatim
e0“the expression of SlSR1L and SlSR2L in detached leaves and whole plants was significantly induced by drought stress.”
Claim · model-written summary
Silencing SlSR1L decreased tomato drought tolerance.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- Silencing of SlSR1Le0
- Gene
- SlSR1Le0
- Outcome
- drought stress tolerancee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of SlSR1L led to decreased drought stress tolerance, accelerated water loss in leaves, reduced root biomass and attenuated expression of drought stress responsive genes in tomato.”
Claim · model-written summary
Silencing SlSR1L accelerated tomato leaf water loss.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- Silencing of SlSR1Le0
- Outcome
- water loss in leavese0
- Direction
- acceleratede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of SlSR1L led to decreased drought stress tolerance, accelerated water loss in leaves, reduced root biomass and attenuated expression of drought stress responsive genes in tomato.”
Claim · model-written summary
Silencing SlSR1L reduced tomato root biomass.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- Silencing of SlSR1Le0
- Outcome
- root biomasse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of SlSR1L led to decreased drought stress tolerance, accelerated water loss in leaves, reduced root biomass and attenuated expression of drought stress responsive genes in tomato.”
Claim · model-written summary
Silencing SlSR1L attenuated tomato drought responsive gene expression.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- Silencing of SlSR1Le0
- Outcome
- expression of drought stress responsive genese0
- Direction
- attenuatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of SlSR1L led to decreased drought stress tolerance, accelerated water loss in leaves, reduced root biomass and attenuated expression of drought stress responsive genes in tomato.”
Claim · model-written summary
SlSR1 and SlSR3L localized to the nucleus when transiently expressed in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Outcome
- localized in the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The SlSR1 and SlSR3L proteins were localized in the nucleus of plant cells when transiently expressed in Nicotiana benthamiana”
Claim · model-written summary
SlSR1 and SlSR3L had transcriptional activation activity in yeast.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Outcome
- transcriptional activation activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlSR1 and SlSR3L proteins were localized in the nucleus of plant cells when transiently expressed in Nicotiana benthamiana and had transcriptional activation activity in yeast.”
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