Abstract
When leaf blades of fully expanded second leaves of barley (cv. Prior) were excised and incubated with the cut end in a 20 milligram per liter solution of abscisic acid, they accumulated proline at the rate of about 1 micromole per hour per gram fresh weight after a 3- to 4-hour lag. This accumulation occurred reproducibly only if leaves were pretreated by placing the cut end in a solution consisting of 50 millimolar sucrose and 1 millimolar glutamate. Treated leaves were taken from plants which had been in the light for 24 hours.Abscisic acid caused a stimulation of proline synthesis from glutamic acid. Proline oxidation rates were similar in leaves incubated in abscisic acid and in water even though the proline level in abscisic acid-treated leaves was 2.5 times the level in the water-treated controls. The incorporation of proline into protein was not affected by abscisic acid.These results are interpreted to indicate that the metabolic cause of abscisic acid-induced proline accumulation is a stimulation of proline synthesis from glutamic acid. Inhibition of the utilization of proline by oxidation and protein synthesis does not contribute to proline accumulation the way it does in drought-stressed leaves.
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Claims 6
Claim · model-written summary
Excised barley leaves incubated in abscisic acid accumulated proline at about 1 micromole per hour per gram fresh weight after a 3- to 4-hour lag.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Material
- cv. Priore0
- Organ / stage
- fully expanded second leavese0
- Treatment
- 20 milligram per liter solution of abscisic acide0
- Outcome
- prolinee0
- Direction
- accumulatede0
- Value
- about 1 micromole per hour per gram fresh weighte0
- Qualifier
- 3- to 4-hour lage0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“leaf blades of fully expanded second leaves of barley (cv. Prior) were excised and incubated with the cut end in a 20 milligram per liter solution of abscisic acid, they accumulated proline at the rate of about 1 micromole per hour per gram fresh weight after a 3- to 4-hour lag”
Claim · model-written summary
Reproducible proline accumulation required pretreatment with sucrose and glutamate.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- 50 millimolar sucrose and 1 millimolar glutamatee0
- Outcome
- accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This accumulation occurred reproducibly only if leaves were pretreated by placing the cut end in a solution consisting of 50 millimolar sucrose and 1 millimolar glutamate”
t0“Barley Leaves”
Claim · model-written summary
Abscisic acid stimulated proline synthesis from glutamic acid in barley leaves.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- Abscisic acide0
- Outcome
- proline synthesis from glutamic acide0
- Direction
- stimulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Abscisic acid caused a stimulation of proline synthesis from glutamic acid”
t0“Barley Leaves”
Claim · model-written summary
Proline oxidation rates were similar in abscisic acid and water treated barley leaves.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- abscisic acide0
- Outcome
- Proline oxidation ratese0
- Direction
- similare0
- Comparator
- watere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Proline oxidation rates were similar in leaves incubated in abscisic acid and in water”
t0“Barley Leaves”
Claim · model-written summary
Proline level in abscisic acid treated barley leaves was 2.5 times that in water treated controls.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- abscisic acid-treatede0
- Outcome
- proline levele0
- Direction
- higherinferrede0
- Value
- 2.5 timese0
- Comparator
- water-treated controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the proline level in abscisic acid-treated leaves was 2.5 times the level in the water-treated controls”
t0“Barley Leaves”
Claim · model-written summary
Abscisic acid did not affect incorporation of proline into protein in barley leaves.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- abscisic acide0
- Outcome
- incorporation of proline into proteine0
- Direction
- not affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The incorporation of proline into protein was not affected by abscisic acid”
t0“Barley Leaves”