Abstract
Effect of explant, site of leaflet, induction period in the dark and combinations of plant growth regulators on direct adventitious bud induction and plant regeneration of Rosa hybrida Samantha was investigated. The results showed that after an induction period of 8 d on MS medium with 1.5 mg L^(-1) TDZ and 0.05 mg L^(-1) NAA in the dark and a subculture on MS medium with 0.5 mg L^(-1) BA and 0.01 mg L^(-1) NAA under light, the best plant regeneration was obtained and the regeneration frequencies of leaflets and petioles were 51.8 and 10% respectively. There was no significant difference in regeneration ability between leaflets at different sites of the compound leaves, longer time of induction in the dark or high concentration of auxin would cause callus formation, which was disadvantageous for shoot regeneration, and the regeneration frequency was significantly reduced. This regeneration system could be applied for genetic transformation of this cultivar in the future.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
The specified dark induction and light subculture gave the best plant regeneration.
- Taxon
- Rosa hybrida cv. Samantha
Rosa hybrid cultivart0 - Treatment
- an induction period of 8 d on MS medium with 1.5 mg L^(-1) TDZ and 0.05 mg L^(-1) NAA in the dark and a subculture on MS medium with 0.5 mg L^(-1) BA and 0.01 mg L^(-1) NAA under lighte0
- Outcome
- plant regeneratione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after an induction period of 8 d on MS medium with 1.5 mg L^(-1) TDZ and 0.05 mg L^(-1) NAA in the dark and a subculture on MS medium with 0.5 mg L^(-1) BA and 0.01 mg L^(-1) NAA under light, the best plant regeneration was obtained”
t0“Rosa hybrida cv. Samantha”
Claim · model-written summary
Leaflet regeneration frequency was 51.8%.
- Taxon
- Rosa hybrida cv. SamanthaRosa hybrid cultivart0
- Organ / stage
- leafletse0
- Outcome
- regeneration frequenciese0
- Value
- 51.8e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“regeneration frequencies of leaflets and petioles were 51.8 and 10% respectively”
t0“Rosa hybrida cv. Samantha”
Claim · model-written summary
Petiole regeneration frequency was 10%.
- Taxon
- Rosa hybrida cv. SamanthaRosa hybrid cultivart0
- Organ / stage
- petiolese0
- Outcome
- regeneration frequenciese0
- Value
- 10%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“regeneration frequencies of leaflets and petioles were 51.8 and 10% respectively”
t0“Rosa hybrida cv. Samantha”
Claim · model-written summary
Regeneration ability did not significantly differ among leaflet sites.
- Taxon
- Rosa hybrida cv. SamanthaRosa hybrid cultivart0
- Organ / stage
- leafletse0
- Outcome
- regeneration abilitye0
- Direction
- no significant differencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no significant difference in regeneration ability between leaflets at different sites of the compound leaves”
t0“Rosa hybrida cv. Samantha”
Claim · model-written summary
Longer dark induction caused callus formation and reduced shoot regeneration.
- Taxon
- Rosa hybrida cv. SamanthaRosa hybrid cultivart0
- Treatment
- longer time of induction in the darke0
- Outcome
- regeneration frequencye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“longer time of induction in the dark or high concentration of auxin would cause callus formation, which was disadvantageous for shoot regeneration, and the regeneration frequency was significantly reduced”
t0“Rosa hybrida cv. Samantha”
Claim · model-written summary
High auxin concentration caused callus formation and reduced shoot regeneration.
- Taxon
- Rosa hybrida cv. SamanthaRosa hybrid cultivart0
- Treatment
- high concentration of auxine0
- Outcome
- regeneration frequencye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high concentration of auxin would cause callus formation, which was disadvantageous for shoot regeneration, and the regeneration frequency was significantly reduced”
t0“Rosa hybrida cv. Samantha”
Claim · model-written summary
The regeneration system could be used for future genetic transformation of the cultivar.
- Taxon
- Rosa hybrida cv. SamanthaRosa hybrid cultivart0
- Outcome
- applied for genetic transformation of this cultivar in the futuree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This regeneration system could be applied for genetic transformation of this cultivar in the future”
t0“Rosa hybrida cv. Samantha”
The same questions in other papers
1-naphthaleneacetic acid · growth · Rosa hybrid cultivarResults agree
8 papers in the same direction as this paper
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →N-benzyladenine · growth · Rosa hybrid cultivarResults agree
19 papers in the same direction as this paper
- positive
- positive
- positive
16 papers more in this group
See all 20 papers →class · growth · Rosa hybrid cultivarPositive and negative results
1 paper in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →light · growth · Rosa hybrid cultivarPositive and negative results
10 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
9 papers more in this group
See all 13 papers →in vitro · growth · Rosa hybrid cultivarPositive and negative results
16 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
15 papers more in this group
See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.