Abstract
Autophagy as a conserved catabolic pathway can respond to reactive oxygen species (ROS) and plays an important role in degrading oxidized proteins in plants under various stress conditions. However, how ROS regulates autophagy in response to oxidative stresses is largely unknown. Here, we show that autophagy-related protein 3 (ATG3) interacts with the cytosolic glyceraldehyde-3-phosphate dehydrogenases (GAPCs) to regulate autophagy in Nicotiana benthamiana plants. We found that oxidative stress inhibits the interaction of ATG3 with GAPCs. Silencing of GAPCs significantly activates ATG3-dependent autophagy, while overexpression of GAPCs suppresses autophagy in N. benthamiana plants. Moreover, silencing of GAPCs enhances N gene-mediated cell death and plant resistance against both incompatible pathogens Tobacco mosaic virus and Pseudomonas syringae pv tomato DC3000, as well as compatible pathogen P. syringae pv tabaci. These results indicate that GAPCs have multiple functions in the regulation of autophagy, hypersensitive response, and plant innate immunity.
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Claims 8
Claim · model-written summary
ATG3 interacted with GAPCs in plants.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Gene
- ATG3e0
- Outcome
- interacts with the cytosolic glyceraldehyde-3-phosphate dehydrogenases (GAPCs)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e0“autophagy-related protein 3 (ATG3) interacts with the cytosolic glyceraldehyde-3-phosphate dehydrogenases (GAPCs) to regulate autophagy in Nicotiana benthamiana plants”
Claim · model-written summary
Oxidative stress inhibited ATG3 interaction with GAPCs.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- oxidative stresse0
- Gene
- ATG3e0
- Outcome
- interaction of ATG3 with GAPCse0
- Direction
- inhibitse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e0“oxidative stress inhibits the interaction of ATG3 with GAPCs”
Claim · model-written summary
GAPC silencing significantly activated ATG3-dependent autophagy.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Silencing of GAPCse0
- Gene
- GAPCse0
- Outcome
- ATG3-dependent autophagye0
- Direction
- activatese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e0“Silencing of GAPCs significantly activates ATG3-dependent autophagy”
Claim · model-written summary
GAPC overexpression suppressed autophagy.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- overexpression of GAPCse0
- Gene
- GAPCse0
- Outcome
- autophagye0
- Direction
- suppressese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e0“overexpression of GAPCs suppresses autophagy in N. benthamiana plants”
Claim · model-written summary
GAPC silencing enhanced N gene-mediated cell death.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- silencing of GAPCse0
- Gene
- N genee0
- Outcome
- N gene-mediated cell deathe0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e0“silencing of GAPCs enhances N gene-mediated cell death”
Claim · model-written summary
GAPC silencing enhanced resistance to Tobacco mosaic virus.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- silencing of GAPCse0
- Interactor
- Tobacco mosaic virus
Tobacco mosaic viruse0pathogen - Outcome
- plant resistancee0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e0“silencing of GAPCs enhances N gene-mediated cell death and plant resistance against both incompatible pathogens Tobacco mosaic virus and Pseudomonas syringae pv tomato DC3000, as well as compatible pathogen P. syringae pv tabaci”
Claim · model-written summary
GAPC silencing enhanced resistance to Pseudomonas syringae pv tomato DC3000.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- silencing of GAPCse0
- Interactor
- Pseudomonas syringae pv tomato DC3000
Pseudomonas syringaee0pathogen - Outcome
- plant resistancee0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e0“silencing of GAPCs enhances N gene-mediated cell death and plant resistance against both incompatible pathogens Tobacco mosaic virus and Pseudomonas syringae pv tomato DC3000, as well as compatible pathogen P. syringae pv tabaci”
Claim · model-written summary
GAPC silencing enhanced resistance to P. syringae pv tabaci.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- silencing of GAPCse0
- Interactor
- P. syringae pv tabaciPseudomonas syringaee0pathogen
- Outcome
- plant resistancee0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e0“silencing of GAPCs enhances N gene-mediated cell death and plant resistance against both incompatible pathogens Tobacco mosaic virus and Pseudomonas syringae pv tomato DC3000, as well as compatible pathogen P. syringae pv tabaci”
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See all 43 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.