Paper · 1968 · PLANT PHYSIOLOGY

Far-red Sensitive Dark Processes Essential for Light- and Gibberellin-induced Germination of Lettuce Seed

M. Negbi; M. Black; J. D. Bewley

6
claims
12
verbatim quotes
31
citations
1
research areas
✓
Open access

Abstract

The action of prolonged far-red on seed germination was studied in Lactuca sativa L. var. Grand Rapids. Exposure of imbibed seeds to 6 hours far-red before the application of gibberellic acid (GA(3)) and thiourea completely prevented germination. Using GA(3), this far-red was effective after the sixth hour of imbibition. At 6, 12, and 18 hours of imbibition equal durations of far-red had equal effects. The kinetics of far-red action was investigated: it was found that although far-red for several hours, irrespective of the energy level, was needed for maximum inhibition, shorter durations (15 and 30 mins) were also appreciably effective provided they were followed by several hours darkness before the supply of GA(3). This is taken to indicate the existence of labile product(s) of the action of a far-red sensitive pigment. Evidence is provided for the existence of promotive dark processes controlled by this pigment, which are essential for germination whether triggered by GA(3), thiourea or red-light. A model for the operation of the pigment system is proposed and its role in the germination mechanism of this seed is discussed.

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Claims 6

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Six hours of far-red before gibberellic acid and thiourea prevented germination.

Taxon
LettuceLactuca sativat0
Treatment
6 hours far-rede0
Outcome
germinatione0
Direction
preventede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“Exposure of imbibed seeds to 6 hours far-red before the application of gibberellic acid (GA(3)) and thiourea completely prevented germination.”

✓ located verbatim in the source · abstract, characters 104–246 · show in text ↑
t0

“Lettuce Seed”

✓ located verbatim in the source · title, characters 93–105 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Far-red was effective after the sixth hour of imbibition with GA(3).

Taxon
LettuceLactuca sativat0
Outcome
far-red was effectivee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“Using GA(3), this far-red was effective after the sixth hour of imbibition.”

✓ located verbatim in the source · abstract, characters 247–322 · show in text ↑
t0

“Lettuce Seed”

✓ located verbatim in the source · title, characters 93–105 · show in text ↑
Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

Equal far-red durations had equal effects at 6, 12 and 18 hours of imbibition.

Taxon
LettuceLactuca sativat0
Outcome
equal effectse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“At 6, 12, and 18 hours of imbibition equal durations of far-red had equal effects.”

✓ located verbatim in the source · abstract, characters 323–405 · show in text ↑
t0

“Lettuce Seed”

✓ located verbatim in the source · title, characters 93–105 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Several hours of far-red were needed for maximum inhibition.

Taxon
LettuceLactuca sativat0
Outcome
maximum inhibitione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“far-red for several hours, irrespective of the energy level, was needed for maximum inhibition”

✓ located verbatim in the source · abstract, characters 482–576 · show in text ↑
t0

“Lettuce Seed”

✓ located verbatim in the source · title, characters 93–105 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Short far-red exposures were effective when followed by darkness before GA(3).

Taxon
LettuceLactuca sativat0
Outcome
appreciably effectivee0
Value
15 and 30 minse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“shorter durations (15 and 30 mins) were also appreciably effective provided they were followed by several hours darkness before the supply of GA(3).”

✓ located verbatim in the source · abstract, characters 578–726 · show in text ↑
t0

“Lettuce Seed”

✓ located verbatim in the source · title, characters 93–105 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Promotive dark processes controlled by a far-red sensitive pigment were inferred to be essential for germination.

Taxon
LettuceLactuca sativat0
Outcome
germinatione0
Qualifier
promotive dark processese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“Evidence is provided for the existence of promotive dark processes controlled by this pigment, which are essential for germination whether triggered by GA(3), thiourea or red-light.”

✓ located verbatim in the source · abstract, characters 834–1015 · show in text ↑
t0

“Lettuce Seed”

✓ located verbatim in the source · title, characters 93–105 · show in text ↑

light · growth · Lactuca sativa (garden lettuce)Positive and negative results

300 papers: 243 positive, 57 negative or no effect

56 papers in the same direction as this paper · 243 in the opposite direction

  1. positive
  2. positive
  3. positive

296 papers more in this group

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.