Abstract
In vitro propagation techniques were studied to establish a protocol for rapid and mass production of Rosa spp. cv. Eiffel Tower, an important and endangered cultivar. Several experiments were conducted through a two-stage procedure: multiplication stage and rooting of microshoots stage. For multiplication, the best results were obtained when nodal segments were cultured on MS medium supplemented with BAP at 1mg/l and/or Kinetin at 0.5 mg/l. The highest shoots number (3.17 shoots/explant) was obtained when 0.5 mg/l BAP was added to the medium. Kinetin at 0.5 mg/l produced the highest shoot number/explant (3.67 shoots), and shoot length (3.67 cm). The interaction between BAP at 1 mg/l plus kinetin at 0.5 mg/l gave the highest shoot number/explant (4 shoots). For rooting, the best interaction effect on rooting percentage (61.00%), highest number of roots/shoot (5.12) and root length (3.33 cm) were observed when the shoots were cultured on hormone-free MS medium and pH level was adjusted to 5.5. Moreover, adjusting the medium pH to 5.5 formed roots faster in 17.67 days followed by medium with pH level 4.5 (18 days). The medium with pH at 5.5 supplemented with 50 g/l sucrose produced the highest significant value of rooting (85%) and enhanced root formation in 7.5 days. The rooted plantlets were transplanted into the potting substrate and successfully acclimatized in the laboratory then transferred to the field with a survival rate of 55%. After 6 month, the plantlets reached ~ 22 cm in length and 5.50 leaves/plant
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 13
Claim · model-written summary
BAP at 0.5 mg/l yielded 3.17 shoots per explant.
- Taxon
- Rosa hybrida
Rosa hybrid cultivart0 - Material
- Eiffel Towert0
- Treatment
- 0.5 mg/l BAPe0
- Outcome
- shoots numbere0
- Direction
- higheste0
- Value
- 3.17 shoots/explante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest shoots number (3.17 shoots/explant) was obtained when 0.5 mg/l BAP was added to the medium.”
t0“Rosa hybrida cv. Eiffel Tower”
Claim · model-written summary
Kinetin at 0.5 mg/l produced 3.67 shoots per explant.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Treatment
- Kinetin at 0.5 mg/le0
- Outcome
- shoot number/explante0
- Direction
- higheste0
- Value
- 3.67 shootse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Kinetin at 0.5 mg/l produced the highest shoot number/explant (3.67 shoots), and shoot length (3.67 cm).”
t0“Rosa hybrida cv. Eiffel Tower”
Claim · model-written summary
Kinetin at 0.5 mg/l produced 3.67 cm shoot length.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Treatment
- Kinetin at 0.5 mg/le0
- Outcome
- shoot lengthe0
- Direction
- higheste0
- Value
- 3.67 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Kinetin at 0.5 mg/l produced the highest shoot number/explant (3.67 shoots), and shoot length (3.67 cm).”
t0“Rosa hybrida cv. Eiffel Tower”
Claim · model-written summary
Combined BAP and kinetin yielded four shoots per explant.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Treatment
- BAP at 1 mg/l plus kinetin at 0.5 mg/le0
- Outcome
- shoot number/explante0
- Direction
- higheste0
- Value
- 4 shootse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The interaction between BAP at 1 mg/l plus kinetin at 0.5 mg/l gave the highest shoot number/explant (4 shoots).”
t0“Rosa hybrida cv. Eiffel Tower”
Claim · model-written summary
Hormone-free MS medium at pH 5.5 yielded 61.00% rooting.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Treatment
- hormone-free MS medium and pH level was adjusted to 5.5e0
- Outcome
- rooting percentagee0
- Direction
- beste0
- Value
- 61.00%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the best interaction effect on rooting percentage (61.00%), highest number of roots/shoot (5.12) and root length (3.33 cm) were observed when the shoots were cultured on hormone-free MS medium and pH level was adjusted to 5.5.”
t0“Rosa hybrida cv. Eiffel Tower”
Claim · model-written summary
Hormone-free MS medium at pH 5.5 yielded 5.12 roots per shoot.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Treatment
- hormone-free MS medium and pH level was adjusted to 5.5e0
- Outcome
- number of roots/shoote0
- Direction
- higheste0
- Value
- 5.12e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“highest number of roots/shoot (5.12) and root length (3.33 cm) were observed when the shoots were cultured on hormone-free MS medium and pH level was adjusted to 5.5.”
t0“Rosa hybrida cv. Eiffel Tower”
Claim · model-written summary
Hormone-free MS medium at pH 5.5 yielded 3.33 cm roots.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Treatment
- hormone-free MS medium and pH level was adjusted to 5.5e0
- Outcome
- root lengthe0
- Direction
- higheste0
- Value
- 3.33 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“highest number of roots/shoot (5.12) and root length (3.33 cm) were observed when the shoots were cultured on hormone-free MS medium and pH level was adjusted to 5.5.”
t0“Rosa hybrida cv. Eiffel Tower”
Claim · model-written summary
Medium pH 5.5 formed roots in 17.67 days, faster than pH 4.5 at 18 days.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Treatment
- medium pH to 5.5e0
- Outcome
- formed rootse0
- Direction
- fastere0
- Value
- 17.67 dayse0
- Qualifier
- 18 dayse0
- Comparator
- medium with pH level 4.5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“adjusting the medium pH to 5.5 formed roots faster in 17.67 days followed by medium with pH level 4.5 (18 days).”
t0“Rosa hybrida cv. Eiffel Tower”
Claim · model-written summary
MS medium at pH 5.5 with sucrose yielded 85% rooting.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Treatment
- medium with pH at 5.5 supplemented with 50 g/l sucrosee0
- Outcome
- rootinge0
- Direction
- higheste0
- Value
- 85%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The medium with pH at 5.5 supplemented with 50 g/l sucrose produced the highest significant value of rooting (85%) and enhanced root formation in 7.5 days.”
t0“Rosa hybrida cv. Eiffel Tower”
Claim · model-written summary
MS medium at pH 5.5 with sucrose enhanced root formation in 7.5 days.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Treatment
- medium with pH at 5.5 supplemented with 50 g/l sucrosee0
- Outcome
- root formatione0
- Direction
- enhancede0
- Value
- 7.5 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The medium with pH at 5.5 supplemented with 50 g/l sucrose produced the highest significant value of rooting (85%) and enhanced root formation in 7.5 days.”
t0“Rosa hybrida cv. Eiffel Tower”
Claim · model-written summary
Transplanted rooted plantlets acclimatized and survived at 55%.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Outcome
- survival ratee0
- Value
- 55%e0
- Setting
- fielde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rooted plantlets were transplanted into the potting substrate and successfully acclimatized in the laboratory then transferred to the field with a survival rate of 55%.”
t0“Rosa hybrida cv. Eiffel Tower”
Claim · model-written summary
After six months plantlets reached approximately 22 cm.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Outcome
- lengthe0
- Value
- ~ 22 cme0
- Qualifier
- After 6 monthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After 6 month, the plantlets reached ~ 22 cm in length and 5.50 leaves/plant”
t0“Rosa hybrida cv. Eiffel Tower”
Claim · model-written summary
After six months plantlets had 5.50 leaves per plant.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Outcome
- leaves/plante0
- Value
- 5.50 leaves/plante0
- Qualifier
- After 6 monthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After 6 month, the plantlets reached ~ 22 cm in length and 5.50 leaves/plant”
t0“Rosa hybrida cv. Eiffel Tower”
The same questions in other papers
kinetin · growth · Rosa hybrid cultivarResults agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →sucrose · growth · Rosa hybrid cultivarResults agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →N-benzyladenine · growth · Rosa hybrid cultivarResults agree
19 papers in the same direction as this paper
- positive
- positive
- positive
16 papers more in this group
See all 20 papers →class · growth · Rosa hybrid cultivarPositive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- negative
- no effect
- positive
1 paper more in this group
See all 5 papers →in vitro · growth · Rosa hybrid cultivarPositive and negative results
16 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
15 papers more in this group
See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.