Abstract
The WRKY family of plant transcription factors controls several types of plant stress responses. Arabidopsis WRKY8, localized to the nucleus, is mainly induced by abscissic acid, H(2)O(2), wounding, Pseudomonas syringae and Botrytis cinerea infection, and aphid and maggot feeding. To determine its biological functions, we isolated loss-of-function T-DNA insertion mutants and generated gain-of-function overexpressing WRKY8 transgenic plants in Arabidopsis. Plants expressing the mutated WRKY8 gene showed increased resistance to P. syringae but slightly decreased resistance to B. cinerea. In contrast, transgenic plants overexpressing WRKY8 were more susceptible to P. syringae infection but more resistant to B. cinerea infection. The contrasting responses to the two pathogens were correlated with opposite effects on pathogen-induced expression of two genes; salicylic acid-regulated PATHOGENESIS-RELATED1 (PR1) and jasmonic acid-regulated PDF1.2. Therefore, our results suggest that WRKY8 is a negative regulator of basal resistance to P. syringae and positive regulator to B. cinerea.
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Claims 10
Claim · model-written summary
WRKY8 localized to the nucleus.
- Taxon
- Arabidopsis
Arabidopsise0 - Gene
- WRKY8e0
- Outcome
- localized to the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis WRKY8, localized to the nucleus”
Claim · model-written summary
WRKY8 was induced by abscissic acid, hydrogen peroxide, wounding, pathogen infections and insect feeding.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- abscissic acid, H(2)O(2), wounding, Pseudomonas syringae and Botrytis cinerea infection, and aphid and maggot feedinge0
- Gene
- WRKY8t0
- Outcome
- inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“is mainly induced by abscissic acid, H(2)O(2), wounding, Pseudomonas syringae and Botrytis cinerea infection, and aphid and maggot feeding.”
t0“WRKY8 Is Involved in Basal Defense in Arabidopsis”
Claim · model-written summary
WRKY8 mutation increased resistance to P. syringae.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- mutated WRKY8 genee0
- Interactor
- P. syringae
Pseudomonas syringaee0pathogen - Gene
- WRKY8e0
- Outcome
- resistance to P. syringaee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plants expressing the mutated WRKY8 gene showed increased resistance to P. syringae”
t0“Arabidopsis”
Claim · model-written summary
WRKY8 mutation slightly decreased resistance to B. cinerea.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- mutated WRKY8 genet1
- Interactor
- B. cinerea
Botrytis cinereae0pathogen - Gene
- WRKY8t1
- Outcome
- resistance to B. cinereae0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“slightly decreased resistance to B. cinerea”
t0“Arabidopsis”
t1“mutated WRKY8 gene”
Claim · model-written summary
WRKY8 overexpression increased susceptibility to P. syringae.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- overexpressing WRKY8e0
- Interactor
- P. syringaePseudomonas syringaee0pathogen
- Gene
- WRKY8e0
- Outcome
- susceptible to P. syringae infectione0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic plants overexpressing WRKY8 were more susceptible to P. syringae infection”
t0“Arabidopsis”
Claim · model-written summary
WRKY8 overexpression increased resistance to B. cinerea.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- overexpressing WRKY8t1
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Gene
- WRKY8t1
- Outcome
- resistant to B. cinerea infectione0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“more resistant to B. cinerea infection”
t0“Arabidopsis”
t1“transgenic plants overexpressing WRKY8”
Claim · model-written summary
Opposite pathogen responses correlated with opposite effects on PR1 expression.
- Taxon
- ArabidopsisArabidopsist0
- Interactor
- pathogense0pathogen
- Gene
- PATHOGENESIS-RELATED1 (PR1)e0
- Outcome
- pathogen-induced expressione0
- Direction
- opposite effectse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The contrasting responses to the two pathogens were correlated with opposite effects on pathogen-induced expression of two genes; salicylic acid-regulated PATHOGENESIS-RELATED1 (PR1) and jasmonic acid-regulated PDF1.2.”
t0“Arabidopsis”
Claim · model-written summary
Opposite pathogen responses correlated with opposite effects on PDF1.2 expression.
- Taxon
- ArabidopsisArabidopsist0
- Interactor
- pathogense0pathogen
- Gene
- PDF1.2e0
- Outcome
- pathogen-induced expressione0
- Direction
- opposite effectse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The contrasting responses to the two pathogens were correlated with opposite effects on pathogen-induced expression of two genes; salicylic acid-regulated PATHOGENESIS-RELATED1 (PR1) and jasmonic acid-regulated PDF1.2.”
t0“Arabidopsis”
Claim · model-written summary
Authors infer WRKY8 negatively regulates basal resistance to P. syringae.
- Taxon
- ArabidopsisArabidopsist0
- Interactor
- P. syringaePseudomonas syringaee0pathogen
- Gene
- WRKY8e0
- Outcome
- basal resistance to P. syringaee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“WRKY8 is a negative regulator of basal resistance to P. syringae”
t0“Arabidopsis”
Claim · model-written summary
Authors infer WRKY8 positively regulates basal resistance to B. cinerea.
- Taxon
- ArabidopsisArabidopsist0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Gene
- WRKY8t1
- Outcome
- positive regulator to B. cinereae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“positive regulator to B. cinerea”
t0“Arabidopsis”
t1“WRKY8 is a negative regulator”
The same questions in other papers
loss of function · Pseudomonas syringae · ArabidopsisPositive and negative results
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- no effect
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- negative
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See all 20 papers →loss of function · Botrytis cinerea · ArabidopsisPositive and negative results
15 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- positive
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See all 20 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.