Abstract
The rooting of shoots of micropropagated Rosa hybrida cv. Madame Delbard was conducted on MS medium with 30 kg m-3 sucrose or on hydroponic medium (containing less mineral salts), under higher photosynthetic photon flux density (PPFD) (100 in comparison with 45 µmol m-2 s-1) and flushed by ambient air [AC, 340 µmol(CO2) mol-1] or by CO2-enriched air (EC, 2 500 µmol mol-1) and lower relative humidity (80-90 % vs. 96-99 %). This cultivation led to plantlets with longer roots and adventitious root formation. Net photosynthetic rate and ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) activities, RuBPCO/phosphoenolpyruvate carboxylase activities ratio, and starch accumulation increased under these conditions. After 14 d, plantlets had functional stomata and could be acclimated on open benches without gradual decrease in relative humidity. The percentage of survival was higher when the rooting took place in EC than in AC. However, the advantage acquired during rooting phase by plantlets cultured in liquid medium was not maintained after 4 weeks of acclimatisation.
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Claims 8
Claim · model-written summary
The cultivation conditions produced longer roots and adventitious roots.
- Taxon
- Rosa hybrida
Rosa hybrid cultivare0 - Material
- Madame Delbarde0
- Treatment
- higher photosynthetic photon flux density (PPFD)e2
- Outcome
- longer roots and adventitious root formatione1
- Direction
- longere1
- Comparator
- 45 µmol m-2 s-1e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rosa hybrida cv. Madame Delbard”
e1“This cultivation led to plantlets with longer roots and adventitious root formation”
e2“under higher photosynthetic photon flux density (PPFD) (100 in comparison with 45 µmol m-2 s-1) and flushed by ambient air [AC, 340 µmol(CO2) mol-1] or by CO2-enriched air (EC, 2 500 µmol mol-1) and lower relative humidity (80-90 % vs. 96-99 %)”
Claim · model-written summary
Net photosynthetic rate increased under these cultivation conditions.
- Taxon
- Rosa hybridaRosa hybrid cultivare0
- Treatment
- higher photosynthetic photon flux density (PPFD)e2
- Outcome
- Net photosynthetic ratee1
- Direction
- increasede1
- Comparator
- 45 µmol m-2 s-1e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rosa hybrida cv. Madame Delbard”
e1“Net photosynthetic rate and ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) activities, RuBPCO/phosphoenolpyruvate carboxylase activities ratio, and starch accumulation increased under these conditions”
e2“under higher photosynthetic photon flux density (PPFD) (100 in comparison with 45 µmol m-2 s-1)”
Claim · model-written summary
RuBPCO activity increased under these cultivation conditions.
- Taxon
- Rosa hybridaRosa hybrid cultivare0
- Treatment
- higher photosynthetic photon flux density (PPFD)e2
- Gene
- ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO)e1
- Outcome
- RuBPCO) activitiese1
- Direction
- increasede1
- Comparator
- 45 µmol m-2 s-1e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rosa hybrida cv. Madame Delbard”
e1“Net photosynthetic rate and ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) activities, RuBPCO/phosphoenolpyruvate carboxylase activities ratio, and starch accumulation increased under these conditions”
e2“under higher photosynthetic photon flux density (PPFD) (100 in comparison with 45 µmol m-2 s-1)”
Claim · model-written summary
The RuBPCO to phosphoenolpyruvate carboxylase activity ratio increased.
- Taxon
- Rosa hybridaRosa hybrid cultivare0
- Outcome
- RuBPCO/phosphoenolpyruvate carboxylase activities ratioe1
- Direction
- increasede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rosa hybrida cv. Madame Delbard”
e1“RuBPCO/phosphoenolpyruvate carboxylase activities ratio, and starch accumulation increased under these conditions”
Claim · model-written summary
Starch accumulation increased under these conditions.
- Taxon
- Rosa hybridaRosa hybrid cultivare0
- Outcome
- starch accumulatione1
- Direction
- increasede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rosa hybrida cv. Madame Delbard”
e1“starch accumulation increased under these conditions”
Claim · model-written summary
After 14 days, plantlets had functional stomata and could be acclimated on open benches without gradually lowering humidity.
- Taxon
- Rosa hybridaRosa hybrid cultivare0
- Organ / stage
- plantletse1
- Outcome
- functional stomata and could be acclimated on open benchese1
- Qualifier
- without gradual decrease in relative humiditye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rosa hybrida cv. Madame Delbard”
e1“After 14 d, plantlets had functional stomata and could be acclimated on open benches without gradual decrease in relative humidity”
Claim · model-written summary
Survival was higher after rooting in CO2-enriched air than in ambient air.
- Taxon
- Rosa hybridaRosa hybrid cultivare0
- Treatment
- CO2-enriched air (EC, 2 500 µmol mol-1)e2
- Outcome
- percentage of survivale1
- Direction
- highere1
- Comparator
- ambient air [AC, 340 µmol(CO2) mol-1]e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rosa hybrida cv. Madame Delbard”
e1“The percentage of survival was higher when the rooting took place in EC than in AC”
e2“ambient air [AC, 340 µmol(CO2) mol-1] or by CO2-enriched air (EC, 2 500 µmol mol-1)”
Claim · model-written summary
The rooting advantage of liquid medium was not maintained after four weeks of acclimatization.
- Taxon
- Rosa hybridaRosa hybrid cultivare0
- Treatment
- liquid mediume1
- Outcome
- advantage acquired during rooting phasee1
- Direction
- not maintainede1
- Qualifier
- after 4 weeks of acclimatisatione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rosa hybrida cv. Madame Delbard”
e1“the advantage acquired during rooting phase by plantlets cultured in liquid medium was not maintained after 4 weeks of acclimatisation”
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See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.