Abstract
Detection and quantification of Macrophomina phaseolina , causal agent of charcoal/dry root rot disease in many crop plants, was carried out using the ELISA serological technique. Polyclonal antisera raised to water soluble extracts of mycelium, the residual water insoluble mycelial materials or ribosomal proteins were evaluated for specificity and cross‐reactivity with 16 common soil fungi by ODD and DAS–ELISA. Soluble and cell wall antisera exhibited strong cross‐reactivity with most of the fungal isolates. Ribosomal antibodies were less reactive to common soil fungi except Fusarium oxysporum f.sp. ciceri . Mycelial antigens of M. phaseolina on chickpea roots were detectable with DAS–ELISA at a minimum concentration of 10 ng g −1 at 1 : 100 root : buffer dilution. Quantitative estimation of M. phaseolina on roots was evaluated by ELISA under different temperatures and moisture conditions, and in soil amended with a potential antagonist ( Trichoderma harzianum 25‐92). A significant reduction in ELISA values was observed in T. harzianum ‐amended treatments. This method may be useful for detection and rapid screening of M. phaseolina under different environmental conditions.
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Claims 4
Claim · model-written summary
Mycelial antigens of M. phaseolina on chickpea roots were detectable by DAS–ELISA at a minimum concentration of 10 ng g −1.
- Taxon
- chickpea
Cicer arietinume1 - Interactor
- M. phaseolina
Macrophomina phaseolinae1pathogen - Outcome
- detectablee1
- Value
- 10 ng g −1e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Mycelial antigens of M. phaseolina on chickpea roots were detectable with DAS–ELISA at a minimum concentration of 10 ng g −1”
Claim · model-written summary
ELISA values were significantly reduced in T. harzianum-amended treatments compared with the untreated soil.
- Taxon
- chickpeaCicer arietinume5
- Treatment
- soil amended with a potential antagoniste4
- Interactor
- M. phaseolinaMacrophomina phaseolinae5pathogen
- Second interactor
- T. harzianum
Trichoderma harzianume2biocontrol_agent - Outcome
- ELISA valuese2
- Direction
- reductione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“A significant reduction in ELISA values was observed in T. harzianum ‐amended treatments”
e4“in soil amended with a potential antagonist”
e5“Mycelial antigens of M. phaseolina on chickpea roots”
Claim · model-written summary
Soluble and cell wall antisera showed strong cross-reactivity with most of the fungal isolates tested.
- Taxon
- chickpeaCicer arietinume5
- Outcome
- cross‐reactivitye3
- Comparator
- most of the fungal isolatese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Soluble and cell wall antisera exhibited strong cross‐reactivity with most of the fungal isolates”
e5“Mycelial antigens of M. phaseolina on chickpea roots”
Claim · model-written summary
Ribosomal antibodies were less reactive to common soil fungi, except Fusarium oxysporum f.sp. ciceri.
- Taxon
- chickpeaCicer arietinume5
- Outcome
- reactivee4
- Direction
- lesse4
- Comparator
- common soil fungie4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Ribosomal antibodies were less reactive to common soil fungi except Fusarium oxysporum f.sp. ciceri”
e5“Mycelial antigens of M. phaseolina on chickpea roots”
The same questions in other papers
biocontrol · Macrophomina phaseolina · Cicer arietinum (chickpea)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.