Abstract
Light‐harvesting complex II chlorophyll a/b ‐binding protein (Lhcb) mRNA levels are differentially affected by ultraviolet‐B radiation (UV‐B, 280‐320 nm) at different stages of development of pea ( Pisum sativum L. cv. Feltham first) seedlings. Addition of UV‐B radiation to the light periods of diurnal cycles of white light resulted in reduction of total Lhcb mRNAs in green leaves but a transient increase in etiolated buds. The aims of this study were to determine the stage during de‐etiolation at which supplementary UV‐B began to inhibit Lhcb gene expression, and to determine whether differential regulation of individual Lhcb genes could explain the differential response to supplementary UV‐B at different developmental stages. All seven Lhcb mRNAs were shown to increase in etiolated buds transferred to a diurnal cycle with supplementary UV‐B during the light periods, but were greatly reduced in green leaves given the same treatment. Therefore, the different responses of total Lhcb mRNA levels to UV‐B radiation in green leaves and etiolated buds are not primarily due to the expression of different members of the Lhcb gene family at different developmental stages. However, the Lhcb genes could be divided into two groups based on their sensitivities to UV‐B. Transcripts from the three genes, Lhcb1 * 2 , Lhcb1 * 3 and Lhcb1 * 5 , which were undetectable in dark‐grown etiolated buds, exhibited stronger responses to supplementary UV‐B in green leaves than the four genes, Lhcb1 * 1 , Lhcb1 * 4 , Lhcb2 * 1 and Lhcb3 * 1 , which showed low levels of initial transcript accumulation in dark‐grown etiolated buds. The effect of UV‐B on Lhcb mRNA levels were, however, correlated with chlorophyll content, suggesting that the developmental stage of chloroplasts may be important in determining the responses of the Lhcb genes to supplementary UV‐B radiation in pea seedlings.
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Claims 6
Claim · model-written summary
All seven Lhcb mRNAs increased in etiolated buds under supplementary UV-B.
- Taxon
- pea
Lathyrus oleraceust0 - Organ / stage
- etiolated budse0
- Treatment
- supplementary UV‐Be0
- Outcome
- Lhcb mRNAse0
- Direction
- increasee0
- Value
- sevene0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All seven Lhcb mRNAs were shown to increase in etiolated buds transferred to a diurnal cycle with supplementary UV‐B during the light periods”
t0“pea (Pisum sativum)”
Claim · model-written summary
All seven Lhcb transcripts were greatly reduced in green leaves under the same UV-B treatment.
- Taxon
- peaLathyrus oleraceust0
- Organ / stage
- green leavese0
- Treatment
- supplementary UV‐Be0
- Outcome
- Lhcb mRNAse0
- Direction
- greatly reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All seven Lhcb mRNAs were shown to increase in etiolated buds transferred to a diurnal cycle with supplementary UV‐B during the light periods, but were greatly reduced in green leaves given the same treatment”
t0“pea (Pisum sativum)”
Claim · model-written summary
Three Lhcb1 genes showed stronger UV-B responses in green leaves than four other Lhcb genes.
- Taxon
- peaLathyrus oleraceust0
- Gene
- Lhcb1 * 2 , Lhcb1 * 3 and Lhcb1 * 5e0
- Outcome
- responses to supplementary UV‐Be0
- Direction
- strongere0
- Comparator
- Lhcb1 * 1 , Lhcb1 * 4 , Lhcb2 * 1 and Lhcb3 * 1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lhcb1 * 2 , Lhcb1 * 3 and Lhcb1 * 5 , which were undetectable in dark‐grown etiolated buds, exhibited stronger responses to supplementary UV‐B in green leaves than the four genes, Lhcb1 * 1 , Lhcb1 * 4 , Lhcb2 * 1 and Lhcb3 * 1”
t0“pea (Pisum sativum)”
Claim · model-written summary
The three more UV-B sensitive genes were undetectable in dark-grown etiolated buds.
- Taxon
- peaLathyrus oleraceust0
- Organ / stage
- dark‐grown etiolated budse0
- Gene
- Lhcb1 * 2 , Lhcb1 * 3 and Lhcb1 * 5e0
- Outcome
- undetectablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lhcb1 * 2 , Lhcb1 * 3 and Lhcb1 * 5 , which were undetectable in dark‐grown etiolated buds”
t0“pea (Pisum sativum)”
Claim · model-written summary
Four less sensitive genes had low initial transcript accumulation in dark-grown etiolated buds.
- Taxon
- peaLathyrus oleraceust0
- Organ / stage
- dark‐grown etiolated budse0
- Gene
- Lhcb1 * 1 , Lhcb1 * 4 , Lhcb2 * 1 and Lhcb3 * 1e0
- Outcome
- low levels of initial transcript accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lhcb1 * 1 , Lhcb1 * 4 , Lhcb2 * 1 and Lhcb3 * 1 , which showed low levels of initial transcript accumulation in dark‐grown etiolated buds”
t0“pea (Pisum sativum)”
Claim · model-written summary
UV-B effects on Lhcb mRNAs correlated with chlorophyll content.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- UV‐Be0
- Compound
- chlorophylle0
- Outcome
- Lhcb mRNA levelse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effect of UV‐B on Lhcb mRNA levels were, however, correlated with chlorophyll content”
t0“pea (Pisum sativum)”
The same questions in other papers
uv · molecular: metabolites · Lathyrus oleraceus (pea)Positive and negative results
6 papers in the same direction as this paper · 11 in the opposite direction
- no effect
- no effect
- negative
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See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.