Abstract
Interactive effect of plant growth regulators 6-Benzylaminopurine (BAP) (0, 2, 4 and 8 µM) and 1-Naphtalene acetic acid (NAA) (0, 0.05, 0.25 and 0.5 µM) in Van der Salm (VS) medium was used to optimize in vitro propagation of Rosa hybrida cv. Iceberg. Shoot proliferation and number of new leaves were measured as growth indicators. As the concentration of BAP was raised, growth rate increased with all of the above NAA concentrations. However, the highest number of axillary shoots and new leaves were produced with 4 µM BAP, which was considered the optimal level. A multiplication rate of 10 folds with a maximum number of axillary shoots (10.1) and new leaves per explant (25) were obtained in the medium containing 4 µM BAP plus 0.5 µM NAA. In vitro-derived shoots were used to investigate root initiation and growth by lowering the concentration of VS mineral salts and vitamins. Three strengths of VS (full, 1/2 and 1/4) were compared in semi-solid and liquid medium. The average number of roots (4.35) and root length (0.82 cm) were significantly higher in 1/4 strength VS. The highest percentage of rooting (93.33%) and number of roots (4.45) were significantly higher in semi-solid than liquid medium. The regenerated plantlets were successfully transferred to soil and the survival rates of the rooted plantlets transferred to soil were 70% and 90% in plants treated with semi-sold and liquid media, respectively.
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Claims 12
Claim · model-written summary
Increasing BAP concentration raised growth rate at every tested NAA concentration.
- Taxon
- Rosa hybrida
Rosa hybrid cultivart0 - Material
- Icebergt0
- Treatment
- BAPe0
- Outcome
- growth ratee0
- Direction
- increasede0
- Qualifier
- all of the above NAA concentrationse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“As the concentration of BAP was raised, growth rate increased with all of the above NAA concentrations”
t0“Rosa hybrida cv. Iceberg”
Claim · model-written summary
The highest axillary shoot count occurred with 4 µM BAP.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Material
- Icebergt0
- Treatment
- 4 µM BAPe0
- Outcome
- number of axillary shootse0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest number of axillary shoots and new leaves were produced with 4 µM BAP”
t0“Rosa hybrida cv. Iceberg”
Claim · model-written summary
The highest new leaf count occurred with 4 µM BAP.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Material
- Icebergt0
- Treatment
- 4 µM BAPe0
- Outcome
- new leavese0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest number of axillary shoots and new leaves were produced with 4 µM BAP”
t0“Rosa hybrida cv. Iceberg”
Claim · model-written summary
Medium containing 4 µM BAP and 0.5 µM NAA yielded tenfold multiplication.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Material
- Icebergt0
- Treatment
- 4 µM BAP plus 0.5 µM NAAe0
- Outcome
- multiplication ratee0
- Value
- 10 foldse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A multiplication rate of 10 folds with a maximum number of axillary shoots (10.1) and new leaves per explant (25) were obtained in the medium containing 4 µM BAP plus 0.5 µM NAA”
t0“Rosa hybrida cv. Iceberg”
Claim · model-written summary
The same medium produced 10.1 axillary shoots per explant.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Material
- Icebergt0
- Treatment
- 4 µM BAP plus 0.5 µM NAAe0
- Outcome
- number of axillary shootse0
- Direction
- maximume0
- Value
- 10.1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A multiplication rate of 10 folds with a maximum number of axillary shoots (10.1) and new leaves per explant (25) were obtained in the medium containing 4 µM BAP plus 0.5 µM NAA”
t0“Rosa hybrida cv. Iceberg”
Claim · model-written summary
The same medium produced 25 new leaves per explant.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Material
- Icebergt0
- Treatment
- 4 µM BAP plus 0.5 µM NAAe0
- Outcome
- new leaves per explante0
- Value
- 25e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“new leaves per explant (25) were obtained in the medium containing 4 µM BAP plus 0.5 µM NAA”
t0“Rosa hybrida cv. Iceberg”
Claim · model-written summary
Quarter-strength VS produced a significantly higher average root number of 4.35.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Material
- Icebergt0
- Treatment
- 1/4 strength VSe0
- Outcome
- average number of rootse0
- Direction
- highere0
- Value
- 4.35e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The average number of roots (4.35) and root length (0.82 cm) were significantly higher in 1/4 strength VS”
t0“Rosa hybrida cv. Iceberg”
Claim · model-written summary
Quarter-strength VS produced significantly higher root length of 0.82 cm.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Material
- Icebergt0
- Treatment
- 1/4 strength VSe0
- Outcome
- root lengthe0
- Direction
- highere0
- Value
- 0.82 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The average number of roots (4.35) and root length (0.82 cm) were significantly higher in 1/4 strength VS”
t0“Rosa hybrida cv. Iceberg”
Claim · model-written summary
Semi-solid medium gave significantly higher rooting percentage of 93.33% than liquid medium.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Material
- Icebergt0
- Treatment
- semi-solide0
- Outcome
- percentage of rootinge0
- Direction
- highere0
- Value
- 93.33%e0
- Comparator
- liquid mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest percentage of rooting (93.33%) and number of roots (4.45) were significantly higher in semi-solid than liquid medium”
t0“Rosa hybrida cv. Iceberg”
Claim · model-written summary
Semi-solid medium gave significantly higher root number of 4.45 than liquid medium.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Material
- Icebergt0
- Treatment
- semi-solide0
- Outcome
- number of rootse0
- Direction
- highere0
- Value
- 4.45e0
- Comparator
- liquid mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest percentage of rooting (93.33%) and number of roots (4.45) were significantly higher in semi-solid than liquid medium”
t0“Rosa hybrida cv. Iceberg”
Claim · model-written summary
Plantlets from semi-solid media survived soil transfer at 70%.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Material
- Icebergt0
- Treatment
- semi-solde0
- Outcome
- survival ratese0
- Value
- 70%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“survival rates of the rooted plantlets transferred to soil were 70% and 90% in plants treated with semi-sold and liquid media, respectively”
t0“Rosa hybrida cv. Iceberg”
Claim · model-written summary
Plantlets from liquid media survived soil transfer at 90%.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Material
- Icebergt0
- Treatment
- liquid mediae0
- Outcome
- survival ratese0
- Value
- 90%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“survival rates of the rooted plantlets transferred to soil were 70% and 90% in plants treated with semi-sold and liquid media, respectively”
t0“Rosa hybrida cv. Iceberg”
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