Abstract
Phaseolus vulgaris (L) is poorly nodulated in all the regions of Tunisia where this crop is grown.Selection of effective strains needs to be developed.An effectiveness test trial was carried out on Royalnel and Coco cultivars in hydroponic aseptic conditions for seven reference strains and three local isolates of common bean rhizobia.A significant interaction strain × cultivar was observed and, unlike local isolates, most reference strains were very effective.The ELISA technique using polyclonal antibody was used to evaluate nodule occupancy of inoculated common bean rhi- zobia in Tunisian soils.Rabbit antisera were raised against one local isolate (HB) and five reference strains (CIAT899, USDA2667, USDA2669, USDA2676 and IRATYH112).Analysis of relatedness between 44 strains and isolates showed that 77% of the local isolates did not react with any of the sera used, whereas the remaining isolates were dis- tributed into three serogroups.An inoculation experiment with the selected reference strains CIAT899, USDA2667 and IRATYH112, which are serologically distinguishable from native rhizobia, was performed in a green house for Royalnel and Coco cultivars grown in soil cores from two sites (Boucharraï and Chrifet).Nodule occupancy of the inoc- ulated strains was assessed and concomitant effect of inoculation on nodule number and shoot nitrogen content was analysed.A highly significant treatment x cultivar x site interaction was observed for the parameters studied.While in Boucharraï soil inoculation leads to a significant improvement of shoot nitrogen content for Royalnel cultivar, inocu- lation of the same cultivar in Chrifet soil leads to an increase in nodulation by native rhizobia without improvement of plant growth.For the two cultivars, N-fertilization leads for the two cultivars to a decrease in nodule number, particularly in Chrifet soil.Thus, to improve establishment of symbiotic process, the use of N-fertilizer should be rationalized.inoculation / rhizobia / Phaseolus vulgaris / ELISA Résumé -Faisabilité d'inoculation du haricot par des Rhizobium efficients en sols tunisiens.Le haricot (Phaseolus *Correspondence and reprints et Chrifet).Le pourcentage de nodosités formés par les souches inoculées a été estimé et l'effet de l'inoculation sur le nombre de nodules et l'azote dans les parties aériennes ont été analysés.Une interaction hautement significative traite- ment x cultivar x site a été observée pour les paramètres étudiés.Alors que sur le sol de Boucharraï l'inoculation conduit à une amélioration significative du contenu en azote des parties aériennes pour le cultivar Royalnel, l'inocula- tion du même cultivar sur sol de Chrifet conduit plutôt à une stimulation de la nodulation par les bactéries natives sans amélioration de la croissance végétale.L'application d'engrais azoté conduit pour les deux cultivars à une diminution de la nodulation particulièrement sur sol de Chrifet.En vue de favoriser l'établissement du processus symbiotique, l'uti- lisation de ce composé doit être rationalisée.inoculation / Rhizobium / Phaseolus vulgaris / Elisa
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Claims 10
Claim · model-written summary
Strain effectiveness depended significantly on cultivar.
- Taxon
- common bean
Phaseolus vulgarist0 - Outcome
- interaction strain × cultivare0
Not reportedMaterialDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A significant interaction strain × cultivar was observed”
t0“common bean”
Claim · model-written summary
Most reference strains were highly effective compared with local isolates.
- Taxon
- common beanPhaseolus vulgarist0
- Outcome
- most reference strains were very effectivee0
- Comparator
- local isolatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“unlike local isolates, most reference strains were very effective.”
t0“common bean”
Claim · model-written summary
Seventy-seven percent of local isolates failed to react with the tested sera.
- Taxon
- common beanPhaseolus vulgarist0
- Outcome
- local isolates did not react with any of the sera usede0
- Value
- 77%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“77% of the local isolates did not react with any of the sera used”
t0“common bean”
Claim · model-written summary
The remaining isolates fell into three serogroups.
- Taxon
- common beanPhaseolus vulgarist0
- Outcome
- remaining isolates were dis- tributed into three serogroupse0
- Value
- three serogroupse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the remaining isolates were dis- tributed into three serogroups.”
t0“common bean”
Claim · model-written summary
Treatment, cultivar, and site interacted significantly for studied nodulation and nitrogen measures.
- Taxon
- common beanPhaseolus vulgarist0
- Outcome
- treatment x cultivar x site interactione0
- Setting
- sitee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A highly significant treatment x cultivar x site interaction was observed for the parameters studied.”
t0“common bean”
Claim · model-written summary
Inoculation increased Royalnel shoot nitrogen in Boucharraï soil.
- Taxon
- common beanPhaseolus vulgarist0
- Material
- Royalnel cultivare0
- Treatment
- inoculatione0
- Outcome
- shoot nitrogen contente0
- Direction
- improvemente0
- Setting
- Boucharraï soile0
Not reportedInteractorComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in Boucharraï soil inoculation leads to a significant improvement of shoot nitrogen content for Royalnel cultivar”
t0“common bean”
Claim · model-written summary
Inoculation increased native-rhizobial nodulation of Royalnel in Chrifet soil.
- Taxon
- common beanPhaseolus vulgarist0
- Treatment
- inoculatione1
- Interactor
- native rhizobiae0symbiont
- Outcome
- nodulation by native rhizobiae0
- Direction
- increasee0
- Setting
- Chrifet soile0
Not reportedMaterialComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inocu- lation of the same cultivar in Chrifet soil leads to an increase in nodulation by native rhizobia without improvement of plant growth.”
t0“common bean”
e1“inoculation leads to a significant improvement of shoot nitrogen content for Royalnel cultivar”
Claim · model-written summary
Inoculation did not improve Royalnel plant growth in Chrifet soil.
- Taxon
- common beanPhaseolus vulgarist0
- Treatment
- inoculatione1
- Interactor
- native rhizobiae0symbiont
- Outcome
- plant growthe0
- Direction
- without improvemente0
- Setting
- Chrifet soile0
Not reportedMaterialComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inocu- lation of the same cultivar in Chrifet soil leads to an increase in nodulation by native rhizobia without improvement of plant growth.”
t0“common bean”
e1“inoculation leads to a significant improvement of shoot nitrogen content for Royalnel cultivar”
Claim · model-written summary
Nitrogen fertilization decreased nodule number in both cultivars, especially in Chrifet soil.
- Taxon
- common beanPhaseolus vulgarist0
- Treatment
- N-fertilizatione0
- Outcome
- nodule numbere0
- Direction
- decreasee0
- Setting
- Chrifet soile0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“For the two cultivars, N-fertilization leads for the two cultivars to a decrease in nodule number, particularly in Chrifet soil.”
t0“common bean”
Claim · model-written summary
The authors recommend rationalizing nitrogen fertilizer use to improve symbiosis.
- Taxon
- common beanPhaseolus vulgarist0
- Outcome
- the use of N-fertilizer should be rationalizede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“to improve establishment of symbiotic process, the use of N-fertilizer should be rationalized.”
t0“common bean”
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