Abstract
Abstract The composition and rationale of a broad spectrum tissue culture experiment involving 81 different media are described. Tobacco calluses sub‐cultured on media in this experiment were induced to form six main types of callus depending on the concentration levels of minerals, auxins, cytokinins, and sucrose, growth factors and amino acids in the medium. Only nine of the 81 media inhibited callus growth, and growth (increase in mass divided by initial mass of fresh matter) varied considerably reaching a maximum of nearly 200 after eight weeks on a medium high in minerals, cytokinins, sucrose, growth factors and amino acids and low in auxins. Five media induced regeneration from the calluses. This experiment is suggested as a potentially fruitful introductory test for many new or unresolved tissue culture situations.
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Claims 5
Claim · model-written summary
Tobacco calluses formed six main callus types depending on medium composition.
- Taxon
- Tobacco
Nicotiana tabacumt0 - Organ / stage
- calluse0
- Treatment
- mediae0
- Outcome
- six main types of calluse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco (Nicotiana tabacum)”
e0“Tobacco calluses sub‐cultured on media in this experiment were induced to form six main types of callus depending on the concentration levels of minerals, auxins, cytokinins, and sucrose, growth factors and amino acids in the medium”
Claim · model-written summary
Nine of 81 media inhibited tobacco callus growth.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- nine of the 81 mediae0
- Outcome
- callus growthe0
- Direction
- inhibitede0
- Value
- nine of the 81 mediae0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco (Nicotiana tabacum)”
e0“Only nine of the 81 media inhibited callus growth”
Claim · model-written summary
Tobacco callus growth reached nearly 200 after eight weeks on nutrient-rich, low-auxin medium.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- a medium high in minerals, cytokinins, sucrose, growth factors and amino acids and low in auxinse0
- Outcome
- growthe0
- Direction
- maximume0
- Value
- nearly 200e0
- Qualifier
- after eight weekse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco (Nicotiana tabacum)”
e0“growth (increase in mass divided by initial mass of fresh matter) varied considerably reaching a maximum of nearly 200 after eight weeks on a medium high in minerals, cytokinins, sucrose, growth factors and amino acids and low in auxins”
Claim · model-written summary
Five media induced tobacco callus regeneration.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- Five mediae0
- Outcome
- regeneration from the callusese0
- Value
- Fivee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco (Nicotiana tabacum)”
e0“Five media induced regeneration from the calluses”
Claim · model-written summary
The authors suggested the broad spectrum culture experiment as an introductory test for new tissue culture situations.
- Taxon
- TobaccoNicotiana tabacumt0
- Outcome
- potentially fruitful introductory teste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This experiment is suggested as a potentially fruitful introductory test for many new or unresolved tissue culture situations”
t0“Tobacco (Nicotiana tabacum)”
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See all 40 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.