Abstract
Influence of culture media and hormone concentrations on plant regeneration from rose ( Rosa hybrida L. cv. Raktagandha) leaf segments were investigated. Leaves were excised from healthy, well-grown and mature plants. Leaf segments (4-5 mm long) were sectioned and cultured on Murashige and Skoog (1962) medium containing different concentration of growth hormones. Callus formation was most prolific (97.09%) on MS medium containing MS basal salts + 0.5 mg·L -1 BAP + 2.0 mg·L -1 2,4-D. maximum (56.67%) organogenesis or shoot differentiation was achieved on MS modified medium supplemented with 1.0 mg L-1 BAP + 0.1 mg·L -1 NAA + 10.0 mg·L -1 Adenine Sulphate. The highest percentage (93.73%) of in-vitro rooting was observed in half-strength MS basal medium containing 0.5 mg·L -1 IBA. Rooted plants were transferred in to sterilized potting mixture and grown in a greenhouse.
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Claims 3
Claim · model-written summary
MS medium with 0.5 mg·L -1 BAP and 2.0 mg·L -1 2,4-D gave the most prolific callus formation of rose leaf segments.
- Taxon
- Rosa hybrida
Rosa hybrid cultivare0 - Material
- Raktagandhae0
- Organ / stage
- leaf segmentse0
- Treatment
- MS medium containing MS basal salts + 0.5 mg·L -1 BAP + 2.0 mg·L -1 2,4-De1
- Outcome
- Callus formatione1
- Direction
- most prolifice1
- Value
- 97.09%e1
- Setting
- In Vitro Culturee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Influence of culture media and hormone concentrations on plant regeneration from rose ( Rosa hybrida L. cv. Raktagandha) leaf segments were investigated”
e1“Callus formation was most prolific (97.09%) on MS medium containing MS basal salts + 0.5 mg·L -1 BAP + 2.0 mg·L -1 2,4-D”
e5“from In Vitro Culture of Rose Leaf Segments”
Claim · model-written summary
MS modified medium with 1.0 mg L-1 BAP, 0.1 mg·L -1 NAA and 10.0 mg·L -1 Adenine Sulphate gave the maximum organogenesis or shoot differentiation of rose leaf segments.
- Taxon
- Rosa hybridaRosa hybrid cultivare0
- Material
- Raktagandhae0
- Organ / stage
- leaf segmentse0
- Treatment
- MS modified medium supplemented with 1.0 mg L-1 BAP + 0.1 mg·L -1 NAA + 10.0 mg·L -1 Adenine Sulphatee2
- Outcome
- organogenesis or shoot differentiatione2
- Direction
- maximume2
- Value
- 56.67%e2
- Setting
- In Vitro Culturee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Influence of culture media and hormone concentrations on plant regeneration from rose ( Rosa hybrida L. cv. Raktagandha) leaf segments were investigated”
e2“maximum (56.67%) organogenesis or shoot differentiation was achieved on MS modified medium supplemented with 1.0 mg L-1 BAP + 0.1 mg·L -1 NAA + 10.0 mg·L -1 Adenine Sulphate”
e5“from In Vitro Culture of Rose Leaf Segments”
Claim · model-written summary
Half-strength MS basal medium with 0.5 mg·L -1 IBA gave the highest in-vitro rooting of rose plantlets.
- Taxon
- Rosa hybridaRosa hybrid cultivare0
- Material
- Raktagandhae0
- Treatment
- half-strength MS basal medium containing 0.5 mg·L -1 IBAe3
- Outcome
- in-vitro rootinge3
- Direction
- higheste3
- Value
- 93.73%e3
- Setting
- In Vitro Culturee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Influence of culture media and hormone concentrations on plant regeneration from rose ( Rosa hybrida L. cv. Raktagandha) leaf segments were investigated”
e3“The highest percentage (93.73%) of in-vitro rooting was observed in half-strength MS basal medium containing 0.5 mg·L -1 IBA”
e5“from In Vitro Culture of Rose Leaf Segments”
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