Abstract
Haploid ( 2x ) roses derived from modern tetraploid breeding lines would allow for crosses to diploid species at the diploid level. In addition, inheritance studies are easier at the diploid level, using diploids derived from tetraploids possessing economically important traits. Haploidization of 4x roses through anther culture has not been successful due to challenges in callus induction and shoot regeneration. This study investigates rose anther responses to recently reported methods that optimize in vitro adventitious shoot regeneration in rose leaves. Anthers of three cultivars (Akito, Grand Gala, and Orlando) were put in a two-step callus induction (CI) and shoot regeneration procedure with varying CI factors. Experiment one (E1) compared continuous light/dark and silver nitrate (0,30,60 mg·L -1 ) and experiment two (E2) used the optimal E1 treatment comparing two and four weeks on CI media. Twenty-five anthers per treatment per cultivar were used in E1 and n = 100 for E2. Although no adventitious shoots were generated, callus formed on anther tissue and frequency of formation was variable across treatments. Continuous light resulted in 100% lethality. Darkness and silver nitrate (30 or 60 μ m ) favored callus generation and significant differences for callus generation were found among cultivars. Darkness and 30 μ m silver nitrate were used in E2. Two and four weeks on initiation media were not significantly different for generation of anther-derived callus. Identification of factors which optimize callus formation on rose anthers is a positive step toward reliably generating rose haploids.
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Claims 7
Claim · model-written summary
No adventitious shoots were generated.
- Taxon
- Rosa hybrida L.
Rosa hybrid cultivart0 - Organ / stage
- anther tissuee0
- Outcome
- no adventitious shoots were generatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Although no adventitious shoots were generated, callus formed on anther tissue and frequency of formation was variable across treatments”
t0“Rosa hybrida L.”
Claim · model-written summary
Callus formed on anthers with frequencies varying by treatment.
- Taxon
- Rosa hybrida L.Rosa hybrid cultivart0
- Organ / stage
- anther tissuee0
- Treatment
- treatmentse0
- Outcome
- frequency of formatione0
- Direction
- variablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“callus formed on anther tissue and frequency of formation was variable across treatments”
t0“Rosa hybrida L.”
Claim · model-written summary
Continuous light caused complete lethality.
- Taxon
- Rosa hybrida L.Rosa hybrid cultivart0
- Treatment
- Continuous lighte0
- Outcome
- lethalitye0
- Value
- 100%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Continuous light resulted in 100% lethality”
t0“Rosa hybrida L.”
Claim · model-written summary
Darkness favored callus generation.
- Taxon
- Rosa hybrida L.Rosa hybrid cultivart0
- Treatment
- Darknesse0
- Outcome
- callus generatione0
- Direction
- favorede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Darkness and silver nitrate (30 or 60 μ m ) favored callus generation”
t0“Rosa hybrida L.”
Claim · model-written summary
Silver nitrate at 30 or 60 μ m favored callus generation.
- Taxon
- Rosa hybrida L.Rosa hybrid cultivart0
- Treatment
- silver nitrate (30 or 60 μ m )e0
- Outcome
- callus generatione0
- Direction
- favorede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Darkness and silver nitrate (30 or 60 μ m ) favored callus generation”
t0“Rosa hybrida L.”
Claim · model-written summary
Callus generation differed significantly among cultivars.
- Taxon
- Rosa hybrida L.Rosa hybrid cultivart0
- Material
- cultivarse0
- Outcome
- callus generatione0
- Direction
- differencese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“significant differences for callus generation were found among cultivars”
t0“Rosa hybrida L.”
Claim · model-written summary
Two and four weeks on initiation media did not differ significantly for callus generation.
- Taxon
- Rosa hybrida L.Rosa hybrid cultivart0
- Treatment
- Two and four weeks on initiation mediae0
- Outcome
- generation of anther-derived calluse0
- Direction
- not significantly differente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two and four weeks on initiation media were not significantly different for generation of anther-derived callus”
t0“Rosa hybrida L.”
The same questions in other papers
light · growth · Rosa hybrid cultivarPositive and negative results
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See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.