Abstract
The 2- to 4-fold rise in particle-bound beta-glucan synthetase (uridine diphosphate-glucose: beta-1, 4-glucan glucosyltransferase) activity that can be induced by indoleacetic acid in pea stem tissue is not prevented by concentrations of actinomycin D or cycloheximide that inhibit growth and macromolecule synthesis. The rise is concluded to be a hormonally induced activation of previously existing, reversibly deactivated enzyme. The activation is not a direct allosteric effect of indoleacetic acid or sugars. It is blocked by inhibitors of energy metabolism, by 2-deoxyglucose, and by high osmolarity, but not by Ca(2+) at concentrations that inhibit auxin-induced elongation and prevent promotion of sugar uptake by indoleacetic acid, and not by alpha, alpha'-dipyridyl at concentrations that inhibit formation of hydroxyproline. Regulation of the system could be due either to an ATP-dependent activating reaction affecting this enzyme, or to changes in levels of a primer or a lipid cofactor.
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Claims 10
Claim · model-written summary
Indoleacetic acid induced a 2- to 4-fold rise in pea stem beta-glucan synthetase activity.
- Taxon
- pea
Lathyrus oleraceuse0 - Organ / stage
- stem tissuee0
- Treatment
- indoleacetic acide0
- Gene
- beta-glucan synthetasee0
- Outcome
- particle-bound beta-glucan synthetasee0
- Direction
- risee0
- Value
- 2- to 4-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 2- to 4-fold rise in particle-bound beta-glucan synthetase (uridine diphosphate-glucose: beta-1, 4-glucan glucosyltransferase) activity that can be induced by indoleacetic acid in pea stem tissue”
Claim · model-written summary
Actinomycin D did not prevent the auxin-induced enzyme activity rise.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- actinomycin De0
- Outcome
- not preventede0
- Direction
- not preventede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“is not prevented by concentrations of actinomycin D or cycloheximide that inhibit growth and macromolecule synthesis”
t0“Pea”
Claim · model-written summary
Cycloheximide did not prevent the auxin-induced enzyme activity rise.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- cycloheximidee0
- Outcome
- not preventede0
- Direction
- not preventede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“is not prevented by concentrations of actinomycin D or cycloheximide that inhibit growth and macromolecule synthesis”
t0“Pea”
Claim · model-written summary
The activity rise was interpreted as activation of preexisting enzyme.
- Taxon
- peaLathyrus oleraceust0
- Outcome
- hormonally induced activatione0
- Qualifier
- previously existing, reversibly deactivated enzymee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rise is concluded to be a hormonally induced activation of previously existing, reversibly deactivated enzyme”
t0“Pea”
Claim · model-written summary
Activation was not a direct allosteric effect of indoleacetic acid or sugars.
- Taxon
- peaLathyrus oleraceust0
- Compound
- indoleacetic acid or sugarse0
- Outcome
- activatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activation is not a direct allosteric effect of indoleacetic acid or sugars”
t0“Pea”
Claim · model-written summary
Energy metabolism inhibitors blocked enzyme activation.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- inhibitors of energy metabolisme0
- Outcome
- blockede0
- Direction
- blockede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It is blocked by inhibitors of energy metabolism”
t0“Pea”
Claim · model-written summary
2-deoxyglucose blocked enzyme activation.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- 2-deoxyglucosee0
Not reportedComparatorOutcomeDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“by 2-deoxyglucose”
t0“Pea”
Claim · model-written summary
High osmolarity blocked enzyme activation.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- high osmolaritye0
Not reportedComparatorOutcomeDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“by high osmolarity”
t0“Pea”
Claim · model-written summary
Calcium did not block enzyme activation at doses that inhibit elongation.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- Ca(2+)e0
- Outcome
- auxin-induced elongatione0
- Direction
- inhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but not by Ca(2+) at concentrations that inhibit auxin-induced elongation”
t0“Pea”
Claim · model-written summary
Alpha, alpha-prime-dipyridyl did not block enzyme activation.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- alpha, alpha'-dipyridyle0
- Outcome
- formation of hydroxyprolinee0
- Direction
- inhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“and not by alpha, alpha'-dipyridyl at concentrations that inhibit formation of hydroxyproline”
t0“Pea”
The same questions in other papers
calcium(2+) · physiology: hormones · Lathyrus oleraceus (pea)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- negative
indole-3-acetic acid · molecular: metabolites · Lathyrus oleraceus (pea)Positive and negative results
11 papers in the same direction as this paper · 6 in the opposite direction · 1 reporting no difference from an alternative
- positive
- positive
- positive
15 papers more in this group
See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.