Abstract
Tobacco mesophyll protoplasts were previously shown to respond to naphthaleneacetic acid by modifying their transmembrane potential difference. In the present work, evacuolated protoplasts were used to show that this response resides only at the plasmalemma. This electrical response was investigated by using polyclonal antibodies directed against plasma membrane antigens presumably involved in the reception and transduction of the auxin signal. An IgG fraction from an antiserum directed against the membrane auxin-binding protein from maize coleoptile completely inhibited the naphthaleneacetic acid-induced response of tobacco protoplasts. The suppression of the auxin-induced variation in the transmembrane potential difference by an IgG preparation directed against the plasmalemma ATPase from yeast demonstrated the involvement of the ATPase in the electrical response. Variation induced by fusicoccin in the transmembrane potential difference of tobacco protoplasts was unaffected by the anti-auxin-binding protein IgG fraction but was completely suppressed by the anti-ATPase IgG preparation. These results demonstrate the presence of a membrane receptor for auxin at the plasmalemma, the binding of the hormone to this receptor leading to the activation of the proton-pumping ATPase. They also show that at least the primary steps of activation by naphthaleneacetic acid are distinct from those of the fusicoccin-induced response.
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Claims 8
Claim · model-written summary
The auxin electrical response resided at the plasmalemma.
- Taxon
- Tobacco
Nicotiana tabacumt0 - Outcome
- response resides only at the plasmalemmae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“evacuolated protoplasts were used to show that this response resides only at the plasmalemma”
t0“tobacco”
Claim · model-written summary
Anti-auxin-binding-protein IgG fully blocked the naphthaleneacetic acid response.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- An IgG fraction from an antiserum directed against the membrane auxin-binding protein from maize coleoptilee0
- Gene
- membrane auxin-binding proteine0
- Outcome
- naphthaleneacetic acid-induced responsee0
- Direction
- completely inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An IgG fraction from an antiserum directed against the membrane auxin-binding protein from maize coleoptile completely inhibited the naphthaleneacetic acid-induced response of tobacco protoplasts”
Claim · model-written summary
Anti-ATPase IgG suppressed the auxin electrical response.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- an IgG preparation directed against the plasmalemma ATPase from yeaste0
- Gene
- plasmalemma ATPasee0
- Outcome
- auxin-induced variation in the transmembrane potential differencee0
- Direction
- suppressione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The suppression of the auxin-induced variation in the transmembrane potential difference by an IgG preparation directed against the plasmalemma ATPase from yeast”
t0“tobacco”
Claim · model-written summary
Anti-auxin-binding-protein IgG did not affect the fusicoccin electrical response.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- anti-auxin-binding protein IgG fractione0
- Outcome
- Variation induced by fusicoccin in the transmembrane potential differencee0
- Direction
- unaffectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Variation induced by fusicoccin in the transmembrane potential difference of tobacco protoplasts was unaffected by the anti-auxin-binding protein IgG fraction”
Claim · model-written summary
Anti-ATPase IgG completely suppressed the fusicoccin response.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- anti-ATPase IgG preparatione0
- Outcome
- fusicoccin-induced responsee1
- Direction
- completely suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was completely suppressed by the anti-ATPase IgG preparation”
t0“tobacco”
e1“the fusicoccin-induced response”
Claim · model-written summary
The authors infer a membrane auxin receptor at the plasmalemma.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- presence of a membrane receptor for auxin at the plasmalemmae0
- Qualifier
- auxine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results demonstrate the presence of a membrane receptor for auxin at the plasmalemma”
t0“tobacco”
Claim · model-written summary
Auxin binding to its receptor activates proton-pumping ATPase.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- proton-pumping ATPasee0
- Outcome
- activation of the proton-pumping ATPasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the binding of the hormone to this receptor leading to the activation of the proton-pumping ATPase”
t0“tobacco”
Claim · model-written summary
Initial activation by naphthaleneacetic acid differs from fusicoccin-induced activation.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- primary steps of activation by naphthaleneacetic acid are distinct from those of the fusicoccin-induced responsee0
- Qualifier
- naphthaleneacetic acide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the primary steps of activation by naphthaleneacetic acid are distinct from those of the fusicoccin-induced response”
t0“tobacco”
The same questions in other papers
class · molecular: metabolites · Nicotiana tabacum (tobacco)Positive and negative results
4 papers in the same direction as this paper · 9 in the opposite direction
- positive
- no effect
- positive
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.