Abstract
Using a new Arabidopsis (Arabidopsis thaliana) mutant (Atnrt2.1-nrt2.2) we confirm that concomitant disruption of NRT2.1 and NRT2.2 reduces inducible high-affinity transport system (IHATS) by up to 80%, whereas the constitutive high-affinity transport system (CHATS) was reduced by 30%. Nitrate influx via the low-affinity transport system (LATS) was unaffected. Shoot-to-root ratios were significantly reduced compared to wild-type plants, the major effect being upon shoot growth. In another mutant uniquely disrupted in NRT2.1 (Atnrt2.1), IHATS was reduced by up to 72%, whereas neither the CHATS nor the LATS fluxes were significantly reduced. Disruption of NRT2.1 in Atnrt2.1 caused a consistent and significant reduction of shoot-to-root ratios. IHATS influx and shoot-to-root ratios were restored to wild-type values when Atnrt2.1-nrt2.2 was transformed with a NRT2.1 cDNA isolated from Arabidopsis. Disruption of NRT2.2 in Atnrt2.2 reduced IHATS by 19% and this reduction was statistically significant only at 6 h after resupply of nitrate to nitrogen-deprived plants. Atnrt2.2 showed no significant reduction of CHATS, LATS, or shoot-to-root ratios. These results define NRT2.1 as the major contributor to IHATS. Nevertheless, when maintained on agar containing 0.25 mm KNO(3) as the sole nitrogen source, Atnrt2.1-nrt2.2 consistently exhibited greater stress and growth reduction than Atnrt2.1. Evidence from real-time PCR revealed that NRT2.2 transcript abundance was increased almost 3-fold in Atnrt2.1. These findings suggest that NRT2.2 normally makes only a small contribution to IHATS, but when NRT2.1 is lost, this contribution increases, resulting in a partial compensation.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 12
Claim · model-written summary
Double disruption reduced inducible high-affinity nitrate transport by up to 80%.
- Taxon
- Arabidopsis (Arabidopsis thaliana)
Arabidopsis thalianae0 - Material
- Atnrt2.1-nrt2.2e0
- Treatment
- concomitant disruption of NRT2.1 and NRT2.2e0
- Gene
- NRT2.1 and NRT2.2e0
- Outcome
- inducible high-affinity transport system (IHATS)e0
- Direction
- reducese0
- Value
- 80%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using a new Arabidopsis (Arabidopsis thaliana) mutant (Atnrt2.1-nrt2.2) we confirm that concomitant disruption of NRT2.1 and NRT2.2 reduces inducible high-affinity transport system (IHATS) by up to 80%”
Claim · model-written summary
Double disruption reduced constitutive high-affinity transport by 30%.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae1
- Treatment
- concomitant disruption of NRT2.1 and NRT2.2e1
- Outcome
- constitutive high-affinity transport system (CHATS)e0
- Direction
- reducede0
- Value
- 30%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“whereas the constitutive high-affinity transport system (CHATS) was reduced by 30%”
e1“Using a new Arabidopsis (Arabidopsis thaliana) mutant (Atnrt2.1-nrt2.2) we confirm that concomitant disruption of NRT2.1 and NRT2.2 reduces inducible high-affinity transport system (IHATS) by up to 80%”
Claim · model-written summary
Double disruption did not alter low-affinity nitrate influx.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae1
- Treatment
- concomitant disruption of NRT2.1 and NRT2.2e1
- Outcome
- Nitrate influx via the low-affinity transport system (LATS)e0
- Direction
- unaffectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nitrate influx via the low-affinity transport system (LATS) was unaffected.”
e1“Using a new Arabidopsis (Arabidopsis thaliana) mutant (Atnrt2.1-nrt2.2) we confirm that concomitant disruption of NRT2.1 and NRT2.2”
Claim · model-written summary
Double mutant shoot-to-root ratio was significantly lower than wild type, primarily through shoot growth.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae1
- Treatment
- Atnrt2.1-nrt2.2e1
- Outcome
- Shoot-to-root ratiose0
- Direction
- reducede0
- Qualifier
- major effect being upon shoot growthe0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot-to-root ratios were significantly reduced compared to wild-type plants, the major effect being upon shoot growth.”
e1“Using a new Arabidopsis (Arabidopsis thaliana) mutant (Atnrt2.1-nrt2.2)”
Claim · model-written summary
Disruption of NRT2.1 alone reduced inducible high-affinity transport by up to 72%.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae1
- Material
- Atnrt2.1e0
- Treatment
- mutant uniquely disrupted in NRT2.1e0
- Outcome
- IHATSe0
- Direction
- reducede0
- Value
- 72%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In another mutant uniquely disrupted in NRT2.1 (Atnrt2.1), IHATS was reduced by up to 72%”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
NRT2.1 disruption did not significantly reduce constitutive or low-affinity nitrate fluxes.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae2
- Treatment
- mutant uniquely disrupted in NRT2.1e1
- Outcome
- CHATS nor the LATS fluxese0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“neither the CHATS nor the LATS fluxes were significantly reduced”
e1“In another mutant uniquely disrupted in NRT2.1 (Atnrt2.1)”
e2“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
NRT2.1 disruption significantly reduced shoot-to-root ratios.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae1
- Treatment
- Disruption of NRT2.1e0
- Outcome
- shoot-to-root ratiose0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of NRT2.1 in Atnrt2.1 caused a consistent and significant reduction of shoot-to-root ratios.”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
NRT2.1 cDNA transformation restored IHATS influx and shoot-to-root ratios to wild-type values.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- transformed with a NRT2.1 cDNAe0
- Outcome
- IHATS influx and shoot-to-root ratiose0
- Direction
- restorede0
- Comparator
- wild-type valuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IHATS influx and shoot-to-root ratios were restored to wild-type values when Atnrt2.1-nrt2.2 was transformed with a NRT2.1 cDNA isolated from Arabidopsis.”
Claim · model-written summary
NRT2.2 disruption reduced IHATS by 19%, significant only six hours after nitrate resupply.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae1
- Treatment
- Disruption of NRT2.2e0
- Outcome
- IHATSe0
- Direction
- reducede0
- Value
- 19%e0
- Qualifier
- only at 6 h after resupply of nitrate to nitrogen-deprived plantse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of NRT2.2 in Atnrt2.2 reduced IHATS by 19% and this reduction was statistically significant only at 6 h after resupply of nitrate to nitrogen-deprived plants.”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
NRT2.2 disruption did not significantly reduce CHATS, LATS or shoot-to-root ratios.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae2
- Treatment
- Disruption of NRT2.2e1
- Outcome
- CHATS, LATS, or shoot-to-root ratiose0
- Direction
- no significant reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Atnrt2.2 showed no significant reduction of CHATS, LATS, or shoot-to-root ratios.”
e1“Disruption of NRT2.2 in Atnrt2.2”
e2“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
On low-nitrate agar, the double mutant showed greater stress and growth reduction than the NRT2.1 single mutant.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae1
- Treatment
- Atnrt2.1-nrt2.2e0
- Outcome
- stress and growth reductione0
- Direction
- greatere0
- Comparator
- Atnrt2.1e0
- Setting
- agar containing 0.25 mm KNO(3)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when maintained on agar containing 0.25 mm KNO(3) as the sole nitrogen source, Atnrt2.1-nrt2.2 consistently exhibited greater stress and growth reduction than Atnrt2.1.”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
NRT2.2 transcript abundance was almost threefold higher in the NRT2.1 mutant.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae1
- Material
- Atnrt2.1e0
- Gene
- NRT2.2e0
- Outcome
- NRT2.2 transcript abundancee0
- Direction
- increasede0
- Value
- almost 3-folde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NRT2.2 transcript abundance was increased almost 3-fold in Atnrt2.1.”
e1“Arabidopsis (Arabidopsis thaliana)”
The same questions in other papers
loss of function · nutrient: content · Arabidopsis thaliana (thale cress)Positive and negative results
18 papers in the same direction as this paper · 7 in the opposite direction
- negative
- no effect
- positive
22 papers more in this group
See all 26 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.