Paper · 2015 · Indian Journal of Horticulture

Callus induction and proliferation fromRosa hybridaleaf explants

Mohan Ram; K.V. Prasad; T. Janakiram; et al.

5
claims
10
verbatim quotes
3
citations
2
research areas
✓
Open access

Abstract

To establish a resourceful tissue culture system to induce anthocyanin pigments, research was conducted to obtain high quality callus from leaf explant of Rosa hybrida cv. Pusa Ajay. Of the different treatments employed for callus induction using Murashige and Skoog (MS) medium, a treatment combination of 19.60 μM indole3-butyric acid (IBA) + 4.65 μM kinetin (Kin) + 108.6 mM adenine sulphate (AdS) gave the maximum induction coefficient (97.75 ± 1.11%) along with maximum gain in callus biomass accumulation. This treatment also resulted in the lowest fresh-and dry-cell weight ratio (26.52) suggesting actual gain in callus biomass accumulation. Correlation analysis indicated a significant positive correlation between callus induction coefficient and cell fresh-(r = 0.939) and dry-(r = 0.951) weight.

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Claims 5

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The treatment gave the maximum callus induction coefficient of 97.75 ± 1.11%.

Taxon
Rosa hybridaRosa hybrid cultivare0
Material
Pusa Ajaye0
Organ / stage
leafe0
Treatment
19.60 μM indole3-butyric acid (IBA) + 4.65 μM kinetin (Kin) + 108.6 mM adenine sulphate (AdS)e1
Outcome
induction coefficiente1
Direction
maximume1
Value
97.75 ± 1.11%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Rosa hybrid cultivar
Rosa hybrid cultivar · Taxon · picture of its genus (no picture of exactly this name)Primazega · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“leaf explant of Rosa hybrida cv. Pusa Ajay.”

✓ located verbatim in the source · abstract, characters 139–182 · show in text ↑
e1

“19.60 μM indole3-butyric acid (IBA) + 4.65 μM kinetin (Kin) + 108.6 mM adenine sulphate (AdS) gave the maximum induction coefficient (97.75 ± 1.11%)”

✓ located verbatim in the source · abstract, characters 307–455 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The same treatment maximized callus biomass accumulation.

Taxon
Rosa hybridaRosa hybrid cultivare0
Treatment
19.60 μM indole3-butyric acid (IBA) + 4.65 μM kinetin (Kin) + 108.6 mM adenine sulphate (AdS)e1
Outcome
gain in callus biomass accumulatione1
Direction
maximume1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Rosa hybrid cultivar
Rosa hybrid cultivar · Taxon · picture of its genus (no picture of exactly this name)Primazega · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“leaf explant of Rosa hybrida cv. Pusa Ajay.”

✓ located verbatim in the source · abstract, characters 139–182 · show in text ↑
e1

“19.60 μM indole3-butyric acid (IBA) + 4.65 μM kinetin (Kin) + 108.6 mM adenine sulphate (AdS) gave the maximum induction coefficient (97.75 ± 1.11%) along with maximum gain in callus biomass accumulation.”

✓ located verbatim in the source · abstract, characters 307–511 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The same treatment produced the lowest fresh-to-dry cell weight ratio, 26.52.

Taxon
Rosa hybridaRosa hybrid cultivare0
Treatment
This treatmente1
Outcome
fresh-and dry-cell weight ratioe1
Direction
loweste1
Value
26.52e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Rosa hybrid cultivar
Rosa hybrid cultivar · Taxon · picture of its genus (no picture of exactly this name)Primazega · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“leaf explant of Rosa hybrida cv. Pusa Ajay.”

✓ located verbatim in the source · abstract, characters 139–182 · show in text ↑
e1

“This treatment also resulted in the lowest fresh-and dry-cell weight ratio (26.52)”

✓ located verbatim in the source · abstract, characters 512–594 · show in text ↑
Associationobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Callus induction coefficient correlated positively with cell fresh weight (r = 0.939).

Taxon
Rosa hybridaRosa hybrid cultivare0
Outcome
cell fresh-e1
Direction
positivee1
Value
r = 0.939e1
Comparator
callus induction coefficiente1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Rosa hybrid cultivar
Rosa hybrid cultivar · Taxon · picture of its genus (no picture of exactly this name)Primazega · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“leaf explant of Rosa hybrida cv. Pusa Ajay.”

✓ located verbatim in the source · abstract, characters 139–182 · show in text ↑
e1

“significant positive correlation between callus induction coefficient and cell fresh-(r = 0.939) and dry-(r = 0.951) weight.”

✓ located verbatim in the source · abstract, characters 683–807 · show in text ↑
Associationobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Callus induction coefficient correlated positively with cell dry weight (r = 0.951).

Taxon
Rosa hybridaRosa hybrid cultivare0
Outcome
dry-e1
Direction
positivee1
Value
r = 0.951e1
Comparator
callus induction coefficiente1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Rosa hybrid cultivar
Rosa hybrid cultivar · Taxon · picture of its genus (no picture of exactly this name)Primazega · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“leaf explant of Rosa hybrida cv. Pusa Ajay.”

✓ located verbatim in the source · abstract, characters 139–182 · show in text ↑
e1

“significant positive correlation between callus induction coefficient and cell fresh-(r = 0.939) and dry-(r = 0.951) weight.”

✓ located verbatim in the source · abstract, characters 683–807 · show in text ↑

adenine · growth · Rosa hybrid cultivarResults agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

kinetin · growth · Rosa hybrid cultivarResults agree

5 papers: 5 positive, 0 negative or no effect

4 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

indole-3-butyric acid · growth · Rosa hybrid cultivarResults agree

15 papers: 15 positive, 0 negative or no effect

14 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

11 papers more in this group

See all 15 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.