Abstract
Hydrogen peroxide (H2O2) was detected cytochemically in plant tissues during anoxia and re-oxygenation by transmission electron microscopy using its reaction with cerium chloride to produce electron dense precipitates of cerium perhydroxides. Anoxia-tolerant yellow flag iris (Iris pseudacorus) and rice (Oryza sativa), and anoxia-intolerant wheat (Triticum aestivum) and garden iris (Iris germanica) were used in the experiments. In all plants tested, anoxia and re-oxygenation increased H2O2 in plasma membranes and the apoplast. In the anoxia-tolerant species the response was delayed in time, and in highly tolerant I. pseudacorus plasma membrane associated H2O2 was detected only after 45 d of oxygen deprivation. Quantification of cerium precipitates showed a statistically significant increase in the amount of H2O2 caused by anoxia in wheat root meristematic tissue, but not in the anoxia-tolerant I. pseudacorus rhizome parenchyma. Formation of H2O2 under anoxia is considered mainly an enzymatic process (confirmed by an enzyme inhibition analysis) and is due to the trace amount of dissolved oxygen (below 10(-5) M) present in the experimental system. The data suggest oxidative stress is an integral part of oxygen deprivation stress, and emphasize the importance of the apoplast and plasma membrane in the development of the anoxic stress response.
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Claims 8
Claim · model-written summary
Anoxia and re-oxygenation increased H2O2 in plasma membranes and the apoplast in all plants tested.
- Taxon
- plantsViridiplantaee1
- Treatment
- anoxia and re-oxygenatione1
- Outcome
- H2O2 in plasma membranes and the apoplaste1
- Direction
- increasede1
Not reportedComparator
Verbatim
e1“In all plants tested, anoxia and re-oxygenation increased H2O2 in plasma membranes and the apoplast”
Claim · model-written summary
In highly tolerant Iris pseudacorus, plasma membrane associated H2O2 appeared only after 45 days of oxygen deprivation.
- Taxon
- I. pseudacorus
.jpg?width=60)
Iris pseudacoruse3 - Treatment
- oxygen deprivatione3
- Outcome
- plasma membrane associated H2O2e3
- Value
- 45 de3
- Qualifier
- only aftere3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e3“in highly tolerant I. pseudacorus plasma membrane associated H2O2 was detected only after 45 d of oxygen deprivation”
Claim · model-written summary
The H2O2 response to anoxia was delayed in time in the anoxia-tolerant species.
Verbatim
e2“In the anoxia-tolerant species the response was delayed in time”
Claim · model-written summary
Anoxia caused a statistically significant increase in H2O2 in wheat root meristematic tissue.
- Taxon
- wheat
Triticum aestivume4 - Organ / stage
- root meristematic tissuee4
- Treatment
- anoxiae4
- Outcome
- amount of H2O2e4
- Direction
- increasee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Quantification of cerium precipitates showed a statistically significant increase in the amount of H2O2 caused by anoxia in wheat root meristematic tissue”
Claim · model-written summary
Anoxia did not change H2O2 in the rhizome parenchyma of the anoxia-tolerant I. pseudacorus.
- Taxon
- I. pseudacorusIris pseudacoruse5
- Organ / stage
- rhizome parenchymae5
- Treatment
- anoxiae4
- Outcome
- amount of H2O2e4
- Direction
- no_changeinferrede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Quantification of cerium precipitates showed a statistically significant increase in the amount of H2O2 caused by anoxia in wheat root meristematic tissue”
e5“but not in the anoxia-tolerant I. pseudacorus rhizome parenchyma”
Claim · model-written summary
H2O2 formation under anoxia is considered mainly an enzymatic process.
- Taxon
- plantsViridiplantaee1
- Outcome
- Formation of H2O2 under anoxiae6
- Qualifier
- mainly an enzymatic processe6
Verbatim
e1“In all plants tested, anoxia and re-oxygenation increased H2O2 in plasma membranes and the apoplast”
e6“Formation of H2O2 under anoxia is considered mainly an enzymatic process (confirmed by an enzyme inhibition analysis)”
Claim · model-written summary
Oxidative stress is an integral part of oxygen deprivation stress.
- Taxon
- plantsViridiplantaee1
- Outcome
- oxidative stresse7
- Qualifier
- an integral part of oxygen deprivation stresse7
Verbatim
e1“In all plants tested, anoxia and re-oxygenation increased H2O2 in plasma membranes and the apoplast”
e7“The data suggest oxidative stress is an integral part of oxygen deprivation stress, and emphasize the importance of the apoplast and plasma membrane in the development of the anoxic stress response”
Claim · model-written summary
The apoplast and plasma membrane are important in the development of the anoxic stress response.
- Taxon
- plantsViridiplantaee1
- Outcome
- anoxic stress responsee7
- Qualifier
- the importance of the apoplast and plasma membranee7
Verbatim
e1“In all plants tested, anoxia and re-oxygenation increased H2O2 in plasma membranes and the apoplast”
e7“The data suggest oxidative stress is an integral part of oxygen deprivation stress, and emphasize the importance of the apoplast and plasma membrane in the development of the anoxic stress response”
The same questions in other papers
flooding · physiology: oxidative · Triticum aestivum (wheat)Results differ
19 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
18 papers more in this group
See all 22 papers →flooding · physiology: oxidative · Iris pseudacorusResults agree
1 paper in the same direction as this paper
- no effect
flooding · physiology: oxidative · Viridiplantae (plants)Results agree
2 papers in the same direction as this paper
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.