Paper · 1977 · PLANT PHYSIOLOGY

Nitrite Reduction in Reconstituted and Whole Spinach Chloroplasts during Carbon Dioxide Reduction

Zvi Plaut; Klaus Lendzian; James A. Bassham

4
claims
7
verbatim quotes
21
citations
1
research areas
✓
Open access

Abstract

Nitrite reduction in either whole, isolated spinach chloroplasts (Spinacia oleracea L.) or in reconstituted spinach chloroplasts is stimulated by a short period of photosynthetic CO(2) fixation in the light prior to nitrite addition. With reconstituted chloroplasts, a similar stimulation can be obtained in nitrite reduction without CO(2) fixation by the addition of dihydroxyacetone phosphate or fructose 6-phosphate. Specific intermediate metabolites of the photosynthetic carbon reduction cycle may have a regulatory role in nitrite reduction in chloroplasts in the light.

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Claims 4

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Prior photosynthetic CO(2) fixation stimulates nitrite reduction in whole and reconstituted spinach chloroplasts.

Organ / stage
chloroplastse0
Treatment
a short period of photosynthetic CO(2) fixation in the light prior to nitrite additione0
Outcome
Nitrite reductione0
Direction
stimulatede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Nitrite reduction in either whole, isolated spinach chloroplasts (Spinacia oleracea L.) or in reconstituted spinach chloroplasts is stimulated by a short period of photosynthetic CO(2) fixation in the light prior to nitrite addition”

✓ located verbatim in the source · abstract, characters 0–232 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Dihydroxyacetone phosphate stimulates nitrite reduction in reconstituted chloroplasts without CO(2) fixation.

Organ / stage
reconstituted chloroplastse0
Treatment
addition of dihydroxyacetone phosphatee0
Outcome
nitrite reductione0
Direction
stimulatione0
Comparator
without CO(2) fixatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“With reconstituted chloroplasts, a similar stimulation can be obtained in nitrite reduction without CO(2) fixation by the addition of dihydroxyacetone phosphate or fructose 6-phosphate”

✓ located verbatim in the source · abstract, characters 234–418 · show in text ↑
t0

“Spinach”

✓ located verbatim in the source · title, characters 45–52 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Fructose 6-phosphate stimulates nitrite reduction in reconstituted chloroplasts without CO(2) fixation.

Organ / stage
reconstituted chloroplastse0
Treatment
fructose 6-phosphatee0
Outcome
nitrite reductione0
Direction
stimulatione0
Comparator
without CO(2) fixatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“With reconstituted chloroplasts, a similar stimulation can be obtained in nitrite reduction without CO(2) fixation by the addition of dihydroxyacetone phosphate or fructose 6-phosphate”

✓ located verbatim in the source · abstract, characters 234–418 · show in text ↑
t0

“Spinach”

✓ located verbatim in the source · title, characters 45–52 · show in text ↑
Mechanismhypothesisoriginal resultbasis n/a

Claim · model-written summary

Photosynthetic carbon reduction intermediates may regulate chloroplast nitrite reduction in light.

Compound
intermediate metabolites of the photosynthetic carbon reduction cyclee0
Outcome
nitrite reductione0
Qualifier
may have a regulatory rolee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Specific intermediate metabolites of the photosynthetic carbon reduction cycle may have a regulatory role in nitrite reduction in chloroplasts in the light”

✓ located verbatim in the source · abstract, characters 420–575 · show in text ↑
t0

“Spinach”

✓ located verbatim in the source · title, characters 45–52 · show in text ↑

nitrite · molecular: metabolites · Spinacia oleracea (spinach)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. no effect
See all 2 papers →

co2 · molecular: metabolites · Spinacia oleracea (spinach)Positive and negative results

6 papers: 3 positive, 3 negative or no effect

2 papers in the same direction as this paper · 3 in the opposite direction

  1. no effect
  2. no effect
  3. negative

2 papers more in this group

See all 6 papers →

light · molecular: metabolites · Spinacia oleracea (spinach)Positive and negative results

29 papers: 16 positive, 13 negative or no effect

15 papers in the same direction as this paper · 13 in the opposite direction

  1. negative
  2. no effect
  3. negative

25 papers more in this group

See all 29 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.