Abstract
SummaryA successful regeneration system from in vitro-derived leaves of Rosa hybrida cv. Apollo, via somatic embryogenesis, has been established. The influence of different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and zeatin on the induction of embryogenic calli was evaluated. The highest percentage of embryogenic callus induction (48.3%) was obtained on full-strength MS medium containing 1.0 mg l–1 2,4-D, while at higher concentrations (4.0 or 5.0 mg l–1 2,4-D) no embryogenic calli were observed. The induction of embryogenic calli also occurred on MS media containing 0.5, 1.0, 2.0, or 4.0 mg l–1 naphthaleneacetic acid (NAA). The lowest concentration of NAA (0.5 mg l–1) showed the highest rate of production of embryogenic calli. For maturation, embryogenic calli were transferred onto MS media containing different concentrations of 6-benzyladenine (BA), thidiazuron (TDZ), or abscisic acid (ABA) alone, or in various combinations. The highest rate of embryo maturation (85.0%) was observed on MS medium supplemented with 1.0 mg l–1 ABA alone. Mature embryos showed the highest rate of germination on full-strength MS medium containing 2.0 mg l–1 BA alone. After rooting, well-developed plantlets were maintained under greenhouse conditions. The morphological characteristics and flower colours of somatic embryo-derived plants were the same as the donor parent.
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Claims 7
Claim · model-written summary
Embryogenic callus induction peaked at 48.3% with 1.0 mg l–1 2,4-D.
- Taxon
- Rosa hybrida
Rosa hybrid cultivart0 - Material
- Apollot0
- Treatment
- full-strength MS medium containing 1.0 mg l–1 2,4-De0
- Outcome
- embryogenic callus inductione0
- Direction
- higheste0
- Value
- 48.3%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest percentage of embryogenic callus induction (48.3%) was obtained on full-strength MS medium containing 1.0 mg l–1 2,4-D”
t0“Rosa hybridaL. cv. Apollo”
Claim · model-written summary
No embryogenic calli appeared at 4.0 or 5.0 mg l–1 2,4-D.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Material
- Apollot0
- Treatment
- 4.0 or 5.0 mg l–1 2,4-De0
- Outcome
- no embryogenic calli were observede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at higher concentrations (4.0 or 5.0 mg l–1 2,4-D) no embryogenic calli were observed.”
t0“Rosa hybridaL. cv. Apollo”
Claim · model-written summary
NAA concentrations of 0.5, 1.0, 2.0 and 4.0 mg l–1 induced embryogenic calli.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Material
- Apollot0
- Treatment
- 0.5, 1.0, 2.0, or 4.0 mg l–1 naphthaleneacetic acid (NAA)e0
- Outcome
- induction of embryogenic callie0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The induction of embryogenic calli also occurred on MS media containing 0.5, 1.0, 2.0, or 4.0 mg l–1 naphthaleneacetic acid (NAA).”
t0“Rosa hybridaL. cv. Apollo”
Claim · model-written summary
The lowest NAA dose produced the highest embryogenic callus rate.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Material
- Apollot0
- Treatment
- NAA (0.5 mg l–1)e0
- Outcome
- rate of production of embryogenic callie0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The lowest concentration of NAA (0.5 mg l–1) showed the highest rate of production of embryogenic calli.”
t0“Rosa hybridaL. cv. Apollo”
Claim · model-written summary
Embryo maturation peaked at 85.0% with 1.0 mg l–1 ABA alone.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Material
- Apollot0
- Treatment
- MS medium supplemented with 1.0 mg l–1 ABA alonee0
- Outcome
- rate of embryo maturatione0
- Direction
- higheste0
- Value
- 85.0%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest rate of embryo maturation (85.0%) was observed on MS medium supplemented with 1.0 mg l–1 ABA alone.”
t0“Rosa hybridaL. cv. Apollo”
Claim · model-written summary
Mature embryos germinated at the highest rate on full-strength MS medium with 2.0 mg l–1 BA alone.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Material
- Apollot0
- Treatment
- full-strength MS medium containing 2.0 mg l–1 BA alonee0
- Outcome
- rate of germinatione0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mature embryos showed the highest rate of germination on full-strength MS medium containing 2.0 mg l–1 BA alone.”
t0“Rosa hybridaL. cv. Apollo”
Claim · model-written summary
Somatic embryo-derived plants matched the donor parent in morphology and flower color.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Material
- Apollot0
- Outcome
- morphological characteristics and flower colourse0
- Direction
- samee0
- Comparator
- donor parente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The morphological characteristics and flower colours of somatic embryo-derived plants were the same as the donor parent.”
t0“Rosa hybridaL. cv. Apollo”
The same questions in other papers
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- positive
2,4-D · growth · Rosa hybrid cultivarResults agree
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- positive
- positive
- positive
N-benzyladenine · growth · Rosa hybrid cultivarResults agree
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- positive
- positive
- positive
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16 papers in the same direction as this paper · 2 in the opposite direction
- no effect
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See all 19 papers →in vitro · phenology · Rosa hybrid cultivarResults agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.