Abstract
A pea (Pisum sativum) DNA fragment (termed MB3) was isolated by differential display of cDNAs obtained from total leaf RNA of ultraviolet B (UV-B) radiation-treated plants. Longer cDNAs were cloned by rapid amplification of cDNA ends in the 3' to 5' direction. Three different, but very similar, cDNAs were cloned, sadA, sadB, and sadC, the major difference between them being a 36-bp deletion in the coding region of sadB. Southern blotting confirmed the occurrence of at least three genes in the pea genome. Database comparisons of the SAD protein sequences revealed high identity (46%) and similarity (77%) with a putative tomato (Lycopersicon esculentum) short-chain alcohol dehydrogenase. Very low levels of UV-B radiation (the biologically effective radiation normalized to 300 nm = 0.08 W m(-2)) was shown to up-regulate expression, a dose considerably lower than that needed to induce expression of the well-known UV-B defensive chalcone synthase and phenylalanine ammonia lyase genes. RNase protection assay revealed that primarily sadA and sadC mRNA accumulation was enhanced by UV-B. In addition to UV-B irradiation, ozone fumigation, wounding, aluminum stress, and salt stress induced increased transcript levels of the sad genes in pea.
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Claims 9
Claim · model-written summary
Three similar pea cDNAs, sadA, sadB and sadC, were cloned.
- Taxon
- pea
Lathyrus oleraceuse0e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three different, but very similar, cDNAs were cloned, sadA, sadB, and sadC, the major difference between them being a 36-bp deletion in the coding region of sadB.”
e1“A pea (Pisum sativum) DNA fragment (termed MB3) was isolated”
Claim · model-written summary
At least three genes occurred in the pea genome.
- Taxon
- peaLathyrus oleraceuse0e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Southern blotting confirmed the occurrence of at least three genes in the pea genome.”
e1“A pea (Pisum sativum) DNA fragment (termed MB3) was isolated”
Claim · model-written summary
Pea SAD protein sequences had 46% identity and 77% similarity to a putative tomato short-chain alcohol dehydrogenase.
- Taxon
- peaLathyrus oleraceuse0e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SAD protein sequences revealed high identity (46%) and similarity (77%) with a putative tomato (Lycopersicon esculentum) short-chain alcohol dehydrogenase.”
e1“A pea (Pisum sativum) DNA fragment (termed MB3) was isolated”
Claim · model-written summary
Very low UV-B radiation up-regulated expression in pea.
- Taxon
- peaLathyrus oleraceuse0e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Very low levels of UV-B radiation (the biologically effective radiation normalized to 300 nm = 0.08 W m(-2)) was shown to up-regulate expression”
e1“A pea (Pisum sativum) DNA fragment (termed MB3) was isolated”
Claim · model-written summary
UV-B enhanced sadA and sadC mRNA accumulation in pea.
- Taxon
- peaLathyrus oleraceuse0e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“primarily sadA and sadC mRNA accumulation was enhanced by UV-B.”
e1“A pea (Pisum sativum) DNA fragment (termed MB3) was isolated”
Claim · model-written summary
Ozone fumigation increased sad transcript levels in pea.
- Taxon
- peaLathyrus oleraceuse0e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ozone fumigation, wounding, aluminum stress, and salt stress induced increased transcript levels of the sad genes in pea.”
e1“A pea (Pisum sativum) DNA fragment (termed MB3) was isolated”
Claim · model-written summary
Wounding increased sad transcript levels in pea.
- Taxon
- peaLathyrus oleraceuse0e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ozone fumigation, wounding, aluminum stress, and salt stress induced increased transcript levels of the sad genes in pea.”
e1“A pea (Pisum sativum) DNA fragment (termed MB3) was isolated”
Claim · model-written summary
Aluminum stress increased sad transcript levels in pea.
- Taxon
- peaLathyrus oleraceuse0e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ozone fumigation, wounding, aluminum stress, and salt stress induced increased transcript levels of the sad genes in pea.”
e1“A pea (Pisum sativum) DNA fragment (termed MB3) was isolated”
Claim · model-written summary
Salt stress increased sad transcript levels in pea.
- Taxon
- peaLathyrus oleraceuse0e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ozone fumigation, wounding, aluminum stress, and salt stress induced increased transcript levels of the sad genes in pea.”
e1“A pea (Pisum sativum) DNA fragment (termed MB3) was isolated”
The same questions in other papers
radiation · molecular: expression · Lathyrus oleraceus (pea)Positive and negative results
2 in the opposite direction
- negative
- negative
uv · molecular: expression · Lathyrus oleraceus (pea)Positive and negative results
3 papers in the same direction as this paper · 5 in the opposite direction
- negative
- negative
- negative
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.