Abstract
The effect of supplemental UV-B radiation on gene expression for three photosynthetic proteins (chlorophyll a/b-binding protein, D1 polypeptide of PS II and RUBISCO) and on flavonoid composition has been investigated in expanded leaves and in apical buds of pea seedlings. In the expanded third leaves, UV-B caused a decrease in nuclear-encoded Lhcb and RbcS transcript levels within 2 d of UV-B treatment and a later decline in the SSU and LHCB polypeptides. The effect of UV-B on the chloroplast-encoded rbcL and psbA genes was more complex. Levels of LSU and D1 initially appeared to be regulated at the translational and/or post-translational level and only later in the UV-B treatment, at the level of mRNA abundance. UV-B also accelerated the degradation of the D1 polypeptide. For all genes studied the UV-B-induced inhibition of transcripts was greater in leaves than in the apical buds, indicating that the bud is less sensitive to UV-B damage. The mRNA transcript levels for chalcone synthase (chs) were also measured. In leaves, chs RNA transcripts were initially present in low amounts and exposure to supplemental UV-B resulted in a transient increase in the level of these transcripts. In contrast, chs transcripts in the apical buds were initially present at high levels, with UV-B exposure resulting in a slow progressive decline in transcript levels. These results indicate that the response to UV-B is complex and is dependent on the organ studied.
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Claims 7
Claim · model-written summary
UV-B decreased nuclear-encoded Lhcb and RbcS transcript levels in expanded third leaves within 2 d of treatment.
- Taxon
- pea
Lathyrus oleraceuse0 - Organ / stage
- expanded third leavese1
- Treatment
- UV-Be1
- Gene
- Lhcb and RbcSe1
- Outcome
- Lhcb and RbcS transcript levelse1
- Direction
- decreasee1
- Qualifier
- within 2 d of UV-B treatmente1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in expanded leaves and in apical buds of pea seedlings”
e1“In the expanded third leaves, UV-B caused a decrease in nuclear-encoded Lhcb and RbcS transcript levels within 2 d of UV-B treatment and a later decline in the SSU and LHCB polypeptides”
Claim · model-written summary
UV-B later reduced the SSU and LHCB polypeptides in expanded third leaves.
- Taxon
- peaLathyrus oleraceuse0
- Organ / stage
- expanded third leavese1
- Treatment
- UV-Be1
- Outcome
- SSU and LHCB polypeptidese1
- Direction
- declinee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in expanded leaves and in apical buds of pea seedlings”
e1“In the expanded third leaves, UV-B caused a decrease in nuclear-encoded Lhcb and RbcS transcript levels within 2 d of UV-B treatment and a later decline in the SSU and LHCB polypeptides”
Claim · model-written summary
LSU and D1 levels were initially regulated at the translational or post-translational level and only later at the level of mRNA abundance under UV-B.
- Taxon
- peaLathyrus oleraceuse0
- Gene
- LSU and D1e2
- Outcome
- Levels of LSU and D1e2
- Qualifier
- translational and/or post-translational levele2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in expanded leaves and in apical buds of pea seedlings”
e2“Levels of LSU and D1 initially appeared to be regulated at the translational and/or post-translational level and only later in the UV-B treatment, at the level of mRNA abundance”
Claim · model-written summary
UV-B accelerated the degradation of the D1 polypeptide.
- Taxon
- peaLathyrus oleraceuse0
- Treatment
- UV-Be3
- Gene
- D1 polypeptidee3
- Outcome
- degradation of the D1 polypeptidee3
- Direction
- acceleratede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in expanded leaves and in apical buds of pea seedlings”
e3“UV-B also accelerated the degradation of the D1 polypeptide”
Claim · model-written summary
The UV-B-induced inhibition of transcripts was greater in leaves than in apical buds, indicating that buds are less sensitive to UV-B damage.
- Taxon
- peaLathyrus oleraceuse0
- Organ / stage
- leavese4
- Treatment
- UV-Be4
- Outcome
- inhibition of transcriptse4
- Direction
- greatere4
- Comparator
- apical budse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in expanded leaves and in apical buds of pea seedlings”
e4“For all genes studied the UV-B-induced inhibition of transcripts was greater in leaves than in the apical buds, indicating that the bud is less sensitive to UV-B damage”
Claim · model-written summary
UV-B exposure transiently increased chs RNA transcript levels in leaves.
- Taxon
- peaLathyrus oleraceuse0
- Organ / stage
- leavese5
- Treatment
- supplemental UV-Be5
- Gene
- chse5
- Outcome
- chs RNA transcriptse5
- Direction
- increasee5
- Qualifier
- transiente5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in expanded leaves and in apical buds of pea seedlings”
e5“In leaves, chs RNA transcripts were initially present in low amounts and exposure to supplemental UV-B resulted in a transient increase in the level of these transcripts”
Claim · model-written summary
UV-B exposure slowly and progressively declined chs transcript levels in apical buds, which were initially high.
- Taxon
- peaLathyrus oleraceuse0
- Organ / stage
- apical budse6
- Treatment
- UV-B exposuree6
- Gene
- chse6
- Outcome
- chs transcriptse6
- Direction
- declinee6
- Qualifier
- slow progressivee6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in expanded leaves and in apical buds of pea seedlings”
e6“In contrast, chs transcripts in the apical buds were initially present at high levels, with UV-B exposure resulting in a slow progressive decline in transcript levels”
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See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.