Abstract
The regeneration rate of wheat immature embryo varies among genotypes, howbeit many elite agriculture wheat varieties have low regeneration rates. Optimization of tissue culture conditions and attempts of adding signal molecules are effective ways to increase plant regeneration rate. Inter-culture is one of ways that have not been investigated in plant tissue culture. Moreover, the use of arabinogalactan proteins (AGPs) and hydrogen peroxide (H2O2) have been reported to increase regeneration rate in a few plant species other than wheat. The current research pioneeringly uses inter-culture of immature embryos of different wheat genotypes, and also investigates impacts of AGP and H2O2 on the induction of embryogenic calli and plant regeneration. As a result, high-frequency regeneration wheat cultivars Kenong 199 (KN199) and Xinchun 9 (XC9), together with low-frequency regeneration wheat line Chinese Spring (CS), presented striking increase in the induction of embryogenic calli and plant regeneration rate of CS through inter-culture strategy, up to 52.19 and 67.98%, respectively. Adding 50 to 200 mg L−1 AGP or 0.005 to 0.01 ‰ H2O2 to the callus induction medium, enhanced growth of embryogenic calli and plant regeneration rate in quite a few wheat genotypes. At 50 mg L−1 AGP application level in callus induction medium plant regeneration rates of 8.49, 409.06 and 283.16% were achieved for Jimai 22 (JM22), Jingdong 18 (JD18) and Yangmai 18 (YM18), respectively; whereas at 100 mg L−1 AGP level, CS (105.44%), Chuannong 16 (CN16) (80.60%) and Ningchun 4 (NC4) (62.87%) acted the best. Moreover CS (79.05%), JM22 (7.55%), CN16 (101.87%), YM18 (365.56%), Yangmai 20 (YM20) (10.48%), and CB301 (187.40%) were more responsive to 0.005 ‰ of H2O2, and NC4 (35.37%) obtained the highest shoot regeneration rates at 0.01 ‰ of H2O2. Overall, these two methods, inter-culture and AGP (or H2O2) application, can be further applied to wheat transgenic research.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 18
Claim · model-written summary
Inter-culture increased embryogenic callus induction in Chinese Spring to 52.19%.
- Taxon
- wheat
Triticum aestivume0 - Material
- Chinese Springe0
- Treatment
- inter-culture strategye0
- Outcome
- induction of embryogenic callie0
- Direction
- increasee0
- Value
- 52.19e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“wheat line Chinese Spring (CS), presented striking increase in the induction of embryogenic calli and plant regeneration rate of CS through inter-culture strategy, up to 52.19 and 67.98%, respectively”
Claim · model-written summary
Inter-culture increased Chinese Spring plant regeneration rate to 67.98%.
- Taxon
- wheatTriticum aestivume0
- Material
- Chinese Springe0
- Treatment
- inter-culture strategye0
- Outcome
- plant regeneration ratee0
- Direction
- increasee0
- Value
- 67.98%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“wheat line Chinese Spring (CS), presented striking increase in the induction of embryogenic calli and plant regeneration rate of CS through inter-culture strategy, up to 52.19 and 67.98%, respectively”
Claim · model-written summary
AGP enhanced embryogenic callus growth and regeneration in several wheat genotypes.
- Taxon
- wheatTriticum aestivume0
- Treatment
- 50 to 200 mg L−1 AGPe0
- Outcome
- growth of embryogenic calli and plant regeneration ratee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Adding 50 to 200 mg L−1 AGP or 0.005 to 0.01 ‰ H2O2 to the callus induction medium, enhanced growth of embryogenic calli and plant regeneration rate in quite a few wheat genotypes”
Claim · model-written summary
Hydrogen peroxide enhanced embryogenic callus growth and regeneration in several wheat genotypes.
- Taxon
- wheatTriticum aestivume0
- Treatment
- 0.005 to 0.01 ‰ H2O2e0
- Outcome
- growth of embryogenic calli and plant regeneration ratee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Adding 50 to 200 mg L−1 AGP or 0.005 to 0.01 ‰ H2O2 to the callus induction medium, enhanced growth of embryogenic calli and plant regeneration rate in quite a few wheat genotypes”
Claim · model-written summary
At 50 mg L−1 AGP, Jimai 22 regeneration was 8.49%.
- Taxon
- wheatTriticum aestivumt0
- Material
- Jimai 22e0
- Treatment
- 50 mg L−1 AGPe0
- Outcome
- plant regeneration ratese0
- Value
- 8.49e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“At 50 mg L−1 AGP application level in callus induction medium plant regeneration rates of 8.49, 409.06 and 283.16% were achieved for Jimai 22 (JM22), Jingdong 18 (JD18) and Yangmai 18 (YM18), respectively”
Claim · model-written summary
At 50 mg L−1 AGP, Jingdong 18 regeneration was 409.06%.
- Taxon
- wheatTriticum aestivumt0
- Material
- Jingdong 18e0
- Treatment
- 50 mg L−1 AGPe0
- Outcome
- plant regeneration ratese0
- Value
- 409.06e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“At 50 mg L−1 AGP application level in callus induction medium plant regeneration rates of 8.49, 409.06 and 283.16% were achieved for Jimai 22 (JM22), Jingdong 18 (JD18) and Yangmai 18 (YM18), respectively”
Claim · model-written summary
At 50 mg L−1 AGP, Yangmai 18 regeneration was 283.16%.
- Taxon
- wheatTriticum aestivumt0
- Material
- Yangmai 18e0
- Treatment
- 50 mg L−1 AGPe0
- Outcome
- plant regeneration ratese0
- Value
- 283.16%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“At 50 mg L−1 AGP application level in callus induction medium plant regeneration rates of 8.49, 409.06 and 283.16% were achieved for Jimai 22 (JM22), Jingdong 18 (JD18) and Yangmai 18 (YM18), respectively”
Claim · model-written summary
At 100 mg L−1 AGP, Chinese Spring regeneration was 105.44%.
- Taxon
- wheatTriticum aestivumt0
- Material
- Chinese Springe1
- Treatment
- 100 mg L−1 AGPe0
- Outcome
- acted the beste0
- Value
- 105.44%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“at 100 mg L−1 AGP level, CS (105.44%), Chuannong 16 (CN16) (80.60%) and Ningchun 4 (NC4) (62.87%) acted the best”
e1“Chinese Spring (CS)”
Claim · model-written summary
At 100 mg L−1 AGP, Chuannong 16 regeneration was 80.60%.
- Taxon
- wheatTriticum aestivumt0
- Material
- Chuannong 16e0
- Treatment
- 100 mg L−1 AGPe0
- Outcome
- acted the beste0
- Value
- 80.60%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“at 100 mg L−1 AGP level, CS (105.44%), Chuannong 16 (CN16) (80.60%) and Ningchun 4 (NC4) (62.87%) acted the best”
Claim · model-written summary
At 100 mg L−1 AGP, Ningchun 4 regeneration was 62.87%.
- Taxon
- wheatTriticum aestivumt0
- Material
- Ningchun 4e0
- Treatment
- 100 mg L−1 AGPe0
- Outcome
- acted the beste0
- Value
- 62.87%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“at 100 mg L−1 AGP level, CS (105.44%), Chuannong 16 (CN16) (80.60%) and Ningchun 4 (NC4) (62.87%) acted the best”
Claim · model-written summary
At 0.005 ‰ H2O2, Chinese Spring response was 79.05%.
- Taxon
- wheatTriticum aestivumt0
- Material
- Chinese Springe1
- Treatment
- 0.005 ‰ of H2O2e0
- Outcome
- more responsivee0
- Value
- 79.05%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“Moreover CS (79.05%), JM22 (7.55%), CN16 (101.87%), YM18 (365.56%), Yangmai 20 (YM20) (10.48%), and CB301 (187.40%) were more responsive to 0.005 ‰ of H2O2”
e1“Chinese Spring (CS)”
Claim · model-written summary
At 0.005 ‰ H2O2, Jimai 22 response was 7.55%.
- Taxon
- wheatTriticum aestivumt0
- Material
- Jimai 22e1
- Treatment
- 0.005 ‰ of H2O2e0
- Outcome
- more responsivee0
- Value
- 7.55%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“Moreover CS (79.05%), JM22 (7.55%), CN16 (101.87%), YM18 (365.56%), Yangmai 20 (YM20) (10.48%), and CB301 (187.40%) were more responsive to 0.005 ‰ of H2O2”
e1“Jimai 22 (JM22)”
Claim · model-written summary
At 0.005 ‰ H2O2, Chuannong 16 response was 101.87%.
- Taxon
- wheatTriticum aestivumt0
- Material
- Chuannong 16e1
- Treatment
- 0.005 ‰ of H2O2e0
- Outcome
- more responsivee0
- Value
- 101.87%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“Moreover CS (79.05%), JM22 (7.55%), CN16 (101.87%), YM18 (365.56%), Yangmai 20 (YM20) (10.48%), and CB301 (187.40%) were more responsive to 0.005 ‰ of H2O2”
e1“Chuannong 16 (CN16)”
Claim · model-written summary
At 0.005 ‰ H2O2, Yangmai 18 response was 365.56%.
- Taxon
- wheatTriticum aestivumt0
- Material
- Yangmai 18e1
- Treatment
- 0.005 ‰ of H2O2e0
- Outcome
- more responsivee0
- Value
- 365.56%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“Moreover CS (79.05%), JM22 (7.55%), CN16 (101.87%), YM18 (365.56%), Yangmai 20 (YM20) (10.48%), and CB301 (187.40%) were more responsive to 0.005 ‰ of H2O2”
e1“Yangmai 18 (YM18)”
Claim · model-written summary
At 0.005 ‰ H2O2, Yangmai 20 response was 10.48%.
- Taxon
- wheatTriticum aestivumt0
- Material
- Yangmai 20e0
- Treatment
- 0.005 ‰ of H2O2e0
- Outcome
- more responsivee0
- Value
- 10.48%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“Moreover CS (79.05%), JM22 (7.55%), CN16 (101.87%), YM18 (365.56%), Yangmai 20 (YM20) (10.48%), and CB301 (187.40%) were more responsive to 0.005 ‰ of H2O2”
Claim · model-written summary
At 0.005 ‰ H2O2, CB301 response was 187.40%.
- Taxon
- wheatTriticum aestivumt0
- Material
- CB301e0
- Treatment
- 0.005 ‰ of H2O2e0
- Outcome
- more responsivee0
- Value
- 187.40%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“Moreover CS (79.05%), JM22 (7.55%), CN16 (101.87%), YM18 (365.56%), Yangmai 20 (YM20) (10.48%), and CB301 (187.40%) were more responsive to 0.005 ‰ of H2O2”
Claim · model-written summary
Ningchun 4 had its highest shoot regeneration at 0.01 ‰ H2O2, at 35.37%.
- Taxon
- wheatTriticum aestivumt0
- Material
- Ningchun 4e1
- Treatment
- 0.01 ‰ of H2O2e0
- Outcome
- shoot regeneration ratese0
- Direction
- higheste0
- Value
- 35.37%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“NC4 (35.37%) obtained the highest shoot regeneration rates at 0.01 ‰ of H2O2”
e1“Ningchun 4 (NC4)”
Claim · model-written summary
Authors suggest applying inter-culture and AGP or H2O2 in wheat transgenic research.
- Taxon
- wheatTriticum aestivume0
- Outcome
- can be further applied to wheat transgenic researche0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these two methods, inter-culture and AGP (or H2O2) application, can be further applied to wheat transgenic research”
The same questions in other papers
hydrogen peroxide · growth · Triticum aestivum (wheat)Results differ
15 papers in the same direction as this paper · 5 in the opposite direction
- positive
- negative
- no effect
17 papers more in this group
See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.