Abstract
1. Cotton cotyledon tissue was cultured in inorganic nutrient solutions containing various concentrations of 2,4-D. The nucleic acids and proteins of various cellular components were determined before and after a 48-hour culture period. The phosphatase activity, respiration, and growth rates were also determined. 2. Upon culture most of the RNA in the microsome fraction and about one-half that in the soluble protein was lost. There was an increase of RNA in the chloroplast fraction and little or no change of nucleic acid in the mitochondria and nuclei. There were similar trends for protein changes. 3. At concentrations of 10-4 and 10-3 M, 2,4-D prevented the changes in RNA and protein content. Similar concentrations of 2,4-D stimulated respiration, but the higher resulted in a decreased growth of the tissue as measured by fresh weight. 2,4-D was also found to prevent changes in phosphatase activity during culture of the tissue.
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Claims 9
Claim · model-written summary
Most of the RNA in the microsome fraction of cotton cotyledon tissue was lost upon culture.
- Taxon
- Cotton
Gossypium hirsutume0 - Organ / stage
- cotyledon tissuee0
- Outcome
- RNA in the microsome fractione1
- Direction
- loste1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotton cotyledon tissue was cultured in inorganic nutrient solutions containing various concentrations of 2,4-D”
e1“Upon culture most of the RNA in the microsome fraction and about one-half that in the soluble protein was lost”
Claim · model-written summary
About one-half of the RNA in the soluble protein of cotton cotyledon tissue was lost upon culture.
- Taxon
- CottonGossypium hirsutume0
- Organ / stage
- cotyledon tissuee0
- Outcome
- that in the soluble proteine1
- Direction
- loste1
- Value
- about one-halfe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotton cotyledon tissue was cultured in inorganic nutrient solutions containing various concentrations of 2,4-D”
e1“Upon culture most of the RNA in the microsome fraction and about one-half that in the soluble protein was lost”
Claim · model-written summary
RNA in the chloroplast fraction increased upon culture of cotton cotyledon tissue.
- Taxon
- CottonGossypium hirsutume0
- Organ / stage
- cotyledon tissuee0
- Outcome
- RNA in the chloroplast fractione1
- Direction
- increasee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotton cotyledon tissue was cultured in inorganic nutrient solutions containing various concentrations of 2,4-D”
e1“There was an increase of RNA in the chloroplast fraction”
Claim · model-written summary
Nucleic acid in the mitochondria and nuclei showed little or no change upon culture.
- Taxon
- CottonGossypium hirsutume0
- Organ / stage
- cotyledon tissuee0
- Outcome
- nucleic acid in the mitochondria and nucleie1
- Direction
- little or no changee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotton cotyledon tissue was cultured in inorganic nutrient solutions containing various concentrations of 2,4-D”
e1“little or no change of nucleic acid in the mitochondria and nuclei”
Claim · model-written summary
Protein changes during culture of cotton cotyledon tissue followed similar trends.
- Taxon
- CottonGossypium hirsutume0
- Organ / stage
- cotyledon tissuee0
- Outcome
- protein changese1
- Qualifier
- similar trendse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotton cotyledon tissue was cultured in inorganic nutrient solutions containing various concentrations of 2,4-D”
e1“There were similar trends for protein changes”
Claim · model-written summary
At 10-4 and 10-3 M, 2,4-D prevented changes in RNA and protein content of cotton cotyledon tissue during culture.
- Taxon
- CottonGossypium hirsutume0
- Treatment
- At concentrations of 10-4 and 10-3 M, 2,4-De1
- Outcome
- changes in RNA and protein contente1
- Direction
- preventede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotton cotyledon tissue was cultured in inorganic nutrient solutions containing various concentrations of 2,4-D”
e1“At concentrations of 10-4 and 10-3 M, 2,4-D prevented the changes in RNA and protein content”
Claim · model-written summary
Similar concentrations of 2,4-D stimulated respiration of cotton cotyledon tissue.
- Taxon
- CottonGossypium hirsutume0
- Treatment
- Similar concentrations of 2,4-De1
- Outcome
- respiratione1
- Direction
- stimulatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotton cotyledon tissue was cultured in inorganic nutrient solutions containing various concentrations of 2,4-D”
e1“Similar concentrations of 2,4-D stimulated respiration”
Claim · model-written summary
The higher 2,4-D concentration decreased growth of cotton cotyledon tissue, measured as fresh weight.
- Taxon
- CottonGossypium hirsutume0
- Treatment
- 2,4-De0
- Outcome
- growth of the tissue as measured by fresh weighte1
- Direction
- decreasede1
- Qualifier
- the highere1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotton cotyledon tissue was cultured in inorganic nutrient solutions containing various concentrations of 2,4-D”
e1“but the higher resulted in a decreased growth of the tissue as measured by fresh weight”
Claim · model-written summary
2,4-D prevented changes in phosphatase activity during culture of cotton cotyledon tissue.
- Taxon
- CottonGossypium hirsutume0
- Treatment
- 2,4-De1
- Outcome
- changes in phosphatase activitye1
- Direction
- prevente1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotton cotyledon tissue was cultured in inorganic nutrient solutions containing various concentrations of 2,4-D”
e1“2,4-D was also found to prevent changes in phosphatase activity during culture of the tissue”
The same questions in other papers
2,4-D · growth · Gossypium hirsutum (cotton)Positive and negative results
7 papers in the same direction as this paper · 12 in the opposite direction
- positive
- positive
- positive
16 papers more in this group
See all 20 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.