Abstract
Hexokinase is the first enzyme in the hexose assimilation pathway; it acts as a sensor for plant sugar responses, and it is also important in determining the fruit sugar levels. The full-length cDNA of a hexokinase gene was isolated from loquat through reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends, which was designated as EjHXK1. EjHXK1 is 1,839 bp long and contains an entire open reading frame encoding 497 amino acids. The predicted protein of EjHXK1 shares 72%-81% similarity with other plant hexokinases. Phylogeny analysis indicated that EjHXK1 is closely related to maize and rice hexokinases. Transient expression of the 35S: EjHXK1-GFP fusion protein was observed on the cell membrane and cytoplasm. Real-time RT-PCR indicated that EjHXK1 is expressed in loquat leaves, stems, flowers, and fruits. EjHXK1 transcripts were higher during early fruit development, but decreases before maturation, which is consistent with hexokinase enzyme activity during fruit development and conducive for hexose accumulation in mature fruits. These results imply that EjHXK1 may play important roles in the regulation of sugar flux during fruit ripening.
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Claims 10
Claim · model-written summary
A full-length loquat hexokinase cDNA was isolated and named EjHXK1.
- Taxon
- loquat
Eriobotrya japonicae0 - Gene
- EjHXK1e0
- Outcome
- full-length cDNA of a hexokinase genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The full-length cDNA of a hexokinase gene was isolated from loquat through reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends, which was designated as EjHXK1.”
Claim · model-written summary
EjHXK1 is 1,839 bp long.
- Taxon
- LoquatEriobotrya japonicat0
- Gene
- EjHXK1e0
- Outcome
- EjHXK1 is 1,839 bp longe0
- Value
- 1,839 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Loquat (Eriobotrya japonica Lindl.)”
e0“EjHXK1 is 1,839 bp long”
Claim · model-written summary
EjHXK1 encodes 497 amino acids.
- Taxon
- LoquatEriobotrya japonicat0
- Outcome
- open reading frame encoding 497 amino acidse0
- Value
- 497 amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Loquat (Eriobotrya japonica Lindl.)”
e0“contains an entire open reading frame encoding 497 amino acids”
Claim · model-written summary
Predicted EjHXK1 protein shares 72%-81% similarity with other plant hexokinases.
- Taxon
- LoquatEriobotrya japonicat0
- Gene
- EjHXK1e0
- Outcome
- similarity with other plant hexokinasese0
- Value
- 72%-81%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Loquat (Eriobotrya japonica Lindl.)”
e0“The predicted protein of EjHXK1 shares 72%-81% similarity with other plant hexokinases.”
Claim · model-written summary
EjHXK1 is phylogenetically close to maize and rice hexokinases.
- Taxon
- LoquatEriobotrya japonicat0
- Gene
- EjHXK1e0
- Outcome
- closely related to maize and rice hexokinasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Loquat (Eriobotrya japonica Lindl.)”
e0“Phylogeny analysis indicated that EjHXK1 is closely related to maize and rice hexokinases.”
Claim · model-written summary
Transient EjHXK1-GFP expression localized to the cell membrane and cytoplasm.
- Taxon
- LoquatEriobotrya japonicat0
- Gene
- EjHXK1-GFPe0
- Outcome
- observed on the cell membrane and cytoplasme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Loquat (Eriobotrya japonica Lindl.)”
e0“Transient expression of the 35S: EjHXK1-GFP fusion protein was observed on the cell membrane and cytoplasm.”
Claim · model-written summary
EjHXK1 is expressed in loquat leaves, stems, flowers, and fruits.
- Taxon
- loquatEriobotrya japonicae0
- Organ / stage
- leaves, stems, flowers, and fruitse0
- Gene
- EjHXK1e0
- Outcome
- expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Real-time RT-PCR indicated that EjHXK1 is expressed in loquat leaves, stems, flowers, and fruits.”
Claim · model-written summary
EjHXK1 transcripts were higher early in fruit development and decreased before maturation.
- Taxon
- LoquatEriobotrya japonicat0
- Organ / stage
- fruite0
- Gene
- EjHXK1e0
- Outcome
- EjHXK1 transcriptse0
- Direction
- highere0
- Qualifier
- decreases before maturatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Loquat (Eriobotrya japonica Lindl.)”
e0“EjHXK1 transcripts were higher during early fruit development, but decreases before maturation”
Claim · model-written summary
Fruit-development transcript pattern was consistent with hexokinase activity and conducive to hexose accumulation in mature fruit.
- Taxon
- LoquatEriobotrya japonicat0
- Organ / stage
- fruite0
- Gene
- hexokinasee0
- Outcome
- hexose accumulation in mature fruitse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Loquat (Eriobotrya japonica Lindl.)”
e0“which is consistent with hexokinase enzyme activity during fruit development and conducive for hexose accumulation in mature fruits”
Claim · model-written summary
The authors infer that EjHXK1 may regulate sugar flux during fruit ripening.
- Taxon
- LoquatEriobotrya japonicat0
- Gene
- EjHXK1e0
- Outcome
- regulation of sugar flux during fruit ripeninge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Loquat (Eriobotrya japonica Lindl.)”
e0“EjHXK1 may play important roles in the regulation of sugar flux during fruit ripening”