Abstract

Abstract Embryos of Scots pine ( Pinus sylvestris ), Corsican pine ( P. nigra var. maritima ) and Lodgepole pine ( P. contorta ) were infected in vitro with Heterobasidion annosum and the activities of 1,3‐β‐glucanase, chitinase and β‐glucosidase were monitored. Both the infected and control embryos of the three pine species showed the presence of the enzymes. Statistical analysis showed no significant increase (P > 0.05) in the levels of 1,3‐β‐glucanase in infected embryos for all the three pine species. However, there was a significant increase in levels of chitinase in infected embryos of P. contorta and P. nigra var. maritima and also in levels of β‐glucosidase in infected embryos of all three of the pine species. The study has therefore revealed that constitutive levels of glucanohydrolase enzymes exist in the embryos of the three pine species and that the induction of chitinase and β‐glucosidase was a factor in the response of the embryos to stimulation by H. annosum infection.

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Claims 4

Observationobservedoriginal resultmeasured

Claim · model-written summary

Constitutive levels of glucanohydrolase enzymes exist in the embryos of the three pine species.

Taxon
Scots, Corsican and lodgepole pinest0
Organ / stage
embryose1
Outcome
constitutive levels of glucanohydrolase enzymese1

Verbatim

t0

“Glucanohydrolase enzyme activity in embryos of Scots, Corsican and lodgepole pines infected in vitro with Heterobasidion annosum”

✓ located verbatim in the source · title, characters 0–128 · show in text ↑
e1

“constitutive levels of glucanohydrolase enzymes exist in the embryos of the three pine species”

✓ located verbatim in the source · abstract, characters 766–860 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Infection did not significantly increase 1,3‐β‐glucanase levels in pine embryos.

Taxon
Scots, Corsican and lodgepole pinest0
Treatment
infectede2
Interactor
Heterobasidion annosumHeterobasidion annosumt0pathogen
Outcome
levels of 1,3‐β‐glucanasee2
Direction
increasee2
Comparator
control embryose0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Heterobasidion annosum
Heterobasidion annosum · InteractorJames Lindsey · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Glucanohydrolase enzyme activity in embryos of Scots, Corsican and lodgepole pines infected in vitro with Heterobasidion annosum”

✓ located verbatim in the source · title, characters 0–128 · show in text ↑
e0

“Both the infected and control embryos of the three pine species showed the presence of the enzymes”

✓ located verbatim in the source · abstract, characters 263–361 · show in text ↑
e2

“Statistical analysis showed no significant increase (P > 0.05) in the levels of 1,3‐β‐glucanase in infected embryos for all the three pine species”

✓ located verbatim in the source · abstract, characters 363–509 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Chitinase levels increased significantly in infected embryos of P. contorta and P. nigra var. maritima.

Taxon
P. contorta and P. nigra var. maritimaPinus contortaPinus nigra subsp. laricioe3
Treatment
infectede3
Interactor
Heterobasidion annosumHeterobasidion annosumt0pathogen
Outcome
levels of chitinasee3
Direction
increasee3
Comparator
control embryose0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pinus contorta
Pinus contorta · TaxonWalter Siegmund · CC BY 2.5 · Wikimedia Commons ↗
Pinus nigra subsp. laricio
Pinus nigra subsp. laricio · Taxon · picture of the speciesKarl Gruber · CC BY-SA 3.0 · Wikimedia Commons ↗
Heterobasidion annosum
Heterobasidion annosum · InteractorJames Lindsey · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Glucanohydrolase enzyme activity in embryos of Scots, Corsican and lodgepole pines infected in vitro with Heterobasidion annosum”

✓ located verbatim in the source · title, characters 0–128 · show in text ↑
e0

“Both the infected and control embryos of the three pine species showed the presence of the enzymes”

✓ located verbatim in the source · abstract, characters 263–361 · show in text ↑
e3

“there was a significant increase in levels of chitinase in infected embryos of P. contorta and P. nigra var. maritima”

✓ located verbatim in the source · abstract, characters 520–637 · show in text ↑
Mechanismauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

Induction of chitinase and β-glucosidase was a factor in the embryos response to Heterobasidion annosum infection.

Taxon
Scots, Corsican and lodgepole pinest0
Interactor
H. annosumHeterobasidion annosume6pathogen
Gene
chitinasee7
Outcome
response of the embryose6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Heterobasidion annosum
Heterobasidion annosum · InteractorJames Lindsey · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Glucanohydrolase enzyme activity in embryos of Scots, Corsican and lodgepole pines infected in vitro with Heterobasidion annosum”

✓ located verbatim in the source · title, characters 0–128 · show in text ↑
e6

“was a factor in the response of the embryos to stimulation by H. annosum infection”

✓ located verbatim in the source · abstract, characters 915–997 · show in text ↑
e7

“the induction of chitinase”

✓ located verbatim in the source · abstract, characters 870–896 · show in text ↑