Abstract
Experiments were undertaken with embryo-less ovules of Pisum sativum to study the influence of apoplastic osmolality on seed coat import and seed coat unloading.11CO2 pulse labelling along with collimated monitoring of plant tissues were used with attached ovules to measure continuously and simultaneously total pod import, import into a modified ovule and photo-assimilate washout from the seed coat of the ovule into a flow-through bathingsolution. Our results indicated that seed coat import was immediately affected by a change in the applied bathing solution osmolality, with a decrease in osmolality lowering seed coat import and an increase in osmolality increasing import. 11C-photo-assimilate washout from attached ovules was found to respond in a similar manner to the apoplastic osmolality. However, the osmotic effect on 11C-washout was a delayed response and it appears that the majority of this observed response was due to the alteration in seed coat tracer import. Further experiments with 14C-labelled, excised seed coat halves (i.e. no further import) supported this hypothesis by demonstrating that seed coat unloading (measured as 14C-photo-assimilate washout) was actually enhanced at a low solution osmolality. PCMBS had no effect on seed coat import or washout in attached, modified ovules, suggesting that photo-assimilate unloading from seed coats of Pisum does not involve a carrier protein. Studies of the spatial distribution of imported 14C in Pisum seed coats further suggest that this unloading, into the apoplast, occurs from non-phloem cell types, and that the movement of photo-assimilates from the sieve elements to the terminal unloading site occurs via symplastic transport.
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Claims 9
Claim · model-written summary
decrease in osmolality changed Pisum seed coat import.
- Taxon
- Pisum sativum
Lathyrus oleraceuse0e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a decrease in osmolality lowering seed coat import and an increase in osmolality increasing import”
e1“embryo-less ovules of Pisum sativum”
Claim · model-written summary
increase in osmolality changed Pisum seed coat import.
- Taxon
- Pisum sativumLathyrus oleraceuse0e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a decrease in osmolality lowering seed coat import and an increase in osmolality increasing import”
e1“embryo-less ovules of Pisum sativum”
Claim · model-written summary
Attached ovule photo-assimilate washout responded similarly to apoplastic osmolality.
- Taxon
- Pisum sativumLathyrus oleraceuse0e1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“11C-photo-assimilate washout from attached ovules was found to respond in a similar manner to the apoplastic osmolality.”
e1“embryo-less ovules of Pisum sativum”
Claim · model-written summary
The washout response to osmolality was delayed and largely attributed to changed seed coat tracer import.
- Taxon
- Pisum sativumLathyrus oleraceuse0e1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the osmotic effect on 11C-washout was a delayed response and it appears that the majority of this observed response was due to the alteration in seed coat tracer import.”
e1“embryo-less ovules of Pisum sativum”
Claim · model-written summary
Low solution osmolality enhanced unloading from excised Pisum seed coats.
- Taxon
- Pisum sativumLathyrus oleraceuse0e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seed coat unloading (measured as 14C-photo-assimilate washout) was actually enhanced at a low solution osmolality.”
e1“embryo-less ovules of Pisum sativum”
Claim · model-written summary
PCMBS did not change seed coat import or washout in attached modified Pisum ovules.
LathyrusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PCMBS had no effect on seed coat import or washout in attached, modified ovules”
e1“seed coats of Pisum”
Claim · model-written summary
The authors inferred that Pisum seed coat unloading does not involve a carrier protein.
- Organ / stage
- seed coatse0
- Outcome
- photo-assimilate unloadinge0
- Qualifier
- does not involve a carrier proteine0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“photo-assimilate unloading from seed coats of Pisum does not involve a carrier protein.”
Claim · model-written summary
Pisum seed coat unloading into the apoplast was inferred to occur from non-phloem cells.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this unloading, into the apoplast, occurs from non-phloem cell types, and that the movement of photo-assimilates from the sieve elements to the terminal unloading site occurs via symplastic transport.”
e1“Pisum seed coats”
Claim · model-written summary
Photo-assimilates were inferred to move from sieve elements to the unloading site by symplastic transport.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this unloading, into the apoplast, occurs from non-phloem cell types, and that the movement of photo-assimilates from the sieve elements to the terminal unloading site occurs via symplastic transport.”
e1“Pisum seed coats”