Abstract
Tm-2² is a coiled coil-nucleotide binding-leucine rich repeat resistance protein that confers durable extreme resistance against Tomato mosaic virus (ToMV) and Tobacco mosaic virus (TMV) by recognizing the viral movement protein (MP). Here we report that the Nicotiana benthamiana J-domain MIP1 proteins (NbMIP1s) associate with tobamovirus MP, Tm-2² and SGT1. Silencing of NbMIP1s reduced TMV movement and compromised Tm-2²-mediated resistance against TMV and ToMV. Furthermore, silencing of NbMIP1s reduced the steady-state protein levels of ToMV MP and Tm-2². Moreover, NbMIP1s are required for plant resistance induced by other R genes and the nonhost pathogen Pseudomonas syringae pv. tomato (Pst) DC3000. In addition, we found that SGT1 associates with Tm-2² and is required for Tm-2²-mediated resistance against TMV. These results suggest that NbMIP1s function as co-chaperones during virus infection and plant immunity.
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Claims 8
Claim · model-written summary
NbMIP1s associate with tobamovirus MP, Tm-2² and SGT1 in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae1 - Interactor
- tobamovirus
Tobamoviruse1pathogen - Gene
- NbMIP1se1
- Outcome
- associate with tobamovirus MP, Tm-2² and SGT1e1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here we report that the Nicotiana benthamiana J-domain MIP1 proteins (NbMIP1s) associate with tobamovirus MP, Tm-2² and SGT1”
Claim · model-written summary
Silencing of NbMIP1s reduced TMV movement in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- Silencing of NbMIP1se2
- Interactor
- TMV
Tobacco mosaic viruse2pathogen - Gene
- NbMIP1se2
- Outcome
- TMV movemente2
- Direction
- reducede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here we report that the Nicotiana benthamiana J-domain MIP1 proteins (NbMIP1s) associate with tobamovirus MP, Tm-2² and SGT1”
e2“Silencing of NbMIP1s reduced TMV movement and compromised Tm-2²-mediated resistance against TMV and ToMV”
Claim · model-written summary
Silencing of NbMIP1s compromised Tm-2²-mediated resistance against TMV and ToMV in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- Silencing of NbMIP1se2
- Interactor
- TMVTobacco mosaic viruse2pathogen
- Second interactor
- ToMVe2pathogen
- Gene
- Tm-2²e2
- Outcome
- Tm-2²-mediated resistance against TMVe2
- Direction
- compromisede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here we report that the Nicotiana benthamiana J-domain MIP1 proteins (NbMIP1s) associate with tobamovirus MP, Tm-2² and SGT1”
e2“Silencing of NbMIP1s reduced TMV movement and compromised Tm-2²-mediated resistance against TMV and ToMV”
Claim · model-written summary
Silencing of NbMIP1s reduced steady-state protein levels of ToMV MP and Tm-2² in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- silencing of NbMIP1se3
- Interactor
- ToMVe3pathogen
- Gene
- NbMIP1se3
- Outcome
- steady-state protein levelse3
- Direction
- reducede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here we report that the Nicotiana benthamiana J-domain MIP1 proteins (NbMIP1s) associate with tobamovirus MP, Tm-2² and SGT1”
e3“Furthermore, silencing of NbMIP1s reduced the steady-state protein levels of ToMV MP and Tm-2²”
Claim · model-written summary
NbMIP1s are required for plant resistance induced by other R genes and by Pseudomonas syringae pv. tomato DC3000.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Interactor
- Pseudomonas syringae pv. tomato (Pst) DC3000
Pseudomonas syringae pv. tomatoe4pathogen - Gene
- NbMIP1se4
- Outcome
- plant resistance induced by other R genese4
- Direction
- increaseinferrede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here we report that the Nicotiana benthamiana J-domain MIP1 proteins (NbMIP1s) associate with tobamovirus MP, Tm-2² and SGT1”
e4“Moreover, NbMIP1s are required for plant resistance induced by other R genes and the nonhost pathogen Pseudomonas syringae pv. tomato (Pst) DC3000”
Claim · model-written summary
SGT1 associates with Tm-2² in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Gene
- SGT1e5
- Outcome
- associates with Tm-2²e5
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here we report that the Nicotiana benthamiana J-domain MIP1 proteins (NbMIP1s) associate with tobamovirus MP, Tm-2² and SGT1”
e5“In addition, we found that SGT1 associates with Tm-2² and is required for Tm-2²-mediated resistance against TMV”
Claim · model-written summary
SGT1 is required for Tm-2²-mediated resistance against TMV in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Interactor
- TMVTobacco mosaic viruse5pathogen
- Gene
- SGT1e5
- Outcome
- Tm-2²-mediated resistance against TMVe5
- Direction
- increaseinferrede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here we report that the Nicotiana benthamiana J-domain MIP1 proteins (NbMIP1s) associate with tobamovirus MP, Tm-2² and SGT1”
e5“In addition, we found that SGT1 associates with Tm-2² and is required for Tm-2²-mediated resistance against TMV”
Claim · model-written summary
NbMIP1s may function as co-chaperones during virus infection and plant immunity.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Gene
- NbMIP1se6
- Outcome
- virus infection and plant immunitye6
- Qualifier
- co-chaperonese6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here we report that the Nicotiana benthamiana J-domain MIP1 proteins (NbMIP1s) associate with tobamovirus MP, Tm-2² and SGT1”
e6“These results suggest that NbMIP1s function as co-chaperones during virus infection and plant immunity”
The same questions in other papers
loss of function · Tobacco mosaic virus · Nicotiana benthamianaPositive and negative results
7 papers in the same direction as this paper · 13 in the opposite direction
- negative
- positive
- negative
17 papers more in this group
See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.