Abstract
Reduced incidence of ruminant bloat was reported on legume pastures fertilized with high levels of potassium (K) or K plus nitrogen (N). Amount of soluble protein released from alfalfa ( Medicago sativa L.) into sodium maleate buffer (pH 6.9) was proposed as possible estimate of ruminant bloat potential in comparative studies. Our objective was to determine whether K and N fertilization would influence concentration of several N fractions released from alfalfa. ‘Ramsey,’ ‘Saranac,’ and ‘520’ alfalfa cultivars were fertillzed in the fall with 0 (control), 350, and 700 kg as K 2 SO 4 /ha and in the following spring with these same levels of N as NH4NO(3 4 3 × 3 factorial). The upper 10 to 15 cm of told‐bud stage alfalfa in first and second crops was analyzed for several N fractions. As N applied increased, mean concentrations of total N (TN), total soluble N (TSN), soluble nonprotein (SNPN), and soluble protein N (SPN) increasedm first‐harvest herbage. Mean concentrations of SPN increased 10.8% at 300 kg N/ha and 13.3% at 700 kg over that in alfalfa not fertilized with N. As the K level increased, mean concentrations of TN, TSN, and SNPN decreased in first‐harvest herbage. Fertilization with K did not affect concentration of SPN. Mean concentrations of TN, TSN, and SNPN in Saranac and TN, TSN, and SPN in 520 first‐harvest herbage were slightly but significantly greater than those in Ramsey. Cultivars did not affect concentrations of N fractions in secondharvest herbage. We found no evidence that fertilization of alfalfa with K or N reduced its apparent bloat potential, as estimated by SPN release. Instead, we showed that alfalfa fertilized with high levels of N had a slightly increased apparent bloat potential.
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Claims 10
Claim · model-written summary
Nitrogen application increased total N, total soluble N, soluble nonprotein N, and soluble protein N in first-harvest alfalfa.
- Taxon
- alfalfa
Medicago sativae1 - Treatment
- N appliede0
- Outcome
- mean concentrations of total N (TN), total soluble N (TSN), soluble nonprotein (SNPN), and soluble protein N (SPN)e0
- Direction
- increasedme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“As N applied increased, mean concentrations of total N (TN), total soluble N (TSN), soluble nonprotein (SNPN), and soluble protein N (SPN) increasedm first‐harvest herbage”
e1“alfalfa ( Medicago sativa L.)”
Claim · model-written summary
Soluble protein N rose 10.8% at 300 kg N/ha relative to unfertilized alfalfa.
- Taxon
- alfalfa
Medicago sativae0 - Treatment
- 300 kg N/hae0
- Outcome
- Mean concentrations of SPNe0
- Direction
- increasede0
- Value
- 10.8%e0
- Comparator
- alfalfa not fertilized with Ne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Mean concentrations of SPN increased 10.8% at 300 kg N/ha and 13.3% at 700 kg over that in alfalfa not fertilized with N”
Claim · model-written summary
Soluble protein N rose 13.3% at 700 kg N/ha relative to unfertilized alfalfa.
- Taxon
- alfalfa
Medicago sativae0 - Treatment
- 700 kge0
- Outcome
- Mean concentrations of SPNe0
- Direction
- increasede0
- Value
- 13.3%e0
- Comparator
- alfalfa not fertilized with Ne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Mean concentrations of SPN increased 10.8% at 300 kg N/ha and 13.3% at 700 kg over that in alfalfa not fertilized with N”
Claim · model-written summary
Increasing potassium reduced total N, total soluble N, and soluble nonprotein N in first-harvest alfalfa.
- Taxon
- alfalfa
Medicago sativae1 - Treatment
- K levele0
- Outcome
- mean concentrations of TN, TSN, and SNPNe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“As the K level increased, mean concentrations of TN, TSN, and SNPN decreased in first‐harvest herbage”
e1“alfalfa ( Medicago sativa L.)”
Claim · model-written summary
Potassium fertilization did not change soluble protein N concentration.
- Taxon
- alfalfa
Medicago sativae1 - Treatment
- Fertilization with Ke0
- Outcome
- concentration of SPNe0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Fertilization with K did not affect concentration of SPN”
e1“alfalfa ( Medicago sativa L.)”
Claim · model-written summary
Saranac exceeded Ramsey for first-harvest total N, total soluble N, and soluble nonprotein N.
- Material
- Saranace0
- Outcome
- Mean concentrations of TN, TSN, and SNPNe0
- Direction
- greatere0
- Comparator
- Ramseye0
Not reportedTaxon
Verbatim
e0“Mean concentrations of TN, TSN, and SNPN in Saranac and TN, TSN, and SPN in 520 first‐harvest herbage were slightly but significantly greater than those in Ramsey”
Claim · model-written summary
520 exceeded Ramsey for first-harvest total N, total soluble N, and soluble protein N.
Not reportedTaxon
Verbatim
e0“Mean concentrations of TN, TSN, and SNPN in Saranac and TN, TSN, and SPN in 520 first‐harvest herbage were slightly but significantly greater than those in Ramsey”
Claim · model-written summary
Cultivars did not differ in second-harvest nitrogen fractions.
- Taxon
- alfalfa
Medicago sativae1 - Outcome
- concentrations of N fractionse0
- Direction
- did not affecte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Cultivars did not affect concentrations of N fractions in secondharvest herbage”
e1“alfalfa ( Medicago sativa L.)”
Claim · model-written summary
Neither potassium nor nitrogen fertilization reduced apparent bloat potential as estimated by soluble protein N release.
- Taxon
- alfalfa
Medicago sativae0 - Treatment
- fertilization of alfalfa with K or Ne0
- Outcome
- apparent bloat potentiale0
- Direction
- no evidencee0
- Qualifier
- as estimated by SPN releasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“We found no evidence that fertilization of alfalfa with K or N reduced its apparent bloat potential, as estimated by SPN release”
Claim · model-written summary
High nitrogen fertilization slightly increased apparent bloat potential.
- Taxon
- alfalfa
Medicago sativae0 - Treatment
- high levels of Ne0
- Outcome
- apparent bloat potentiale0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“alfalfa fertilized with high levels of N had a slightly increased apparent bloat potential”
The same questions in other papers
nitrogen · physiology: osmolytes · Medicago sativa (alfalfa)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
fertilization · physiology: osmolytes · Medicago sativa (alfalfa)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- no effect
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.