Abstract
Using aseptic plantlets obtained from stem node explants of hybrid red miniature rose (Rosa hybrida cv. Fairy Dance), the effects of shoot physiological status, medium ingredients, and culture thermoperiod on in vitro flowering were evaluated. Shoot height, subculture media for shoot multiplication, sucrose concentration, plant growth regulators (PGRs), mineral substances in media, and thermoperiod had a significant effect on the percentage of in vitro flowering. Shoots 3 ± 0.2 or 2 ± 0.2 cm in height cultured on Murashige and Skoog (MS) medium containing 2.0 mg dm-3 6-benzyladenine (BA), 0.2 mg dm-3 α-naphthaleneacetic acid (NAA), and 20 g dm-3 sucrose were more suitable for in vitro flowering than shoots 4 ± 0.2, or 5 ± 0.2 cm in height. The most suitable sucrose concentration for in vitro flowering was 50 g dm-3 and the most suitable PGRs were a combination of 3.0 mg dm-3 BA and 0.1 mg dm-3 NAA. Increasing the potassium nitrate to ammonium nitrate ratio or increasing the phosphate concentration in MS medium had a positive effect on in vitro flowering. The percentage of in vitro flowering was significantly higher at day/night temperature of 28/20 °C than at other constant temperatures. The percentage of in vitro flowering shoots reached 68.33 % despite the occurrence of abnormal flowers and some unique developmental patterns. It makes miniature rose a potentially new in vitro experimental platform for research on the molecular mechanisms of flowering ornamental plants.
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Claims 9
Claim · model-written summary
Shoot height and medium and temperature factors significantly affected flowering percentage.
- Taxon
- Rosa hybrida cv. Fairy Dance
Rosa hybrid cultivart0 - Outcome
- percentage of in vitro floweringe0
- Setting
- thermoperiode0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot height, subculture media for shoot multiplication, sucrose concentration, plant growth regulators (PGRs), mineral substances in media, and thermoperiod had a significant effect on the percentage of in vitro flowering”
t0“Rosa hybrida cv. Fairy Dance”
Claim · model-written summary
Shorter shoots under specified medium were more suitable than taller shoots.
- Taxon
- Rosa hybrida cv. Fairy DanceRosa hybrid cultivart0
- Treatment
- Shoots 3 ± 0.2 or 2 ± 0.2 cm in heighte0
- Outcome
- in vitro floweringe0
- Direction
- more suitablee0
- Comparator
- shoots 4 ± 0.2, or 5 ± 0.2 cm in heighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoots 3 ± 0.2 or 2 ± 0.2 cm in height cultured on Murashige and Skoog (MS) medium containing 2.0 mg dm-3 6-benzyladenine (BA), 0.2 mg dm-3 α-naphthaleneacetic acid (NAA), and 20 g dm-3 sucrose were more suitable for in vitro flowering than shoots 4 ± 0.2, or 5 ± 0.2 cm in height”
t0“Rosa hybrida cv. Fairy Dance”
Claim · model-written summary
The most suitable sucrose concentration was 50 g dm-3.
- Taxon
- Rosa hybrida cv. Fairy DanceRosa hybrid cultivart0
- Outcome
- in vitro floweringe0
- Direction
- most suitablee0
- Value
- 50 g dm-3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The most suitable sucrose concentration for in vitro flowering was 50 g dm-3”
t0“Rosa hybrida cv. Fairy Dance”
Claim · model-written summary
The most suitable regulator combination was BA and NAA at stated concentrations.
- Taxon
- Rosa hybrida cv. Fairy DanceRosa hybrid cultivart0
- Outcome
- most suitable PGRse0
- Value
- 3.0 mg dm-3 BA and 0.1 mg dm-3 NAAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the most suitable PGRs were a combination of 3.0 mg dm-3 BA and 0.1 mg dm-3 NAA”
t0“Rosa hybrida cv. Fairy Dance”
Claim · model-written summary
Increasing potassium nitrate to ammonium nitrate ratio improved flowering.
- Taxon
- Rosa hybrida cv. Fairy DanceRosa hybrid cultivart0
- Treatment
- Increasing the potassium nitrate to ammonium nitrate ratioe0
- Outcome
- in vitro floweringe0
- Direction
- positive effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increasing the potassium nitrate to ammonium nitrate ratio or increasing the phosphate concentration in MS medium had a positive effect on in vitro flowering”
t0“Rosa hybrida cv. Fairy Dance”
Claim · model-written summary
Increasing phosphate concentration improved flowering.
- Taxon
- Rosa hybrida cv. Fairy DanceRosa hybrid cultivart0
- Treatment
- increasing the phosphate concentration in MS mediume0
- Outcome
- in vitro floweringe0
- Direction
- positive effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increasing the potassium nitrate to ammonium nitrate ratio or increasing the phosphate concentration in MS medium had a positive effect on in vitro flowering”
t0“Rosa hybrida cv. Fairy Dance”
Claim · model-written summary
Flowering percentage was significantly higher under 28/20 °C day/night temperature.
- Taxon
- Rosa hybrida cv. Fairy DanceRosa hybrid cultivart0
- Treatment
- day/night temperature of 28/20 °Ce0
- Outcome
- percentage of in vitro floweringe0
- Direction
- highere0
- Comparator
- other constant temperaturese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The percentage of in vitro flowering was significantly higher at day/night temperature of 28/20 °C than at other constant temperatures”
t0“Rosa hybrida cv. Fairy Dance”
Claim · model-written summary
In vitro flowering reached 68.33% with abnormal flowers and unusual developmental patterns.
- Taxon
- Rosa hybrida cv. Fairy DanceRosa hybrid cultivart0
- Outcome
- percentage of in vitro flowering shootse0
- Value
- 68.33 %e0
- Qualifier
- abnormal flowers and some unique developmental patternse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The percentage of in vitro flowering shoots reached 68.33 % despite the occurrence of abnormal flowers and some unique developmental patterns”
t0“Rosa hybrida cv. Fairy Dance”
Claim · model-written summary
The authors propose miniature rose as an in vitro flowering research platform.
Not reportedComparatorValue
Verbatim
e0“It makes miniature rose a potentially new in vitro experimental platform for research on the molecular mechanisms of flowering ornamental plants”
The same questions in other papers
in vitro · phenology · Rosa hybrid cultivarResults agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.