Abstract
Arabidopsis thaliana plants (wild type accessions Col and N1438) were grown in nutrient solution for 34 d with or without 50 mM NaCl. Salt stress inhibited plant growth rate more in Col than in N1438 and a decrease in K+, Ca2+ and nitrogen contents was observed in both accessions. NaCl diminished accumulation of malate, fumarate and citrate only in Col accession. To measure the effect of NaCl on transcript level of superoxide dismutase (SOD) isoforms and copper chaperone for SOD genes, a semi-quantitative polymerase chain reaction (RT-PCR) method was developed using cDNA normalized against the EF1a gene in parallel with quantitative real time RT-PCR (Q-PCR) technique. Both methods gave the same results. The abundance of transcripts of the three genes coding for Cu/Zn-SOD responded similarly to NaCl in both accessions: CSD1 gene was overexpressed, and CSD2 and CSD3 genes were repressed. However, the genes coding for Fe-SOD (FSD1), Mn-SOD (MSD1) and Cu-chaperone for SOD (CCS) responded to NaCl differently in Col and N1438: the former gene was overexpressed in Col and repressed in N1438, and the opposite behaviour was observed for the latter two genes.
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Claims 7
Claim · model-written summary
NaCl inhibited growth rate more in Col than in N1438.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Material
- Cole0
- Treatment
- 50 mM NaCle0
- Outcome
- plant growth ratee0
- Direction
- inhibitede0
- Comparator
- N1438e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana plants (wild type accessions Col and N1438) were grown in nutrient solution for 34 d with or without 50 mM NaCl. Salt stress inhibited plant growth rate more in Col than in N1438”
Claim · model-written summary
NaCl decreased K+, Ca2+ and nitrogen contents in both Arabidopsis accessions.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- Col than in N1438e0
- Treatment
- Salt stresse0
- Outcome
- K+, Ca2+ and nitrogen contentse0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Salt stress inhibited plant growth rate more in Col than in N1438 and a decrease in K+, Ca2+ and nitrogen contents was observed in both accessions.”
t0“Arabidopsis thaliana”
Claim · model-written summary
NaCl diminished malate, fumarate and citrate accumulation only in Col.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- Cole0
- Treatment
- NaCle0
- Outcome
- accumulation of malate, fumarate and citratee0
- Direction
- diminishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NaCl diminished accumulation of malate, fumarate and citrate only in Col accession.”
t0“Arabidopsis thaliana”
Claim · model-written summary
Semi-quantitative RT-PCR and Q-PCR gave the same results for SOD and copper chaperone transcripts.
Verbatim
e0“a semi-quantitative polymerase chain reaction (RT-PCR) method was developed using cDNA normalized against the EF1a gene in parallel with quantitative real time RT-PCR (Q-PCR) technique. Both methods gave the same results.”
Claim · model-written summary
NaCl increased CSD1 transcripts and repressed CSD2 and CSD3 transcripts in both accessions.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- NaCle0
- Gene
- CSD1e0
- Outcome
- abundance of transcriptse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The abundance of transcripts of the three genes coding for Cu/Zn-SOD responded similarly to NaCl in both accessions: CSD1 gene was overexpressed, and CSD2 and CSD3 genes were repressed.”
t0“Arabidopsis thaliana”
Claim · model-written summary
NaCl increased FSD1 transcript in Col and repressed it in N1438.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- NaCle1
- Gene
- FSD1e1
- Outcome
- FSD1e1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the former gene was overexpressed in Col and repressed in N1438”
e1“the genes coding for Fe-SOD (FSD1), Mn-SOD (MSD1) and Cu-chaperone for SOD (CCS) responded to NaCl differently in Col and N1438”
t0“Arabidopsis thaliana”
Claim · model-written summary
NaCl repressed MSD1 and CCS transcripts in Col and increased them in N1438.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- NaCle1
- Gene
- MSD1e1
- Outcome
- MSD1e1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the opposite behaviour was observed for the latter two genes”
e1“the genes coding for Fe-SOD (FSD1), Mn-SOD (MSD1) and Cu-chaperone for SOD (CCS) responded to NaCl differently in Col and N1438”
t0“Arabidopsis thaliana”
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