Abstract
Friable callus from leaf disks of Rosa hybrida `Tiffany' was initiated within two weeks under dark conditions and 25°C on Murashige and Skoog (MS) medium supplemented with 4 mg.liter -1 2,4-D. Callus was then transferred into MS medium containing 3 mg.liter -1 2,4-D. Within four weeks, rhizogenesis occurred on the callus surface. The rhizogenic calllus was subculture on MS medium plus 3 mg.liter -1 2,4-D every 4-6 weeks. Within six months from initial culture, somatic embryos were developed on the aging callus in darkness. Transfer of the aging callus with somatic embryos into 1/2 MS medium containing 1 mg.liter -1 kinetin and maintaining it under 46 μE m -2 s -1 light for 16 hrs. resulted in greening of the somatic embryos.
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Claims 4
Claim · model-written summary
Leaf disks formed friable callus within two weeks on 4 mg.liter -1 2,4-D in darkness at 25°C.
- Taxon
- Rosa hybrida
Rosa hybrid cultivare0 - Material
- `Tiffany'e0
- Organ / stage
- leaf diskse0
- Treatment
- 4 mg.liter -1 2,4-De0
- Outcome
- Friable calluse0
- Value
- within two weekse0
- Qualifier
- dark conditions and 25°Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Friable callus from leaf disks of Rosa hybrida `Tiffany' was initiated within two weeks under dark conditions and 25°C on Murashige and Skoog (MS) medium supplemented with 4 mg.liter -1 2,4-D”
Claim · model-written summary
Rhizogenesis occurred on callus within four weeks in medium containing 3 mg.liter -1 2,4-D.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Treatment
- 3 mg.liter -1 2,4-De0
- Outcome
- rhizogenesis occurred on the callus surfacee0
- Value
- Within four weekse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus was then transferred into MS medium containing 3 mg.liter -1 2,4-D. Within four weeks, rhizogenesis occurred on the callus surface”
t0“leaf disks of Rosa hybrida `Tiffany'”
Claim · model-written summary
Somatic embryos developed on aging callus in darkness within six months of initial culture.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Organ / stage
- aging calluse0
- Outcome
- somatic embryos were developede0
- Value
- Within six monthse0
- Qualifier
- in darknesse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Within six months from initial culture, somatic embryos were developed on the aging callus in darkness”
t0“leaf disks of Rosa hybrida `Tiffany'”
Claim · model-written summary
Transferring embryo-bearing callus to kinetin medium under light produced embryo greening.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Treatment
- 1/2 MS medium containing 1 mg.liter -1 kinetine0
- Outcome
- greening of the somatic embryose0
- Qualifier
- 46 μE m -2 s -1 light for 16 hrs.e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transfer of the aging callus with somatic embryos into 1/2 MS medium containing 1 mg.liter -1 kinetin and maintaining it under 46 μE m -2 s -1 light for 16 hrs. resulted in greening of the somatic embryos”
t0“leaf disks of Rosa hybrida `Tiffany'”
The same questions in other papers
kinetin · growth · Rosa hybrid cultivarResults agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →in vitro · growth · Rosa hybrid cultivarPositive and negative results
16 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
15 papers more in this group
See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.